Isolation and screening of lactic acid bacteria from grasshopper gut as novel probiotic candidates to digest cellulose polymer

Abstract

The nutritional needs are the primer need in the earth. Various solution of food security problem such as GMO product and chemical fertilizer, are still not effective to clear it because the global warming annualy reported may cause to the crop failures. In addition, the amount of carbohydrate that can be adsorbed by human body is less. The Cellulose is one of the carbohydrate that human can not adsorb to get glucose. Grasshopper can digest the cellulose of grass because there are bacteria live in their organs to produce the enzyme. The bacteria that have an ability giving advantage in the body are known as probiotic. However, the development of this function has became a great attention. The aim is obtaining grasshopper gut bacteria caracterized as lactic acid bacteria potential as novel probiotic to produce cellulase for human digestion. Methods used are; Isolation of grasshopper gut bacteria, Purification and screening modificated of novel probotic candidates. The bacteria were screened by using modificated medium to detect the enzyme activity and pathogenic possibility as well as SP-SDS method to enumerate the tolerance of the bacteria after treatment in the period. The result shows that two bacteria strains of grasshopper gut bacteria are capable to break down the cellulose in the screening process. The bacteria was also caracterized as the lactid acid bacteria. The activity of gamma haemolytic of the bacteria shows the non-pathogenic property on blood. Bile salt and acid pH condition for 48 hour period was tested on the bacteria shows the high tolerance of life in the digestion. In conclusion, there are strain of grasshopper gut bacteria can be used as novel probiotic candidate to digest cellulose as solution of food security.

Novel Probiotic Isolation of Coconut Water's Helpful Lactic Acid Bacteria Cure Covid-19 Patients

“…But the colony grew one-on-one, not within the "Hallo" area. As done by (Abdullah et al, 2019;Bartkiene et al, 2020;Syukur et al, 2018) Figure 2 shows colonies of lactic acid bacteria grown on the selective medium MRSA + 0.3% CaCO3. Colonies of lactic acid bacteria grow in the middle of the "Halo" area.…”

“…So that the area becomes clear, because this medium is a selective medium for these lactic acid bacteria, by using this medium, it can be ascertained that the one that grows in the Halo area is lactic acid bacteria, so it can be directly identified. As well as done by (Abdullah et al, 2019;Franco et al, 2020). When it wants to be propagated and wants to be scratched on the Petri or to tilt, then the colony is taken in the middle.like figure 3.…”

“…Covid-19 is an acute infection of the respiratory tissue caused by the virus (Abdullah et al, 2019) (Wölfel et al, 2020), especially for comorbid sufferers (Berretta et al, 2020;Middleton et al, 2020;Shen et al, 2020). such as diabetes, heart disease, and hypertension.…”

Grasshopper (Oxya chinensis) Gut Bacteria and their Cellulolytic Activity

“…Previously, Shil et al 23 recovered and identified 15 bacterial isolates from the gut of O. velox. Abdullah et al 41 successfully isolated 80 bacterial types from Valanga nigricornis. Wang et al 42 isolated bacteria from three different grasshoppers: Aiolopus tamulus (31 isolates), Oedaleus decorus asiaticus (32 isolates), and Shirakiacris shirakii (30 isolates).…”

“…Hence, the bacteria found in Jimenez and Hernandez 49 reported that fungi showed greater capability for degrading lignocellulosic materials than bacteria in the gut of wood-feeding Coleoptera, with the genera Trichoderma, Bionectria, and Trametes showing positive results in all the assays performed. Abdullah et al 41 reported a cellulolytic index of 0.9-1.7 for bacterial isolates from the grasshopper V. nigricornis. Ferbiyanto et al 19 isolated bacteria from the gut of the termite Macrotermes gilvus and reported a cellulolytic index of 0.75-2.5.…”