Isolation of Myogenic Stem Cells from Cultures of Cryopreserved Human Skeletal Muscle

Zheng, Bo; Chen, Chien-Wen; Li, Guangheng; Thompson, Seth D.; Poddar, Minakshi; Péault, Bruno; Huard, Johnny

doi:10.3727/096368912x636876

Cited by 26 publications

(28 citation statements)

References 19 publications

(47 reference statements)

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“…These results indicate that the cells’ phenotype is dynamic, and thus cell population purity after culture must be carefully considered when using tissue engineering or other therapeutic strategies for meniscus repair. We have observed similar loss of surface marker expression in CD34 and CD144 myogenic cells after long-term expansion (33,35). …”

Section: Discussionsupporting

confidence: 66%

The Use of Blood Vessel–Derived Stem Cells for Meniscal Regeneration and Repair

Osawa

Harner

Gharaibeh

et al. 2013

Medicine &Amp; Science in Sports &Amp; Exercise

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Purpose Surgical repairs of tears in the vascular region of the meniscus usually heal better than repairs performed in the avascular region; thus, we hypothesized that this region might possess a richer supply of vascular-derived stem cells than the avascular region. Methods In this study, we analyzed 6 menisci extracted from aborted human fetuses and 12 human lateral menisci extracted from adult human subjects undergoing total knee arthroplasty. Menisci were immunostained for CD34 (a stem cell marker) and CD146 (a pericyte marker) in situ, whereas other menisci were dissected into two regions (peripheral and inner) and used to isolate meniscus-derived cells by flow cytometry. Cell populations expressing CD34 and CD146 were tested for their multi-lineage differentiation potentials, including chondrogenic, osteogenic, and adipogenic lineages. Fetal peripheral meniscus cells were transplanted by intracapsular injection into the knee joints of an athymic rat meniscal tear model. Rat menisci were extracted and histologically evaluated after 4 wk posttransplantation. Results Immunohistochemistry and flow cytometric analyses demonstrated that a higher number of CD34- and CD146-positive cells were found in the peripheral region compared with the inner region. The CD34- and CD146-positive cells isolated from the vascular region of both fetal and adult menisci demonstrated multilineage differentiation capacities and were more potent than cells isolated from the inner (avascular) region. Fetal CD34- and CD146-positive cells transplanted into the athymic rat knee joint were recruited into the meniscal tear sites and contributed to meniscus repair. Conclusions The vascularized region of the meniscus contains more stem cells than the avascular region. These meniscal-derived stem cells were multi-potent and contributed to meniscal regeneration.

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Section: Discussionsupporting

confidence: 66%

The Use of Blood Vessel–Derived Stem Cells for Meniscal Regeneration and Repair

Osawa

Harner

Gharaibeh

et al. 2013

Medicine &Amp; Science in Sports &Amp; Exercise

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“…Alternatively, based on a modified set of selective cell surface markers, it is feasible to purify MECs and PCs from cryopreserved human primary skeletal muscle cell cultures by flow cytometry 30 . This method allows prospective purification of two hBVSC subsets from banked human skeletal muscle culture for therapeutic purposes.…”

Section: Discussionmentioning

confidence: 99%

Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle

Chen

Saparov

Corselli

et al. 2014

JoVE

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Since the discovery of mesenchymal stem/stromal cells (MSCs), the native identity and localization of MSCs have been obscured by their retrospective isolation in culture. Recently, using fluorescence-activated cell sorting (FACS), we and other researchers prospectively identified and purified three subpopulations of multipotent precursor cells associated with the vasculature of human skeletal muscle. These three cell populations: myogenic endothelial cells (MECs), pericytes (PCs), and adventitial cells (ACs), are localized respectively to the three structural layers of blood vessels: intima, media, and adventitia. All of these human blood-vessel-derived stem cell (hBVSC) populations not only express classic MSC markers but also possess mesodermal developmental potentials similar to typical MSCs. Previously, MECs, PCs, and ACs have been isolated through distinct protocols and subsequently characterized in separate studies. The current isolation protocol, through modifications to the isolation process and adjustments in the selective cell surface markers, allows us to simultaneously purify all three hBVSC subpopulations by FACS from a single human muscle biopsy. This new method will not only streamline the isolation of multiple BVSC subpopulations but also facilitate future clinical applications of hBVSCs for distinct therapeutic purposes.

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“…MECs not only express MC markers such as CD56 and Pax7, but also express EC markers including CD34, CD144 (VE-cadherin), von Willebrand factor (vWF), and Ulex europaeus agglutinin-1 (UEA-1) [107,110]. Though present at a very low frequency in situ , MECs (CD34+/CD56+/CD144+/CD45−) can be purified by FACS to homogeneity.…”

Section: Human Blood Vessel-derived Stem Cells For Cardiac Repair mentioning

confidence: 99%

The Role of Antioxidation and Immunomodulation in Postnatal Multipotent Stem Cell-Mediated Cardiac Repair

Saparov

Chen

Beckman

et al. 2013

IJMS

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Oxidative stress and inflammation play major roles in the pathogenesis of coronary heart disease including myocardial infarction (MI). The pathological progression following MI is very complex and involves a number of cell populations including cells localized within the heart, as well as cells recruited from the circulation and other tissues that participate in inflammatory and reparative processes. These cells, with their secretory factors, have pleiotropic effects that depend on the stage of inflammation and regeneration. Excessive inflammation leads to enlargement of the infarction site, pathological remodeling and eventually, heart dysfunction. Stem cell therapy represents a unique and innovative approach to ameliorate oxidative stress and inflammation caused by ischemic heart disease. Consequently, it is crucial to understand the crosstalk between stem cells and other cells involved in post-MI cardiac tissue repair, especially immune cells, in order to harness the beneficial effects of the immune response following MI and further improve stem cell-mediated cardiac regeneration. This paper reviews the recent findings on the role of antioxidation and immunomodulation in postnatal multipotent stem cell-mediated cardiac repair following ischemic heart disease, particularly acute MI and focuses specifically on mesenchymal, muscle and blood-vessel-derived stem cells due to their antioxidant and immunomodulatory properties.

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Isolation of Myogenic Stem Cells from Cultures of Cryopreserved Human Skeletal Muscle

Cited by 26 publications

References 19 publications

The Use of Blood Vessel–Derived Stem Cells for Meniscal Regeneration and Repair

The Use of Blood Vessel–Derived Stem Cells for Meniscal Regeneration and Repair

Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle

The Role of Antioxidation and Immunomodulation in Postnatal Multipotent Stem Cell-Mediated Cardiac Repair

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