Isolation of Bartonella henselae and Two New Bartonella Subspecies, Bartonella koehlerae Subspecies boulouisii subsp. nov. and Bartonella koehlerae Subspecies bothieri subsp. nov. from Free-Ranging Californian Mountain Lions and Bobcats

Chomel, Bruno B; Molia, Sophie; Kasten, Rickie W.; Borgo, Gina M.; Stuckey, Matthew J.; Maruyama, Soichi; Chang, Chi Chen; Haddad, Nadia; Koehler, Jane E.

doi:10.1371/journal.pone.0148299

Cited by 17 publications

(16 citation statements)

References 44 publications

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“…The genus Bartonella is a relatively diverse group of Gram-negative, facultative intracellular, haemotropic, vector-borne, bacteria that infect a wide-range of mammals and have a global distribution. After infection, the bacteria eventually enter the erythrocytes and endothelial cells and can persist asymptomatically in a wide range of mammalian reservoir hosts such as rodents, insectivores, carnivores, and ungulates [ 13 – 15 ]. The infection is transmitted mainly by arthropod vectors including fleas [ 16 ], sand flies [ 17 ], lice [ 18 ], mites [ 19 ] and ticks [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Bartonella DNA in heart tissues of bats in central and eastern Europe and a review of phylogenetic relations of bat-associated bartonellae

et al. 2018

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BackgroundBats are among the most widely distributed mammals worldwide and can represent hosts or reservoirs for a number of different pathogens. Bartonella spp. are opportunistic bacterial pathogens, which are transmitted by a large variety of arthropods. The aim of this study was to investigate the presence and host-associations of these Gram-negative bacteria in heart tissues of bats collected in four different countries from eastern and central Europe and to analyze their phylogenetic relationship with other bat-associated bartonellae.ResultsThe results of this study show for the first time the presence of Bartonella spp. DNA in heart tissues of bats from central and eastern Europe. The overall prevalence of the infection was 1.38%. Phylogenetic analysis identified four new Bartonella spp. sequences, which were closely related with other Bartonella previously isolated from bats in Europe and North America.ConclusionsThe gltA sequences of Bartonella spp. showed considerable heterogeneity in the phylogenetic analysis resulting in six different clades. Our study demonstrated the presence of Bartonella spp. only in heart tissues of bats from Romania, with two new bat species recorded as hosts (Myotis cf. alcathoe and Pipistrellus pipistrellus).Electronic supplementary materialThe online version of this article (10.1186/s13071-018-3070-7) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Bartonella DNA in heart tissues of bats in central and eastern Europe and a review of phylogenetic relations of bat-associated bartonellae

et al. 2018

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“…Overall, prevalence of Bartonella infections in carnivores was higher compared to Brucella infections. In the studies of over two Feliformia animals, the highest prevalence was registered by culture in bobcats [37%, 7/19, (55)], by IFA in bobcats again [74%, (56)], and by PCR of blood in Iberian lynx [33.3%, 10/30, (57)]. In studies of over two Caniformia animals, the highest Bartonella prevalence was registered by culture in gray foxes [49%, 26/53, (58)], by IFA in coyotes [89%, 48/53, (58)], and by PCR of blood in raccoons [43%, 16/37, (59)].…”

Section: Prevalence Of Bartonella Infections In Wild Carnivoresmentioning

confidence: 99%

“…In a study on free-ranging mountain lions and bobcats from California, U.S., Chomel et al (55) described new Bartonella strains, which were similar to but different from B. henselae and B. koehlerae , and named them B. koehlerae subsp. boulouisii and B. koehlerae subsp.…”

Section: Bartonella Species Identified In Wild Carnivoresmentioning

confidence: 99%

“…bothieri . Phylogenetic analysis based on comparison of four genetic markers revealed two clusters: one with five strains obtained from bobcats and another with three strains obtained from mountain lions indicating a degree of host-speciation of these strains (55). In Brazil, sequencing analysis revealed a Bartonella strain close to but different from B. henselae and B. koehlerae in wild-born captive margay ( Leopardus wiedii ) (235).…”

Section: Bartonella Species Identified In Wild Carnivoresmentioning

confidence: 99%

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Comparative Ecology of Bartonella and Brucella Infections in Wild Carnivores

Kosoy

Goodrich

2019

Front. Vet. Sci.

