Lack of direct DNA damage in human blood leukocytes and lymphocytes after in vitro exposure to high power microwave pulses

Chemeris, N. K.; Gapeyev, A. B.; Сирота, Н. П.; Gudkova, Olga Yu.; Tankanag, Arina V.; Konovalov, I. V.; Buzoverya, M. E.; Suvorov, V. G.; Logunov, Vyacheslav A.

doi:10.1002/bem.20196

Cited by 17 publications

(3 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Vijayalaxmi et al (2006) suggested that the observations reported in the REFLEX studies may have resulted from small differences in the incidence of apoptosis/proliferating cells among the samples, rather than from induction of DNA damage. In contrast to the studies just described, most in vitro studies failed to detect any evidence of primary DNA damage in a variety of mammalian cell lines exposed to RF fields (Hook et al, 2004;Zeni et al, 2005;Stronati et al, 2006;Sakuma et al, 2006;Chemeris et al, 2006). Several recent studies also failed to observe increased DNA damage in a variety of cells/tissues harvested from animals exposed to a range of RF field frequencies and intensities (Aitken et al, 2005;Belyaev et al, 2006;Verschaeve et al, 2006).…”

Section: Genetic Toxicologymentioning

confidence: 84%

Recent Advances in Research on Radiofrequency Fields and Health: 2004–2007

Habash

Elwood

Krewski

et al. 2009

Journal of Toxicology and Environmental Health, Part B

View full text Add to dashboard Cite

The widespread use of wireless telecommunications devices, particularly mobile phones and wireless networks, has resulted in increased human exposure to radiofrequency (RF) fields. Although national and international agencies have established safety guidelines for exposure to RF fields, concerns remain about the potential for adverse health outcomes to occur in relation to RF field exposure. The extensive literature on RF fields and health was reviewed by a number of authorities, including the Royal Society of Canada (1999). This report is the third in a series of updates to the original report of the Royal Society of Canada, covering the period 2004-2007. In particular, the present study examined new data on (1) dosimetry and exposure assessment, (2) biological effects of RF fields such as enzyme induction, and (3) toxicological effects, including genotoxicity and carcinogenicity. Epidemiological studies of the potential health effects of RF exposure, particularly from mobile phones, were determined, along with human and animal studies of neurological and behavioural effects. Within the last 4 yrs investigators concluded that there is no clear evidence of adverse health effects associated with RF fields, although continued research is recommended to address specific areas of concern, including exposure to RF fields among children using mobile phones. The results of the ongoing 13-country World Health Organization INTERPHONE study of mobile phones may provide important new information on the potential cancer risks associated with mobile phone use.

show abstract

Section: Genetic Toxicologymentioning

confidence: 84%

Recent Advances in Research on Radiofrequency Fields and Health: 2004–2007

Habash

Elwood

Krewski

et al. 2009

Journal of Toxicology and Environmental Health, Part B

View full text Add to dashboard Cite

show abstract

“…However, partial studies hold a reverse idea that microwave radiation has little hereditary toxicity, for example long‐term exposure (2 h/day, 5 days/week for 2 years) of rats to 900 MHz GSM signal at 0.3 and 0.9 W/kg did not significantly affect DNA strand breaks in cells (Verschaeve et al., ). Chemeris' results were exactly the same, high‐power microwave under the given exposure conditions did not induce DNA strand breaks, alkali‐labile sites and incomplete excision repair sites of human whole‐blood leucocytes and isolated lymphocytes exposed to 8.8‐GHz field at SAR of 1.6 kW/kg for 40 min and 30 min, respectively (Chemeris et al., ). Further studies are needed to evaluate the genotoxic effects of an ordinary mobile phone radiation.…”

Section: Introductionmentioning

confidence: 81%

Effect of Mobile Phone Radiation on Cardiovascular Development of Chick Embryo

Wang

Zhang

et al. 2015

Anat. Histol. Embryol.

