Isolation and characterization of Avibacterium paragallinarum with different nicotinamide adenine dinucleotide requirements

Jeong, Ok-Mi; Kang, Min-Su; Jeon, Byung-Woo; Choi, Byung-Kook; Kwon, Yong-Kuk; Yoon, So-Youn; Blackall, P. J.; Lee, Heesoo; Jung, Suk-Chan; Kim, Jae Hong

doi:10.1016/j.vetmic.2017.05.005

Cited by 14 publications

(12 citation statements)

References 20 publications

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“…All six isolates were able to ferment lactose, maltose, mannitol, and sorbitol, the similar results were obtained by other studies (Akter et al 2014;Wahyuni et al 2018;Tangkonda et al 2019). All six isolates were unable to ferment galactose, this result was in accordance with other studies (Patil et al 2016;Jeong et al 2017;Feberwee et al 2019). All the six isolates of A. paragallinarum were further confirmed with speciesspecific HPG-2 PCR test.…”

Section: Discussionsupporting

confidence: 90%

“…Results of biochemical test (Table 1) showed that 6 isolates had negative results in catalase, oxidase, indole, VP, urease tests and were not motile. A similar finding was also reported by (Patil et al 2016;Jeong et al 2017;Wahyuni et al 2018). All six isolates were able to ferment lactose, maltose, mannitol, and sorbitol, the similar results were obtained by other studies (Akter et al 2014;Wahyuni et al 2018;Tangkonda et al 2019).…”

Section: Discussionsupporting

confidence: 90%

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Short Communication: PCR detection of Avibacterium paragallinarum from layers with infectious coryza symptoms in poultry farms of Sleman District, Indonesia

et al. 2021

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Abstract. Fauziah I, Asmara W, Wahyuni AETH, Widayanti R. 2021. Short Communication: PCR detection of Avibacterium paragallinarum from layers with infectious coryza symptoms in poultry farms of Sleman District, Indonesia. Biodiversitas 22: 4890-4894. Infectious coryza (IC), caused by Avibacterium paragallinarum, is a contagious and infectious respiratory tract disease that affects the commercial poultry industry. Molecular techniques, such as species-specific PCR, HPG-2 PCR are mostly used for the detection of A. paragallinarum. The current research was carried out to isolate A. paragallinarum from the layers of infectious coryza signs in Sleman District, special region of Yogyakarta, followed by PCR confirmation of the identified bacteria. Nine field isolates were observed and determined based on their colony and cell morphology. All isolates were characterized biochemically and confirmed with species-specific HPG-2 PCR for A. paragallinarum. Out of 9 isolates, 6 (66.7%) isolates were biochemically identified as A. paragallinarum and confirmed by HPG-2 PCR.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 90%

Short Communication: PCR detection of Avibacterium paragallinarum from layers with infectious coryza symptoms in poultry farms of Sleman District, Indonesia

et al. 2021

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“…Two eligible studies from Thailand (Chukiatsiri et al., 2012 ) and South Korea (Jeong et al., 2017 ) reported susceptibility data for A. paragallinarum , but notably only in 18 and 20 chicken isolates from unknown production type and layers, respectively. The two studies used the same breakpoints (Jeong referred to Chukiatsiri), which included various veterinary CLSI breakpoints and interpretive criteria provided by the disk manufacturer.…”

Section: Assessmentmentioning

confidence: 99%

Assessment of animal diseases caused by bacteria resistant to antimicrobials: Poultry

Nielsen¹,

Bicout²,

Calistri³

et al. 2021

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In this opinion, the antimicrobial‐resistant bacteria responsible for transmissible diseases that constitute a threat to poultry health have been assessed. The assessment has been performed following a methodology based on information collected by an extensive literature review and expert judgement. Details of the methodology used for this assessment are explained in a separate opinion. A global state of play is provided for: Avibacterium (Haemophilus) paragallinarum, Bordetella avium, Clostridium perfringens, Enterococcus faecalis and Enterococcus cecorum, Erysipelothrix rhusiopathiae, Escherichia coli, Gallibacterium spp., Mycoplasma synoviae, Ornithobacterium rhinotracheale, Pasteurella multocida, Riemerella anatipestifer and Staphylococcus aureus. Among those bacteria, EFSA identified Escherichia coli, Enterococcus faecalis and Enterococcus cecorum with ≥ 66% certainty as being the most relevant antimicrobial resistant bacteria in the EU based on the available evidence. The animal health impact of these most relevant bacteria, and their eligibility for being listed and categorised within the Animal Health Law Framework, will be assessed in separate scientific opinions.

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“…The nasal exudate samples were cultured on a chocolate agar plate (Oxoid™, Basingstoke, UK), added with 5% sheep blood at 80°C, and incubated in an anaerobic jar for 24-48 h at 37°C. Identifications were made based on bacterial colony and cell morphology with Gram staining and biochemical tests (catalase test, oxidase test, urease test, motility test on semisolid media, indole test, and carbohydrate fermentation tests using lactose, maltose, mannitol, and sorbitol) [11][12][13].…”

Section: Isolation and Identification Of A Paragallinarummentioning

confidence: 99%

Antimicrobial sensitivity of Avibacterium paragallinarum isolates from layers in the special region of Yogyakarta, Indonesia

2021

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Background and Aim: Infectious coryza (IC) is an upper respiratory disease of chicken caused by Avibacterium paragallinarum. Its clinical symptoms are swollen face and malodorous sinus exudate. This study was conducted to determine the antimicrobial sensitivity of A. paragallinarum isolates from layers in the Special Region of Yogyakarta, Indonesia. Materials and Methods: The samples used in this study were 30 layers that showed IC symptoms. The colony and cell morphology were observed with Gram staining; then, biochemical tests (catalase, oxidase, urease, indole, and motility tests, and carbohydrate fermentation tests using lactose, maltose, mannitol, and sorbitol) were performed to the suspected colony to identify A. paragallinarum. An antibiotic sensitivity test was performed using several antibiotic disks against A. paragallinarum isolates that were cultured on Mueller-Hinton Agar. Results: Out of 30 samples, 24 samples (80%) were found positive for A. paragallinarum. All isolates were sensitive to ampicillin (AMP) and amoxicillin (AML) (100%), and chloramphenicol (C) (91.6%). The antibiotics with intermediate sensitivity were enrofloxacin (79.2%), fosfomycin (75%), and ciprofloxacin (54.2%). The isolates were most resistant to erythromycin (100%), followed by tetracycline (87.5%), streptomycin (83.3%), doxycycline and kanamycin (70.8%), and trimethoprim (62.5%). Conclusion: Out of the total samples, 24 samples (80%) from layers with IC symptoms were identified biochemically as A. paragallinarum. It was sensitive to AMP, AML, and C.

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Isolation and characterization of Avibacterium paragallinarum with different nicotinamide adenine dinucleotide requirements

Cited by 14 publications

References 20 publications

Short Communication: PCR detection of Avibacterium paragallinarum from layers with infectious coryza symptoms in poultry farms of Sleman District, Indonesia

Short Communication: PCR detection of Avibacterium paragallinarum from layers with infectious coryza symptoms in poultry farms of Sleman District, Indonesia

Assessment of animal diseases caused by bacteria resistant to antimicrobials: Poultry

Antimicrobial sensitivity of Avibacterium paragallinarum isolates from layers in the special region of Yogyakarta, Indonesia

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