Isolation and Identification of Avibacterium paragallinarum from Layer Chickens in Gazipur, Bangladesh

Abstract

An investigation was conducted for isolation, identification and determination of antibiotic sensitivity of Avibacterium paragallinarun, the causal agent of infectious coryza, from layer chickens. A total of 21 samples with characteristic symptoms of the disease were collected from a Hatchery of Gazipur. Tissue specimens obtained aseptically from swollen infra orbital sinus and tracheal swab were processed, of which, 3 were found positive while the rest 18 were negative. Isolation of bacteria was performed by first putting the specimen in Nicotinamide adenine dinucleotide (NAD) enriched phosphate buffer broth, anaerobically incubated for 24 hours followed by culturing loopful of broth on Blood agar (BA) and Chocolate agar (CA) plates enriched with NAD and streaked with feeder organism of Staphylococcus. aureus. On 24 hours of anaerobic incubation (candle jar method), dew drop satellite colonies of A. paragallinarum were visible on the culture plates. Cultural characteristics of bacteria as well as their staining, morphological, motility and biochemical properties such as sugar fermentation, MR and V-P tests, Indole production and catalase tests were recorded for identification. Further, antibiogram study revealed that the isolates were sensitive to Ciprofloxacin, Chloramphenicol and Gentamicin but resistant to Ampicillin, Amoxycillin, Oxytetracycline, Erythromycin and Sulphamethoxazole. DOI: http://dx.doi.org/10.3329/mh.v3i1.19769 Microbes and Health, June 2014. 3(1): 9-11

Epidemic behavior of the etiological agent of infectious coryza in layer chicken of Bangladesh with isolation, identification and pathogenicity study

“…One of the reasons for the success of survival for this bacterium is that after recovering from infection, birds become carriers of the bacterium, therefore aiding the spread of A. paragallinarum (De Blieck, 1948). In Bangladesh, the information on IC is very scanty except Talha et al 2001, Akter, 2012and Ali et al 2013.These investigators focused on preliminary isolation, identification and pathogenicity study for IC. For the effective control of a specific disease of a specific host in a country must rely on the geographical and epidemiological information based on age, sex, breed, spatial and temporal differences.…”

Pathogenesis of Infectious Coryza in Chickens (Gallus gallus) by Avibacterium paragallinarumn Isolate of Bangladesh

“…Samples showed Gram-negative, red color, rod shaped bacilli arranged as single or paired (Akter, 2012) (Fig. 11).…”

“…Clinical signs were observed for last 7 days and samples were collected at different times on day (Akter, 2012;Blackall 1989;Page, 1962).…”

“…Re-isolation of A. paragallinarum was performed from nasal passage at day 7 of PI where prominent rhinitis was found following the procedures followed by Akter (2012).…”

“…Pathological study on the disease, isolation and identification of the causal agent; and study of pathogenesis by local isolate of this bacterium in experimental bird will enrich the knowledge to identify the disease rapidly and that will reflect the prevention and control measures of the disease. A local isolate of A. paragallinarum was isolated and identified (Akter, 2012), and the present study was undertaken using this isolate to study the pathogenesis of the disease in experimental chicks.…”