Keragaman Genetik Kacang Bogor (Vigna subterranea L. Verdc.) Berdasarkan Marka SSR (Simple Sequence Repeat)

Abstract

<em></em><em>ABSTRACT<br /><br />Bambara groundnut (Vigna subterranea L. Verdc.) is an important underutilized legume crop in Indonesia. The aim of this research is to study genetic diversity of bambara groundnut from Sukabumi and Sumedang, West Java, Indonesia. This study used 107 bambara groundnut accessions, which consisted of 57 accessions from Sukabumi and 50 accessions from Sumedang. We use five simple sequence repeat (SSR) to analyze the accessions. Totally nine alleles were detected, with a mean of 1.8 alleles per locus. Allelic and gene diversities were higher in Sumedang (1.8 alleles per locus and 0.119) than in Sukabumi population (1.4 alleles per locus and 0.020), respectively. We constructed a phylogenic tree by Neighbor-Joining analysis based on genetic distances (DA) and showed the tree divided bambara groundnut accessions into two broad groups according to the origin of samples (Sukabumi and Sumedang). Results from the phylogenic tree are in line with those from the population structure analysis.<br /> <br />Keywords: allele diversity, bambara groundnut, genetic distance, under utilized legume<br /><br /></em>

KARAKTER MORFOLOGI DAN HUBUNGAN KEKERABATAN GALUR-GALUR KACANG BOGOR (Vigna subterranea L. Verdc.) ASAL LANRAS SUKABUMI

“…Berdasarkan penelitian tersebut, kacang bogor memiliki bentuk biji bulat dan oval, sementara warna bijinya ungu tua, coklat muda, merah tua, merah terang, dan coklat tua. Menurut Illahi (2015), keragaman genetik kulit biji a b a b kacang bogor asal Sukabumi lebih rendah dibandingkan kacang bogor asal Sumedang. Kacang bogor asal Sukabumi memiliki warna kulit biji coklat dan hitam, sedangkan kacang bogor asal Sumedang memiliki kulit biji warna krem, coklat, hitam keunguan dan hitam.…”

Illegitimacy Testing of Elaeis guineensis Population Based on Simple Sequence Repeat Markers

“…Simple sequence repeat markers are developed by amplifying the targeted SSR sequences using PCR. Copyright © 2019 Universitas Brawijaya Many studies have shown the presence of a high allelic variation among different plant populations (Illahi, Wiendi, & Sudarsono, 2016;Larekeng, Purwito, Mattjik, & Sudarsono, 2018;Oktavia, Kuswanhadi, Dinarty, Widodo, & Sudarsono, 2017;Purwoko, Cartealy, Tajuddin, Dinarti, & Sudarsono, 2019;Sulistiyorini, Rubiyo, & Sudarsono, 2018). The SSR markers are used as powerful tools for supporting plant breeding of many perennial crops, including oil palm (Ajambang, Sudarsono, Asmono, & Toruan, 2012;Budiman, Apriyanto, Pancoro, & Sudarsono, 2019;Larekeng, Maskromo, Purwito, Matjik, & Sudarsono, 2015;Maskromo, Larekeng, Novarianto, & Sudarsono, 2017;Natawijaya et al, 2019;Tinche, Asmono, Dinarti, & Sudarsono, 2014).…”