Isolation of active compounds from the leaf extract of patah kemudi (Abroma augusta L.) and its anti-inflammatory activity

Abstract

Patah kemudi (Abroma augusta L.) is one of the shrubs that live on the edge of a river. A. augusta contains several secondary metabolite compounds including alkaloids, triterpenoids, steroids and flavonoids. The aim of this study was to isolate the compounds contained in the leaves of Abroma augusta and determine anti-inflammatory activity. Extraction and fractionation were performed by multilevel maceration. Isolation of compounds was carried out by column chromatography. The anti-inflammatory activity test was done using the Winter method. The n-hexane extract was separated by vacuum column chromatography and continued with gravity column chromatography to obtain isolate 8.1, which was then characterized using UV-Vis and IR spectrophotometer. The results of UV-Vis spectrophotometer on isolate 8.1 showed absorption at a maximum wavelength of 268 indicating a conjugate double bond which may be an aromatic originating from steroid compounds. While, the results of IR spectrophotometer characterization from isolate 8.1 manifested the presence of steroid compounds. The results of the activity test of isolate 8.1 with a dose of 10 mg presented the best anti-inflammatory activity compared to the positive control of diclofenac sodium. From the results of the GCMS shows that isolates contain γ-sitosterol compounds with a value of m/z 414.

Isolation of alkaloids compound of ethanol extract of mangrove perepat (S. alba) root and its antibacterial activity

“…Penelitian mengenai eksplorasi senyawa antibakteri baru yang berasal dari bahan alami terus mengalami perkembangan. Salah satu tumbuhan yang menjadi alternatif sumber senyawa antibakteri tersebut adalah tumbuhan mangrove, Sonneratia alba (Latief et al, 2019;Latief et al, 2020).…”

“…Penelitian tentang kandungan metabolit sekunder yang telah dilakukan sebelumnya pada ekstrak metanol daun S. alba menunjukkan bahwa terdapat berbagai senyawa metabolit sekunder, salah satunya adalah alkaloid (Latief et al, 2019;Latief et al, 2020). Secara farmakologis, alkaloid diketahui memiliki banyak aktivitas biologis seperti antibakteri, antiradang, serta antioksidan.…”

“…Uji aktivitas antimikroba pada ekstrak n-heksan, etil asetat, dan metanol daun dan buah S. alba menunjukkan hasil positif untuk S. aureus, bakteri E. coli, dan jamur C. albicans. Selain itu, ekstrak etil asetat dan metanol dari daun S. alba juga terbukti memiliki aktivitas antimikroba pada bakteri gram positif S. aureus dan B. cereus dan E. coli gram negatif serta pada jamur C. neoformans (Latief et al, 2019;Latief et al, 2020;Latief et al, 2018;Muhaimin et al, 2019;Saad et al, 2012).…”

Anti-inflammatory activities of squalene compound of methanol extract of Abroma augusta L

“…Calculated percent edema and percent inhibition of edema by following the previous study. 4 % udem =…”

“…When had an injury occurs, the body will try to neutralize and eliminate harmful agents from the body and make preparations for tissue repair. 4 The protective response due to damage to the tissue that serves to destroy, reduce, or localize both the injured agent and the injured tissue. The main signs of acute inflammation include swelling or edema, redness, heat, pain, and changes in function.…”