Isolation and Characterization of Diuron-degrading Bacteria from Lotic Surface Water

Batisson, Isabelle; Pesce, Stéphane; Besse-Hoggan, Pascale; Sancelme, Martine; Bohatier, Jacques

doi:10.1007/s00248-007-9241-2

Cited by 35 publications

(26 citation statements)

References 47 publications

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“…PCR products were quantified with the DNA quantification fluorescence assay kit (Sigma-Aldrich, St Louis, MO, USA). Each sample weighing 300 ng were electrophoresed through an 8% polyacrylamide gel (TAE 1.25 Â , urea 7 M, Temed 0.06%, ammonium persulfate 0.0625%) as previously described (Batisson et al, 2007). Band patterns were analyzed using the GelCompare 4.6 software package (Applied Maths, Kortrijk, Belgium).…”

Section: Random Amplification Of Polymorphic Dna-pcr (Rapd-pcr) Fingementioning

confidence: 99%

Unexpected and novel putative viruses in the sediments of a deep-dark permanently anoxic freshwater habitat

et al. 2012

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Morphological diversity, abundance and community structure of viruses were examined in the deep and anoxic sediments of the volcanic Lake Pavin (France). The sediment core, encompassing 130 years of sedimentation, was subsampled every centimeter. High viral abundances were recorded and correlated to prokaryotic densities. Abundances of viruses and prokaryotes decreased with the depth, contrasting the pattern of virus-to-prokaryote ratio. According to fingerprint analyses, the community structure of viruses, bacteria and archaea gradually changed, and communities of the surface (0-10 cm) could be discriminated from those of the intermediate (11-27 cm) and deep (28-40 cm) sediment layers. Viral morphotypes similar to virions of ubiquitous dsDNA viruses of bacteria were observed. Exceptional morphotypes, previously never reported in freshwater systems, were also detected. Some of these resembled dsDNA viruses of hyperthermophilic and hyperhalophilic archaea. Moreover, unusual types of spherical and cubic virus-like particles (VLPs) were observed. Infected prokaryotic cells were detected in the whole sediment core, and their vertical distribution correlated with both viral and prokaryotic abundances. Pleomorphic ellipsoid VLPs were visible in filamentous cells tentatively identified as representatives of the archaeal genus Methanosaeta, a major group of methane producers on earth.

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Section: Random Amplification Of Polymorphic Dna-pcr (Rapd-pcr) Fingementioning

confidence: 99%

Unexpected and novel putative viruses in the sediments of a deep-dark permanently anoxic freshwater habitat

et al. 2012

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“…Several Diuron-degrading bacteria have been isolated from different agricultural soils [12]- [14] and river waters [15].…”

Section: Introductionmentioning

confidence: 99%

Bioremediation of Diuron in Soil Environment: Influence of Cyanobacterial Mat

Safi¹,

Awad²,

El‐Nahhal³

2014

AJPS

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Diuron is a herbicide widely used in the Middle East; its field application creates many environmental problems. This study investigated the bioremediation of Diuron in soil environment by Cyanobacterial mats collected from Gaza Wadi. Various concentrations of Diuron were injected in water saturated soil samples pre-treated with Cyanobacterial mat for several periods. Percent growth of Jews mallow as a test plant was taken as indicator of biodegradation of Diuron. Results showed that Diuron was degraded in soil and degradation was more pronounced when Diuron was incubated with Cyanobacterial in the irrigation water. Larger applied rate of Cyanobacterial mat did not affect the biodegradation of Diuron. These encouraging results suggest that application of Cyanobacterial mat could be a suitable method to remediate soil pollution.

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“…Several diuron-degrading bacteria have been isolated from different agricultural soils (10,13,16,42,53) and river waters (1). Furthermore, it has been shown that different fungi, either obtained from various culture collections or freshly isolated from agricultural soils, are capable of degrading diuron (8,20,29,52).…”

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confidence: 99%

Rapid Mineralization of the Phenylurea Herbicide Diuron by Variovorax sp. Strain SRS16 in Pure Culture and within a Two-Member Consortium

Sørensen

Albers

Aamand

2008

Appl Environ Microbiol

136

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The phenylurea herbicide diuron [N-(3,4-dichlorophenyl)-N,N-dimethylurea] is widely used in a broad range of herbicide formulations, and consequently, it is frequently detected as a major water contaminant in areas where there is extensive use. We constructed a linuron [N-(3,4-dichlorophenyl)-N-methoxy-N-methylurea]-and diuron-mineralizing two-member consortium by combining the cooperative degradation capacities of the diuron-degrading organism Arthrobacter globiformis strain D47 and the linuron-mineralizing organism Variovorax sp. strain SRS16. Neither of the strains mineralized diuron alone in a mineral medium, but combined, the two strains mineralized 31 to 62% of the added [ring-U-14 C]diuron to 14 CO 2 , depending on the initial diuron concentration and the cultivation conditions. The constructed consortium was used to initiate the degradation and mineralization of diuron in soil without natural attenuation potential. This approach led to the unexpected finding that Variovorax sp. strain SRS16 was able to mineralize diuron in a pure culture when it was supplemented with appropriate growth substrates, making this strain the first known bacterium capable of mineralizing diuron and representatives of both the N,N-dimethyl-and N-methoxy-N-methyl-substituted phenylurea herbicides. The ability of the coculture to mineralize microgram-per-liter levels of diuron was compared to the ability of strain SRS16 alone, which revealed the greater extent of mineralization by the two-member consortium (31 to 33% of the added [ring-U-14 C]diuron was mineralized to 14 CO 2 when 15.5 to 38.9 g liter ؊1 diuron was used). These results suggest that the consortium consisting of strains SRS16 and D47 could be a promising candidate for remediation of soil and water contaminated with diuron and linuron and their shared metabolite 3,4-dichloroaniline.

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Isolation and Characterization of Diuron-degrading Bacteria from Lotic Surface Water

Cited by 35 publications

References 47 publications

Unexpected and novel putative viruses in the sediments of a deep-dark permanently anoxic freshwater habitat

Unexpected and novel putative viruses in the sediments of a deep-dark permanently anoxic freshwater habitat

Bioremediation of Diuron in Soil Environment: Influence of Cyanobacterial Mat

Rapid Mineralization of the Phenylurea Herbicide Diuron by Variovorax sp. Strain SRS16 in Pure Culture and within a Two-Member Consortium

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