Isolation and identification of an antioxidant collagen peptide from skipjack tuna (<i>Katsuwonus pelamis</i>) bone

Ding, Ding; Du, Bowei; Zhang, Chao; Zaman, Fakhar; Huang, Yaqin

doi:10.1039/c9ra04665h

Cited by 37 publications

(27 citation statements)

References 46 publications

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“…Several studies of bioactive peptides from collagen by-products in the fisheries industry are as follows. Antioxidant activities have been studied for the hydrolysates/peptides: salmon scales [22], skipjack tuna bone [23], seabass skin [24], Spanish mackerel skin [25,26], milkfish scales [27], tilapia scales [28], and tilapia skin [29]. In these studies, antioxidant activities were studied using a scavenging activity test against superoxide anion, DPPH (2,2-diphenyl-1-picrylhydrazyl) radical, ABTS (2,2 -azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)) radical, and OH radical, FRAP (ferric reducing antioxidant power) or reducing power assays, and ferrous or metal chelating activity.…”

Section: Introductionmentioning

confidence: 99%

The Bioactivity Prediction of Peptides from Tuna Skin Collagen Using Integrated Method Combining In Vitro and In Silico

Devita

Lioe

Nurilmala

et al. 2021

Foods

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The hydrolysates and peptide fractions of bigeye tuna (Thunnus obesus) skin collagen have been successfully studied. The hydrolysates (HPA, HPN, HPS, HBA, HBN, HBS) were the result of the hydrolysis of collagen using alcalase, neutrase, and savinase. The peptide fractions (PPA, PPN, PPS, PBA, PBN, PBS) were the fractions obtained following ultrafiltration of the hydrolysates. The antioxidant activities of the hydrolysates and peptide fractions were studied using the DPPH method. The effects of collagen types, enzymes, and molecular sizes on the antioxidant activities were analyzed using profile plots analysis. The amino acid sequences of the peptides in the fraction with the highest antioxidant activity were analyzed using LC-MS/MS. Finally, their bioactivity and characteristics were studied using in silico analysis. The hydrolysates and peptide fractions provided antioxidant activity (6.17–135.40 µmol AAE/g protein). The lower molecular weight fraction had higher antioxidant activity. Collagen from pepsin treatment produced higher activity than that of bromelain treatment. The fraction from collagen hydrolysates by savinase treatment had the highest activity compared to neutrase and alcalase treatments. The peptides in the PBN and PPS fractions of <3 kDa had antidiabetic, antihypertensive and antioxidant activities. In conclusion, they have the potential to be used in food and health applications.

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Section: Introductionmentioning

confidence: 99%

The Bioactivity Prediction of Peptides from Tuna Skin Collagen Using Integrated Method Combining In Vitro and In Silico

Devita

Lioe

Nurilmala

et al. 2021

Foods

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“…Pepsin shows preferential cleavage of aromatic residues in either position of the peptide bond [47]. Trypsin hydrolyzes the peptide and ester bonds formed by the carboxyl group of the base amino acid, including Arg and Lys [47,48]. Overall, all of these proteases affected collagen yields.…”

Section: Extraction and Yield Of Collagenmentioning

confidence: 99%

“…This collagen also contains other amino acid residues, such as arginine, aspartic acid, cysteine, histidine, hydroxylysine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tyrosine, valine, and imino acids [36]. Several amino acids have been reported to be influential in the activity of free radical scavenging, including Trp, Tyr, Phe, Lys, and Arg [48]. Thus, the DPPH radical scavenging activity shown by our collagen samples was possibly due to the presence of the amino acids arginine, lysine, phenylalanine, and tyrosine.…”

