Isolation, identification and antimicrobial susceptibility of<i>Bacteroides fragilis</i>group strains recovered from broiler faeces

Garcia, G D; Carvalho, Maria Auxiliadora Roque de; Diniz, Cláudio Galuppo; Marques, José Luiz Silva Lage; Nicoli, Jacques R.; Farias, Luiz M.

doi:10.1080/00071668.2012.662272

Cited by 16 publications

(8 citation statements)

References 21 publications

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“…30 These loci are found mainly in Roseburia and Bacteroide, 31 especially the latter, which is the predominant bacterial genus in the gut of broilers. 32 SCFA concentrations during fermentation were consistent with growth results but not with enzyme activity. XOS fermentation only generated only USCFA, 33 and branched SCFA (isobutyrate and isovalerate) was an indicator of protein fermentation, 34 which is harmful to intestinal health.…”

Section: ■ Discussionsupporting

confidence: 88%

Combination of Xylanase and Debranching Enzymes Specific to Wheat Arabinoxylan Improve the Growth Performance and Gut Health of Broilers

Lei

Shao

Yin

et al. 2016

J. Agric. Food Chem.

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Arabinoxylan (AX) is the major antinutritional factor of wheat. This study evaluated the synergistic effects of xylanase and debranching enzymes (arabinofuranosidase [ABF] and feruloyl esterase [FAE]) on AX. During in vitro tests, the addition of ABF or FAE accelerated the hydrolysis of water-soluble AX (WE-AX) and water-insoluble AX (WU-AX) and produced more xylan oligosaccharides (XOS) than xylanase alone. XOS obtained from WE-AX stimulated greater proliferation of Lactobacillus brevis and Bacillus subtilis than did fructo-oligosaccharides (FOS) and glucose. During in vivo trials, xylanase increased the average daily growth (ADG), decreased the feed-conversion ratio (FCR), and reduced the digesta viscosity of jejunum and intestinal lesions of broilers fed a wheat-based diet on day 36. ABF or FAE additions further improved these effects. Broilers fed a combination of xylanase, ABF, and FAE exhibited the best growth. In conclusion, the synergistic effects among xylanase, ABF, and FAE increased AX degradation, which improve the growth performance and gut health of broilers.

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Section: ■ Discussionsupporting

confidence: 88%

Combination of Xylanase and Debranching Enzymes Specific to Wheat Arabinoxylan Improve the Growth Performance and Gut Health of Broilers

Lei

Shao

Yin

et al. 2016

J. Agric. Food Chem.

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“…Resistance mechanism against this antibiotic is very similar to facultative aerobics and is achieved by mutating in gyrA related gene or by increased expression of efflux pump. The results of this research show 100% resistance against ciprofloxacin which is in complete accordance with reported results of Garcia et al ( 21 ).…”

Section: Discussionsupporting

confidence: 82%

Determination of Antibiotic Sensitivity of Bacteroid fragilis Isolated from Patients and Healthy Individuals in Imam Reza Center of Medical Teaching and Treatment-Tabriz

Akhi

Shirinzadeh

Ghotaslou

et al. 2013

Jundishapur J Microbiol

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Background:Bacteroides fragilis are among the most important anaerobic bacteria behind most of the anaerobic infections. They have acquired resistance to essential treatment antibiotics of anaerobic infections, more than other anaerobic bacteria. Objectives: The goal of this study is to determine the resistance of isolated B. fragilis against common anaerobic infections treatment antibiotics. Patients and Methods: A total of 188 fecal samples including 59 samples from hospitalized patients, 84 samples from outpatients, and 45 samples from healthy individuals were collected. The samples were cultured in Bacteroides-Bile-Esculine agar and KanamycinVancomycin-Laked Blood media and were incubated in anaerobic atmosphere at 37°C for at least 48 hours. Suspected one millimeter sized colonies with black surroundings, were selected and further studied using MID8, as well as biochemical tests. For MIC determination of antibiotics against isolated B. fragilis, Etest was used. Results: There wasn't any difference between antibiotic resistance patterns of isolated B. fragilis from hospitalized patients or outpatients, including diarrheal and non-diarrheal cases, and resistant pattern of isolates from healthy individuals. All or most of isolated B. fragilis were susceptible to imipenem (100%), metronidazole (95%), rifampin (100%) and piperacillin/tazobactam (95%). On the contrary, they exhibited resistance to other antibiotics such as clindamycin (90%), and chloramphenicol (55%). Conclusions:In the present study, we have figured out that a number of the important anaerobic infections treatment antibiotics have lost partly or totally their effectiveness on B. fragilis.

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“…Another recent report found low densities of Bacteroidales in soils (2.5 log gene copies g Ϫ1 ) and chlorinated tap water (1.4 log gene copies liter Ϫ1 ) (33). Relatively few reports have presented evidence of Bacteroidales densities in chicken and turkey feces (6,34) and in poultry litter (33,35). Additionally, we were unable to find peer-reviewed reports regarding the fate of Bacteroidales originating from poultry feces and deposited in poultry litter.…”

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confidence: 39%

Evidence for Extraintestinal Growth of Bacteroidales Originating from Poultry Litter

Weidhaas

Mantha

Hair

et al. 2015

Appl Environ Microbiol

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bWater quality monitoring techniques that target microorganisms in the order Bacteroidales are potential alternatives to conventional methods for detection of fecal indicator bacteria. Bacteroidales and members of the genus Bacteroides have been the focus of microbial source tracking (MST) investigations for discriminating sources of fecal pollution (e.g., human or cattle feces) in environmental waters. For accurate source apportionment to occur, one needs to understand both the abundance of Bacteroides in host feces and the survival of these host-associated microbial markers after deposition in the environment. Studies were undertaken to evaluate the abundance, persistence, and potential for growth of Bacteroidales originating from poultry litter under oxic and anoxic environmental conditions. Bacteroidales abundance, as determined by quantitative PCR (qPCR) with GenBac primers and probe, increased 2 to 5 log gene copies ml ؊1 and 2 log gene copies g litter ؊1 under most conditions during incubation of poultry litter in a variety of laboratory microcosm and field mesocosm studies. DNA sequencing of the Bacteroidales organisms in the litter identified taxa with sequences corresponding exactly to the GenBac primer and probe sequences and that were closely related to Bacteroides uniformis, B. ovatus, and B. vulgatus. These results suggest that MST studies using qPCR methods targeting Bacteroidales in watersheds that are affected by poultry litter should be interpreted cautiously. Growth of Bacteroidales originating from poultry litter in environmental waters may occur while Bacteroidales growth from other fecal sources declines, thus confounding the interpretation of MST results.

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Isolation, identification and antimicrobial susceptibility ofBacteroides fragilisgroup strains recovered from broiler faeces

Cited by 16 publications

References 21 publications

Combination of Xylanase and Debranching Enzymes Specific to Wheat Arabinoxylan Improve the Growth Performance and Gut Health of Broilers

Combination of Xylanase and Debranching Enzymes Specific to Wheat Arabinoxylan Improve the Growth Performance and Gut Health of Broilers

Determination of Antibiotic Sensitivity of Bacteroid fragilis Isolated from Patients and Healthy Individuals in Imam Reza Center of Medical Teaching and Treatment-Tabriz

Evidence for Extraintestinal Growth of Bacteroidales Originating from Poultry Litter

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