Label-free homogeneous electrochemical detection of MicroRNA based on target-induced anti-shielding against the catalytic activity of two-dimension nanozyme

Wu, Jiahui; Lv, Wenxin; Yang, Qiaoting; Li, Haiyin; Li, Feng

doi:10.1016/j.bios.2020.112707

Cited by 158 publications

(82 citation statements)

References 34 publications

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“…Specifically, since no nucleic acid amplification method was introduced in this method, the LOQs of this method were at the same level as DNA detection assay based on SP-ICP-MS methods [ 20 , 23 ], but higher than that of other SP-ICP-MS methods [ 26 , 27 ] which introduced nucleic acid amplification. Compared with electrochemical methods, the LOQs of the developed method was lower than the method reported in reference [ 31 , 32 ], but slightly higher than the work with nucleic acid amplification involved [ 33 ]. In addition, this method was a homogeneous detection method, which was simple to operate.…”

Section: Resultsmentioning

confidence: 80%

A homogeneous nucleic acid assay for simultaneous detection of SARS-CoV-2 and influenza A (H3N2) by single-particle inductively coupled plasma mass spectrometry

Chen

et al. 2021

Analytica Chimica Acta

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In recent years, single particle inductively coupled plasma mass spectrometry (SP-ICP-MS) has become a powerful tool for biological quantitative analysis. Homogeneous analysis method requires no separation and washing steps, which is suited for the analysis of highly infectious pathogens, so as to reduce the risk of infection during the operation. SARS-CoV-2 spreads all over the world, and its early infection symptoms are similar to influenza, which brings inconvenience to triage. Therefore, developing novel analytical method for simultaneous detection of multiple viral nucleic acids is essential. Taking the advantages of SP-ICP-MS and homogeneous analysis strategy, a SP-ICP-MS homogeneous nucleic acid assay by using gold nanoparticles (Au NPs) and silver nanoparticles (Ag NPs) probes was established for simultaneous sensitive analysis of SARS-CoV-2 and influenza A (H3N2). In the present of target SARS-CoV-2 or H3N2 nucleic acids, corresponding Au NPs or Ag NPs probes form larger aggregates, resulting in increased pulse signal intensity and reduced pulse signal frequency of the corresponding NPs in SP-ICP-MS measurement. In this assay, the reaction system of Au NPs and Ag NPs probes does not interfere with each other, and there was no separation and washing procedure, which facilitates operation, saves the analysis time, and improves the analysis efficiency. The linear range of this method is 5-1000 pmol L -1 , with low-level limits of quantification of target nucleic acid. The developed SP-ICP-MS simultaneous homogeneous detection method has a good potential for detecting nucleic acid, protein, cell and other biological samples by changing different modification sequences on the NPs probes.

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Section: Resultsmentioning

confidence: 80%

A homogeneous nucleic acid assay for simultaneous detection of SARS-CoV-2 and influenza A (H3N2) by single-particle inductively coupled plasma mass spectrometry

Chen

et al. 2021

Analytica Chimica Acta

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“…In addition, the 2D MnO2 nanoflakes exhibited unique responses to ssDNA. Because of ssDNA sensitivity and dual enzyme-like activity, a homogeneous electrochemical biosensor for 2D MnO2 nanoflake-based miRNAs was developed with an excellent linear range of 0.4-100 nM for detecting miRNA let-7a, a biomarker for cancer [100].…”

Section: Dna and Rna Sensorsmentioning

confidence: 99%

Technological advances in electrochemical biosensors for the detection of disease biomarkers

et al. 2021

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With an increasing focus on health in contemporary society, interest in the diagnosis, treatment, and prevention of diseases has grown rapidly. Accordingly, the demand for biosensors for the early diagnosis of disease is increasing. However, the measurement range of existing electrochemical sensors is relatively high, which is not suitable for early disease diagnosis, requiring the detection of small amounts of biocomponents. Various attempts have been made to overcome this and amplify the signal, including binding with various labeling molecules, such as DNA, enzymes, nanoparticles, and carbon materials. Efforts are also being made to increase the sensitivity of electrochemical sensors, and the combination of nanomaterials, materials, and biotechnology offers the potential to increase sensitivity in a variety of ways. Recent studies suggest that electrochemical sensors can be a powerful tool in providing comprehensive insights into the targeting and detection of disease-associated biomarkers. Significant advances in nanomaterial and biomolecule approaches for improved sensitivity have resulted in the development of electrochemical biosensors capable of detecting multiple biomarkers in real time in clinically relevant samples. In this review, we have discussed the recent studies on electrochemical sensors for detection of diseases such as diabetes, degenerative diseases, and cancer. Further, we have highlighted new technologies to improve sensitivity using various materials, including DNA, enzymes, nanoparticles, and carbon materials.

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“…These superior properties permit them on various sensing mechanisms, including optical sensation through chemiluminescence, surface plasmon resonance (SPR), and electrochemistry for a field-effect transistor (FET, voltage-current under gating control) by p-n junctions for detecting specific biomolecules and gases [54,58,59]. For instance, 2D Mn oxides-based materials exhibit a powerful catalytic property that has been employed as nanoenzymes for catalyzing the hydrolysis of biomolecules via peroxidase-like activity, thereby producing electrochemical/electrochemiluminescence signals as the detection readouts [60][61][62]. While metal oxides may play a more critical role in chemical catalysis and fuel cells, TMDs can be a significant part of sensor developments [63,64].…”

Section: Two-dimensional Nanocompositesmentioning

confidence: 99%

Recent Advances in Two-Dimensional Transition Metal Dichalcogenide Nanocomposites Biosensors for Virus Detection before and during COVID-19 Outbreak

et al. 2021

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The deadly Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) outbreak has become one of the most challenging pandemics in the last century. Clinical diagnosis reports a high infection rate within a large population and a rapid mutation rate upon every individual infection. The polymerase chain reaction has been a powerful and gold standard molecular diagnostic technique over the past few decades and hence a promising tool to detect the SARS-CoV-2 nucleic acid sequences. However, it can be costly and involved in complicated processes with a high demand for on-site tests. This pandemic emphasizes the critical need for designing cost-effective and fast diagnosis strategies to prevent a potential viral source by ultrasensitive and selective biosensors. Two-dimensional (2D) transition metal dichalcogenide (TMD) nanocomposites have been developed with unique physical and chemical properties crucial for building up nucleic acid and protein biosensors. In this review, we cover various types of 2D TMD biosensors available for virus detection via the mechanisms of photoluminescence/optical, field-effect transistor, surface plasmon resonance, and electrochemical signals. We summarize the current state-of-the-art applications of 2D TMD nanocomposite systems for sensing proteins/nucleic acid from different types of lethal viruses. Finally, we identify and discuss the advantages and limitations of TMD-based nanocomposites biosensors for viral recognition.

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Label-free homogeneous electrochemical detection of MicroRNA based on target-induced anti-shielding against the catalytic activity of two-dimension nanozyme

Cited by 158 publications

References 34 publications

A homogeneous nucleic acid assay for simultaneous detection of SARS-CoV-2 and influenza A (H3N2) by single-particle inductively coupled plasma mass spectrometry

A homogeneous nucleic acid assay for simultaneous detection of SARS-CoV-2 and influenza A (H3N2) by single-particle inductively coupled plasma mass spectrometry

Technological advances in electrochemical biosensors for the detection of disease biomarkers

Recent Advances in Two-Dimensional Transition Metal Dichalcogenide Nanocomposites Biosensors for Virus Detection before and during COVID-19 Outbreak

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