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Phylogenetic sister clades Bartonella and Brucella within the order Rhizobiales present some common biological characteristics as well as evident differences in adaptations to their mammalian reservoirs. We reviewed published data on Bartonella and Brucella infections in wild carnivores to compare the ecology of these bacteria in relatively similar host environments. Arthropod vectors are the main mechanism for Bartonella species transmission between mammalian hosts. The role of arthropods in transmission of Brucella remains disputed, however experimental studies and reported detection of Brucella in arthropods indicate potential vector transmission. More commonly, transmission of Brucella occurs via contact exposure to infected animals or the environment contaminated with their discharges. Of 26 species of carnivores tested for both Bartonella and Brucella, 58% harbored either. Among them were bobcats, African lions, golden jackals, coyotes, wolves, foxes, striped skunks, sea otters, raccoons, and harbor seals. The most common species of Bartonella in wild carnivores was B. henselae, found in 23 species, followed by B. rochalimae in 12, B. clarridgeiae in ten, and B. vinsonii subsp. berkhoffii in seven. Among Brucella species, Br. abortus was reported in over 30 terrestrial carnivore species, followed by Br. canis in seven. Marine carnivores, such as seals and sea lions, can host Br. pinnipedialis. In contrast, there is no evidence of a Bartonella strain specific for marine mammals. Bartonella species are present practically in every sampled species of wild felids, but of 14 Brucella studies of felids, only five reported Brucella and those were limited to detection of antibodies. We found no reports of Bartonella in bears while Brucella was detected in these animals. There is evident host-specificity of Bartonella species in wild carnivores (e.g., B. henselae in felids and B. vinsonii subsp. berkhoffii in canids). A co-adaptation of Brucella with terrestrial wild carnivore hosts is not as straightforward as in domestic animals. Wild carnivores often carry the same pathogens as their domesticated relatives (cats and dogs), but the risk of exposure varies widely because of differences in biology, distribution, and historical interactions.

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“…In recent years, new isolated species have been continually added to Bartonella genus (2). It is reported that seventeen of these species and 3 subspecies are associated with human disease; ( [2] http://www.bacterio.net/ bartonella.htm) (3)(4)(5)(6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

Seropositivity of <i>Bartonella henselae</i> in Risky Human Population, Cats and Dogs

Aydın¹,

Korkmazgil²,

Kırkan³

et al. 2019

meandros

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Amaç: Bartonella türleri insanlarda kedi tırmığı hastalığı, basiller anjiomatozis, basiller peliozis, Carrion hastalığı, enfektif endokardit ve siper ateşi gibi pek çok hastalığa yol açmaktadır. Türkiye'de Bartonella'lara bağlı kedi tırmığı hastalığı ve basiller anjiomatozis olgu sunuları bulunmaktadır. Bu çalışmada, Türkiye'nin batısında, kedi/kopek sahibi riskli insan, kedi ve köpeklerdeki Bartonella henselae seropozitivitesinin araştırılması amaçlanmıştır. Gereç ve Yöntemler: Bu çalışmaya, 34'ü ev, 97'si sokak olmak üzere toplam 131 kedi/köpek sahibi ve 57'si ev kedisi, 48'i barınak kedisi olmak üzere toplam 105 kedi ile 45 ev köpeğinin serum örnekleri alınmıştır. Serum örneklerinde B. henselae IgG antikorları iki ticari kit kullanılarak immunfluoresan antikor yöntemi ile belirlenmiştir. Bulgular: B. henselae seropozitifliği, ev kedi/köpek sahiplerinde sokak kedi/köpek sahiplerine göre (sırasıyla %26,5 ve %6,8). Seropozitiflik, istatistiksel olarak anlamlı Objective: Bartonella species cause several diseases in humans such as cat scratch disease, bacillary angiomatosis, peliosis hepatis, endocarditis, Carrion disease and trench fever. There have been cat scratch disease and bacillary angiomatosis cases reports in Turkey. The aim of this study is to determine the seropositivity against Bartonella henselae in cat/dog owners who are in the risk group, cats and dogs in Western Aegean region, Turkey. MaterialsandMethods:In this study, B. henselae immunoglobulin (Ig) G positivity was investigated in a total of 281 samples including a total of 131 people, 34 of whom are pet cat/dog owners and 97 of whom are stray cat/dog owners; as well as a total of 105 cats, of which 57 pet cats, 48 shelter cats, and 45 pet dogs. Sera tested for the presence of antibodies against B. henselae IgG using immunofluorescence assay with two commercial kits. Results: B. henselae seropositivity of pet owners was significantly higher than the stray cat/dog owners (26.5% vs 6.8%). B. henselae IgG was found positive in 36.2% of total cats, 22.8% of pet cats, 52.1% of shelter cats. B. henselae seropositivity was found statistically higher in shelter cats than pet cats. No positivity was detected in the samples taken from the dogs. Conclusion: It is concluded that being pet owner at home poses a risk for B. henselae. For the differential diagnosis, especially in patients in close contact with cats, B. henselae infection should be considered.

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Isolation of Bartonella henselae and Two New Bartonella Subspecies, Bartonella koehlerae Subspecies boulouisii subsp. nov. and Bartonella koehlerae Subspecies bothieri subsp. nov. from Free-Ranging Californian Mountain Lions and Bobcats

Cited by 17 publications

References 44 publications

Bartonella DNA in heart tissues of bats in central and eastern Europe and a review of phylogenetic relations of bat-associated bartonellae

Bartonella DNA in heart tissues of bats in central and eastern Europe and a review of phylogenetic relations of bat-associated bartonellae

Comparative Ecology of Bartonella and Brucella Infections in Wild Carnivores

Seropositivity of <i>Bartonella henselae</i> in Risky Human Population, Cats and Dogs

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