Kinetische untersuchungen zur substratüberschuss-hemmung gelöster und trägerfixierter invertase

Besserdich, H.; Kahrig, E.; Krenz, R.; Kirstein, D.

doi:10.1016/0304-5102(77)80024-2

Cited by 11 publications

(2 citation statements)

References 8 publications

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“…For the purposes of the simulations here presented, having considered an aqueous solution of sucrose to be hydrolysed, according to literature reports [ Besserdich et al, ], for K M the value of 0.06 mol dm − 3 was taken, while the value of k 2 was calculated through the relationship given by Daniel and Danson []:

k_{2} = \frac{k_{B} T}{h} \cdot normalexp ((), - \frac{Δ G^{+}}{R T})

in which k B and h are, respectively, the Boltzmann and the Planck constants, R is the universal gas constant, T is the temperature, and Δ G + the Gibbs free energy variation associated with the right reaction in scheme 1, here Δ G + = 88 kJ mol − 1 . Finally, for [E], the value of 10 mg dm − 3 was assumed, according to the available literature information [ Vásquez‐Bahena et al ., ].…”

Section: Multiphysics Analysismentioning

confidence: 99%

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Design and optimization of a microwave irradiated and resonant continuous biochemical reactor

et al. 2016

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The design of a microwave irradiated enzyme (biochemical) reactor is here presented. It consists of a suitable higher‐order mode resonant cavity which contains several tubes where the solution containing reagents and products flow in the laminar flow regime. The process evolution under irradiation has then been simulated using the COMSOL multiphysics environment. As an example, the enzymatic hydrolysis reaction of sucrose has been considered. The results of the multiphysics simulation show that such a reactor can be used to effectively perform the reaction process in the optimal conditions for maximizing the reaction rate and preventing the enzyme deactivation by a precise knowledge of the temperature distribution and its strict control.

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k_{2} = \frac{k_{B} T}{h} \cdot normalexp ((), - \frac{Δ G^{+}}{R T})

Section: Multiphysics Analysismentioning

confidence: 99%

“…For the purposes of the simulations here presented, having considered an aqueous solution of sucrose to be hydrolysed, according to literature reports [Besserdich et al, 1977], for K M the value of 0.06 mol dm À 3 was taken, while the value of k 2 was calculated through the relationship given by Daniel and Danson [2013]:…”

Section: Radio Sciencementioning

confidence: 99%

Design and optimization of a microwave irradiated and resonant continuous biochemical reactor

et al. 2016

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Application of immobilized invertase to continuous hydrolysis of concentrated sucrose solutions

Monsan

Combes

1984

Biotech & Bioengineering

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Invertase immobilized onto corn grits was utilized in the hydrolysis of highly concentrated sucrose solutions producting liquid sugar solutions containing glucose and fructose. Comparisons of conversion efficiencies of this immobilized invertase in a continuous stirredtank reactor and a plug-flow reactor indicated that the plug-flow reactor has an higher efficiency. Continuous sucrose hydrolysis was then performed in 0.1- and 1-L tubular reactors. This tenforld scaling-up was achieved without any noticeable loss in efficiency. This process thus was scaled-up to a 17.6-L pilot reactor set in a cane sugar refinery. This reactor was fed with highly concentrated sucrose solutions [71% (w/w)] to produce invert sugar syrup with the desired inversion degree. It allows a productivity equal to 9.1 kg sucrose hydrolyzed/h in the case of a 69% (w/w) sucrose initial concentration with a 72% conversion rate.

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Molecular aspects of affinity chromatography

Mohr

Pommerening

Kühn

1980

J. polym. sci., C Polym. symp.

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Affinity chromatography is based on the well‐known specific interaction between biomacromolecules and between biomacromolecules and low‐molecular partners. To separate the one component (bioaffine component), the complementary bioaffinant has to be attached chemically to a solid‐phase support. This interaction, however, can be disturbed considerably by the linkage of one partner to the insoluble matrix. Some molecular aspects of this interaction discussed in a summarized presentation of our investigations are: (1) conformational state of immobilized biomacromolecules; (2) nonspecific interaction between matrix and bioaffine component; (3) steric factors; and (4) interaction between spacer and/or carrier and bioaffine components.

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Kinetische untersuchungen zur substratüberschuss-hemmung gelöster und trägerfixierter invertase

Cited by 11 publications

References 8 publications

Design and optimization of a microwave irradiated and resonant continuous biochemical reactor

Design and optimization of a microwave irradiated and resonant continuous biochemical reactor

Application of immobilized invertase to continuous hydrolysis of concentrated sucrose solutions

Molecular aspects of affinity chromatography

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