Isozyme variation among four species of the catfish genus <i>Clarias</i>

Na-Nakorn, Uthairat; Sodsuk, Panom K.; Wongrat, P.; Janekitkarn, S.; Bartley, D. M.

doi:10.1111/j.1095-8649.2002.tb02428.x

Cited by 12 publications

(9 citation statements)

References 16 publications

(20 reference statements)

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“…Therefore, correct species labeling, morphological taxonomy and voucher documentation should be prioritized in case that reassessment of spurious data is necessary [23] . Mislabeling is not unexpected since both of these species are genetically homologous [24] and morphologically similar. It has been reported that C. macrocephalus could not be distinguished from a female C. batrachus [25] .…”

Section: Discussionmentioning

confidence: 91%

DNA Barcoding of Catfish: Species Authentication and Phylogenetic Assessment

et al. 2011

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As the global market for fisheries and aquaculture products expands, mislabeling of these products has become a growing concern in the food safety arena. Molecular species identification techniques hold the potential for rapid, accurate assessment of proper labeling. Here we developed and evaluated DNA barcodes for use in differentiating United States domestic and imported catfish species. First, we sequenced 651 base-pair barcodes from the cytochrome oxidase I (COI) gene from individuals of 9 species (and an Ictalurid hybrid) of domestic and imported catfish in accordance with standard DNA barcoding protocols. These included domestic Ictalurid catfish, and representative imported species from the families of Clariidae and Pangasiidae. Alignment of individual sequences from within a given species revealed highly consistent barcodes (98% similarity on average). These alignments allowed the development and analyses of consensus barcode sequences for each species and comparison with limited sequences in public databases (GenBank and Barcode of Life Data Systems). Validation tests carried out in blinded studies and with commercially purchased catfish samples (both frozen and fresh) revealed the reliability of DNA barcoding for differentiating between these catfish species. The developed protocols and consensus barcodes are valuable resources as increasing market and governmental scrutiny is placed on catfish and other fisheries and aquaculture products labeling in the United States.

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Section: Discussionmentioning

confidence: 91%

DNA Barcoding of Catfish: Species Authentication and Phylogenetic Assessment

et al. 2011

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“…AAAAAA 1 Genetic distance Nei's (1978) Genetic distance Nei's (1978) Nei's (1978) unbiased genetic distance based on mtDNA RFLP analysis of three Clarias species. Genetic distance/bootstrap value of each node indicated Agnese et al, 1997;Na-Nakorn et al, 1998, 2002. The observed heterozygosity values were comparable in samples of C. gariepinus from both India and Thailand (0.149 and 0.152, respectively), but higher than that observed for C. batrachus (0.05) and C. macrocephalus (0.08).…”

Section: Discussionmentioning

confidence: 69%

“…As against the values in the present study, the P and H for other African catfishes were: in Chrysichtys auratus (Geoffroy Saint-Hilaire) P varied from 0% to 10% and H ¼ 0.000-0.081 (Agnese, 1991); in Chrysichtys nigrodigitatus P ¼ 5-21% and H ¼ 0.017-0.080 (Agnese et al, 1989). Na-Nakorn et al (2002) estimated 15.4% polymorphic loci in C. gariepinus from Thailand with the observed heterozygosity (Hobs ¼ 0.055) and mean no. of alleles to be 1.2.…”

Section: Discussionmentioning

confidence: 77%

“…Moreover, the hybrid of C. gariepinus and C. macrocephalus is commercially cultured by >80% of farmers in Thailand (Na-Nakorn et al, 1998). Na-Nakorn et al (2002) reported that C. gariepinus could be easily distinguished as three Thai species of Clarias catfishes on the basis of allozymes. Sennan et al (2004) observed that wild C. macrocephalus introgressed with alleles of C. gariepinus (with allozyme and microsatellite loci) and concluded that inadvertent hybridization in farms and subsequent breeding of escapees with wild fish were responsible.…”

Section: Introductionmentioning

confidence: 99%

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Genetic identification of three species of the genus Clarias using allozyme and mitochondrial DNA markers

et al. 2007

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Genetic variability in three clariid species, Clarias batrachus, C. gariepinus and C. macrocephalus, were investigated by allozyme electrophoresis and mitochondrial DNA (ND 5/6) RFLP markers. The catfish species C. batrachus and C. macrocephalus are native to the Indian subcontinent and Southeast Asia, respectively, while the African catfish C. gariepinus was introduced to Asian countries for culture. Sixteen gene loci from 12 enzyme systems were analyzed. Fixed allelic differences were evident between pairs of species at least at four loci. In mtDNA RFLP analysis, eight composite haplotypes were observed and each species was characterized by a set of haplotypes. The UPGMA phylogenetic tree revealed three distinct clusters: C. batrachus; C. gariepinus (India and Thailand); and C. macrocephalus.

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“…They found that a combined approach using three assays could reliably distinguish these two species in this case. Na-Nakorn et al (2002) found that isozyme markers could be used to make clear distinctions among four Clarias species found in Thailand.…”

Section: Molecular Markers For Species Identification and Analysis Of...mentioning

confidence: 99%

Biology of selected Clarias catfish species used in aquaculture

Haymer

Khedkar

2022

Israeli Journal of Aquaculture - Bamidgeh

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Catfish are important organisms used extensively in aquaculture. For this review, we are focusing on specific species of catfish in the genus Clarias, primarily Clarias batrachus, C. gariepinus and C. macrocephalus, because of their widespread use in aquaculture and the considerable amount of information that has been amassed on different aspects of their biology. This review summarizes information available on topics ranging from the taxonomy and geographic distribution of these catfish to work done on them at the genetic, molecular biological and genomic levels. This information may be useful in future efforts to grow and expand their use in aquaculture.

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Isozyme variation among four species of the catfish genus Clarias

Cited by 12 publications

References 16 publications

DNA Barcoding of Catfish: Species Authentication and Phylogenetic Assessment

DNA Barcoding of Catfish: Species Authentication and Phylogenetic Assessment

Genetic identification of three species of the genus Clarias using allozyme and mitochondrial DNA markers

Biology of selected Clarias catfish species used in aquaculture

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