View full text Add to dashboard Cite

The biological effects on cardiovascular development of chicken embryos were examined after radiation exposure using mobile phone (900 MHz; specific absorption rate˜1.07 W/kg) intermittently 3 h per day during incubation. Samples were selected by morphological and histological methods. The results showed the rate of embryonic mortality and cardiac deformity increased significantly in exposed group (P < 0.05). No any histological pathological changes were observed on Day 5-7 (D5-D7) of incubation. A higher distribution of lipid droplets was unexpectedly present in myocardial tissue from the exposure groups on D10-D13. Soon afterwards, myofilament disruption, atrioventricular valve focal necrosis, mitochondria vacuolization and atrial natriuretic peptide (ANP) decrease appeared on D15-D21 of incubation. Comet assay data showed the haemocyte mean tail in the exposed group was significantly larger than that of the control (P < 0.01). The arterial vascular wall of exposed group was thicker (P < 0.05) than that of the control on D13, which was reversed to normal in later stages. Our findings suggest that long-term exposure of MPR may induce myocardium pathological changes, DNA damage and increased mortality; however, there was little effect on vascular development.

show abstract

“…Thus nervous tissue appeared to be sensitive to HPPM [Pakhomov and Doyle, ; Pakhomov et al, ,]. In cellular studies, Chemeris et al [] showed no effect of HPPM on deoxyribonucleic acid (DNA) integrity or cell morphology in erythrocytes and white blood cells exposed to multiple 8.8 GHz, 180‐ns pulses at 50 Hz.…”

Section: Introductionmentioning

confidence: 99%

Cellular effects of acute exposure to high peak power microwave systems: Morphology and toxicology

Ibey

Roth

Ledwig

et al. 2016

Bioelectromagnetics

View full text Add to dashboard Cite

Electric fields produced by advanced pulsed microwave transmitter technology now readily exceed the Institute of Electrical and Electronic Engineers (IEEE) C.95.1 peak E-field limit of 100 kV/m, highlighting a need for scientific validation of such a specific limit. Toward this goal, we exposed Jurkat Clone E-6 human lymphocyte preparations to 20 high peak power microwave (HPPM) pulses (120 ns duration) with a mean peak amplitude of 2.3 MV/m and standard deviation of 0.1 with the electric field at cells predicted to range from 0.46 to 2.7 MV/m, well in excess of current standard limit. We observed that membrane integrity and cell morphology remained unchanged 4 h after exposure and cell survival 24 h after exposure was not statistically different from sham exposure or control samples. Using flow cytometry to analyze membrane disruption and morphological changes per exposed cell, no changes were observed in HPPM-exposed samples. Current IEEE C95.1-2005 standards for pulsed radiofrequency exposure limits peak electric field to 100 kV/m for pulses shorter than 100 ms [IEEE (1995) PC95.1-Standard for Safety Levels with Respect to Human Exposure to Electric, Magnetic and Electromagnetic Fields, 0 Hz to 300 GHz, Institute of Electrical and Electronic Engineers: Piscataway, NJ, USA]. This may impose large exclusion zones that limit HPPM technology use. In this study, we offer evidence that maximum permissible exposure of 100 kV/m for peak electric field may be unnecessarily restrictive for HPPM devices. Bioelectromagnetics. 37:141-151, 2016. © 2016 Wiley Periodicals, Inc.

show abstract

Lack of direct DNA damage in human blood leukocytes and lymphocytes after in vitro exposure to high power microwave pulses

Cited by 17 publications

References 17 publications

Recent Advances in Research on Radiofrequency Fields and Health: 2004–2007

Recent Advances in Research on Radiofrequency Fields and Health: 2004–2007

Effect of Mobile Phone Radiation on Cardiovascular Development of Chick Embryo

Cellular effects of acute exposure to high peak power microwave systems: Morphology and toxicology

Contact Info

Product

Resources

About