Isolation of Lactoferrin and Its Concentration in Sows' Colostrum and Milk during a 21-Day Lactation2

Elliot, J. I.; Senft, B.; Erhardt, G.; Fraser, David R.

doi:10.2527/jas1984.5941080x

Cited by 35 publications

(24 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the LFC of the individual milk samples from the individual goats need to be determined so as to clarify the variation of LFC in goat's milk for establishing a suitable LFC threshold for grading the milk quality. In addition, the milk LFC during normal lactational period in human, cow and swine had been reported to be 1,000 to 2,000, 20 to 200 and 30 µg/ml, respectively [11,16,19,28]. Therefore, the milk LFC in goat during normal lactation is similar to that in cow.…”

Section: Discussionmentioning

confidence: 85%

See 1 more Smart Citation

Increase of Lactoferrin Concentration in Mastitic Goat Milk

Chen

Mao

2004

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. Lactoferrin concentration (LFC) in normal and mastitic milk of dairy goats were examined. LFC in bulk milk collected from 70 dairy goat farms and individual milk samples from 10 goats with mastitis were measured by enzyme-linked immunosorbent assay (ELISA) and their reaction time in methylene blue reduction test (MBRT) monitored. Bulk milk samples were categorized into three grades, such as high, normal and low, based on the reaction time in MBRT. The mean LFC in milk that was considered high quality (167 µg/ml) was significantly lower than that of those graded as normal (218 µg/ml) and low quality (304 µg/ml), while mean LFC in mastitic milk was 587 µg/ml. The correlation coefficient between milk LFC and MBRT time was found to be -0.7. Three goats were inoculated with Staphylococcus aureus into one of their udder halves. The mean milk LFC was found to be significantly higher (1500 µg/ml) than the control (30 µg/ml). These findings suggest that milk LFC may be useful as an index for intramammary infection in goats.

show abstract

Section: Discussionmentioning

confidence: 85%

“…Lactoferrin concentration (LFC) in human, cow and sow milk has been reported [11,16,19] but information on LFC in goat milk is scarce. Masson and Heremans [22] have reported the LFC in milk from guinea pig, cow, goat, mare and mouse by the radial immunodiffusion method.…”

mentioning

confidence: 99%

Increase of Lactoferrin Concentration in Mastitic Goat Milk

Chen

Mao

2004

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

show abstract

“…It appears that the goat, mouse and pig have a higher antimicrobial activity in the milk due to the spike of specific activities (i.e., lactoperoxidase in goat and lactoferrin in monogastric). However, despite the lack of change in expression of LTF from early to peak lactation (Figure 2), the amount of lactoferrin in milk of sows has been reported to peak during colostrogenesis and to decrease thereafter [104], while in the cow lactoferrin concentration in milk has been reported to both increase [105] or decrease [100] as lactation progress.…”

Section: Figurementioning

confidence: 97%

Milk Protein Synthesis in the Lactating Mammary Gland: Insights from Transcriptomics Analyses

Bionaz¹,

Hurley²,

Loor³

2012

Milk Protein

View full text Add to dashboard Cite

“…Notably, compared to other commonly used animal models, pigs have higher concentrations of milk Lf, ranging from 1.2 mg/ml in colostrum to 0.3 mg/ml in mature milk (Elliot et al 1984;Gislason et al 1993), making it a good model to investigate the role of milk Lf during infancy and to examine the response to milk feeding and weaning. We have previously shown by binding studies the existence of a receptor for Lf in both pig duodenum and jejunum (Gislason et al 1993;Gislason et al 1995).…”

Section: Introductionmentioning

confidence: 99%

Cloning of a pig homologue of the human lactoferrin receptor: Expression and localization during intestinal maturation in piglets

Liao

López

Shafizadeh

et al. 2007

Comparative Biochemistry and Physiology Part A: Molecular &

View full text Add to dashboard Cite

The presence of a small intestinal lactoferrin receptor (SI-LfR) has been suggested in the pig, but remains to be identified. LfR has been suggested to play a key role in the internalization of lactoferrin (Lf) and to facilitate absorption of iron bound to Lf. The aim of this study was to identify the pig SILfR cDNA, determine its mRNA and protein expression during different stages of intestinal development. The coding region of the pig LfR cDNA was cloned by PCR using conserved sequences among species. LfR mRNA expression and protein abundance were measured in proximal small intestine from piglets at 1 week (pre-weaning), 3 weeks (weaning) and 6 months (post-weaning) of age by quantitative (real-time) RT-PCR (Q-PCR) and Western blot, respectively. Intestinal brush border membrane vesicles (BBMV) were also isolated to examine LfR abundance on the apical membrane. We determined the pig SI-LfR open reading frame (ORF) consists of 972 bp, resulting in a protein with a molecular mass ~135 kD and ~35 kD under non-reducing and reducing conditions, respectively. Using Q-PCR, we determined LfR expression significantly increased with age in the duodenum and reciprocally decreased in the jejunum. Intestinal LfR protein expression was maintained at all timepoints in the jejunum; however, in the duodenum LfR abundance reached maximum levels at 6 months. In BBMV fractions, LfR abundance significantly increased with age. Taken together our findings demonstrate the presence of a human SI-LfR homologue in pig, with mRNA and protein expression concomitantly regulated in the duodenum and inversely regulated in the jejunum. These findings suggest a mechanism by which pig Lf can be internalized in the intestine.

show abstract

Isolation of Lactoferrin and Its Concentration in Sows' Colostrum and Milk during a 21-Day Lactation2

Cited by 35 publications

References 10 publications

Increase of Lactoferrin Concentration in Mastitic Goat Milk

Increase of Lactoferrin Concentration in Mastitic Goat Milk

Milk Protein Synthesis in the Lactating Mammary Gland: Insights from Transcriptomics Analyses

Cloning of a pig homologue of the human lactoferrin receptor: Expression and localization during intestinal maturation in piglets

Contact Info

Product

Resources

About