Isolation and Culture of Hexamita Free of Bacteria

The axenic cultivation of Hexamita inflata from Crassostrea virginica

“…The complex nutrition and anaerobiosis of species of Hexamita has generally made laboratory cultivation difficult. Thus a number of workers (Bishop 1933;Hughes and Zander 1954;and Scheltema 1962) reported attempts to cultivate species of Hexamita but did not obtain axenic cultures on artificial media. Althougll Khouw (1962) briefly described the axenic cultivation of H. inflata, the first complete report of an axenic culture of a species of Hexarnita was that of Uzmann and Hayduk (1963).…”