Section: Compounds Absorbances (700 Nm)mentioning

confidence: 99%

Chemical and Antioxidant Characteristics of Skin-Derived Collagen Obtained by Acid-Enzymatic Hydrolysis of Bigeye Tuna (Thunnus obesus)

et al. 2021

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The utilization of bigeye tuna skin as a source of collagen has been increasing the value of these skins. In this study, the quality of the skin was studied first. The skin after 14 h freeze-drying showed a high protein level (65.42% ± 0.06%, db), no histamine and a lack of heavy metals. The collagens were extracted through acid and acid-enzymatic methods. The enzymes used were bromelain, papain, pepsin, and trypsin. The two highest-yield collagens were pepsin-soluble collagen (PSC) and bromelain-soluble collagen (BSC). Both were type I collagen, based on SDS-PAGE and FTIR analysis. They dissolved very well in dimethyl sulfoxide and distilled water. The pH ranges were 4.60–4.70 and 4.30–4.40 for PSC and BSC, respectively. PSC and BSC were free from As, Cd, Co, Cr, Cu, and Pb. They showed antioxidant activities, as determined by the DPPH method and the reducing power method. In conclusion, bigeye tuna skin shows good potential as an alternative source of mammalian collagen. Although further work is still required, PSC and BSC showed the potential to be further used as antioxidant compounds in food applications. Other biological tests of these collagens might also lead to other health applications.

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“…Antioxidant peptides have been demonstrated to inhibit excessive ROS (Zhang et al., 2019). There are also many antioxidant peptides found in food, such as SSGPPVPGPMGPMGPR from skipjack tuna bone (Ding, Du, Zhang, Zaman, & Huang, 2019), QGPIVLNPWDQVKR from fermented milk (Tonolo et al., 2020), and VYLPR from egg white protein (Zhang et al., 2019). However, most of them were analyzed single biological activity.…”

Section: Introductionmentioning

confidence: 99%

Bifunctional peptides with antioxidant and angiotensin‐converting enzyme inhibitory activity in vitro from egg white hydrolysates

Liu

et al. 2020

J Food Biochem

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Oxidative stress is frequently associated with hypertension. However, the studies on antihypertensive peptides usually focus on ACE inhibitory activity and ignore antioxidant activity of bioactive peptides. In our research, we optimized enzymatic hydrolysis condition of egg white protein. After that we evaluated both the antioxidant and the ACE‐inhibitory activity in vitro of egg white hydrolysates and its purified fractions. Four peptides, LAPYK, SVIRW, PKSVIRW, and ADWAK, which can match to ovotransferrin were identified from fraction with the highest bioactivity. Among them, LAPYK possessed the strongest activity with ABTS scavenging IC50 of 5.29 ± 0.24 µM, ORAC of 1.50 ± 0.04 µM TE/µM, ACE‐inhibitory IC50 of 12.65 ± 1.34 µM. For further, we explored inhibitory mechanism of LAPYK against ACE. Research on bifunctional peptides is helpful for the application of antihypertensive peptides. Practical applications At present, the therapeutic drugs for hypertension usually focus on a single target, but the pathogenesis of hypertension is multifactorial, and the combined treatment of multiple targets may have a better therapeutic effect. Compared with commercially available drugs, food‐derived bioactive peptides have lower side effects, as well as exhibit multi‐bioactivities more easily. Exploration of bifunctional peptides with both ACE inhibitory and antioxidant activity provides new ideas for the treatment of hypertension.

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Isolation and identification of an antioxidant collagen peptide from skipjack tuna (Katsuwonus pelamis) bone

Abstract: A novel antioxidant peptide was isolated from skipjack tuna bone collagen.

Cited by 37 publications

References 46 publications

The Bioactivity Prediction of Peptides from Tuna Skin Collagen Using Integrated Method Combining In Vitro and In Silico

The Bioactivity Prediction of Peptides from Tuna Skin Collagen Using Integrated Method Combining In Vitro and In Silico

Chemical and Antioxidant Characteristics of Skin-Derived Collagen Obtained by Acid-Enzymatic Hydrolysis of Bigeye Tuna (Thunnus obesus)

Bifunctional peptides with antioxidant and angiotensin‐converting enzyme inhibitory activity in vitro from egg white hydrolysates

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