Lack of sensorial innervation in the newborn female rats affects the activity of hypothalamic monoaminergic system and steroid hormone secretion during puberty

Quiróz, Ubaldo; Morales‐Ledesma, Leticia; Morán, Carolina; Hernández, Adán; Domı́nguez, Roberto

doi:10.1007/s12020-013-0055-3

Cited by 3 publications

(1 citation statement)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The subcutaneous injection of capsaicin to newborn female rats resulted in lower monoaminergic activity in the hypothalamus when rats reached 20 days of life [ 27 ]. Moreover, we have previously shown that in rats, treatment with capsaicin on the third day of life resulted in a decrease in serum estradiol and progesterone concentrations and that this result was not observed when the treatment with capsaicin was performed in new-born rats [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

In the pubertal rat, the regulation of ovarian function involves the synergic participation of the sensory and sympathetic innervations that arrive at the gonad

Morales‐Ledesma¹,

Hernández

Apolonio³

2015

Reprod Biol Endocrinol

Self Cite

View full text Add to dashboard Cite

BackgroundThe present study investigates sectioning the superior ovarian nerve (SON) in rats with functional sensorial denervation induced by capsaicin administration at birth and the effects on the establishment of puberty, ovulation, serum progesterone, and estradiol concentrations.MethodsThe animals were allotted randomly to one of the following experimental groups. Groups of 8–10 rats were injected at birth with capsaicin or vehicle, and on day 20 or 28 of life, they were submitted to a sham operation (SO). Other groups of 8–10 rats were injected at birth with capsaicin or vehicle, and on day 20 or 28 of life, they were submitted to the uni-or bilateral SON sectioning. The animals were killed at the first estrus. Serum concentration of progesterone (ng/ml) and estradiol (pg/ml) were measured using a radioimmunoassay.ResultsAnimals treated with capsaicin and subjected at 20 days of life to the left or bilateral section of SON had a delayed age of vaginal opening. Furthermore, animals with a lack of sensory information and subjected to a SO at 28 days of life had the same delay in the age of vaginal opening. Animals with sensorial innervation intact, subjected to unilateral section of the SON at 20 or 28 days of age, showed diminished ovulation rate and number of ova shed by the denervated ovary. In animals with sensorial denervation, the uni-or bilateral sectioning of the SON did not result in changes in ovulation. Progesterone and estradiol levels were different depending on the age of the animal in which the SON section was performed.ConclusionsBased on the present results, we suggest that sympathetic innervation regulates ovulation and the secretion of steroid hormones and that the sensory fibers modulate the sympathetic innervation action on ovarian functions.

show abstract

Section: Introductionmentioning

confidence: 99%

In the pubertal rat, the regulation of ovarian function involves the synergic participation of the sensory and sympathetic innervations that arrive at the gonad

Morales‐Ledesma¹,

Hernández

Apolonio³

2015

Reprod Biol Endocrinol

Self Cite

View full text Add to dashboard Cite

show abstract

Effects of capsaicin on ovarian granulosa cell proliferation and apoptosis

Guler

Zık

2018

Cell Tissue Res

View full text Add to dashboard Cite

Capsaicin is the pungent ingredient in red peppers. Due to the effects on the sensory nerve fibers, capsaicin has been used to treat pain and inflammation associated with a variety of diseases including rheumatoid arthritis and diabetic neuropathy, obesity, and cardiovascular and gastrointestinal conditions. Despite the extensive publications on different systems, the studies of the effects on the ovary are very limited. The present study was conducted to examine the possible proliferative and/or apoptotic effects of various doses of capsaicin on primarily derived granulosa cells. In accordance with this purpose, ovarian granulosa cells were exposed to different doses of capsaicin for 24 and 48 h. The proliferative effects of capsaicin were examined by immunocytochemistry, immunofluorescence, and western blot using an antibody against proliferating cell nuclear antigen (PCNA) and cell viability assay (MTT). The effects on apoptosis were determined by immunocytochemistry and immunofluorescence using antibodies against cleaved caspase-3 and cleaved poly (ADP-ribose) polymerase (PARP). We showed that the number of apoptotic cells increased in a capsaicin dose and time-dependent manners. We found that a low dose of CAP in 24 h administration was more effective on granulosa cell proliferation. Our results suggest that low-dose and short-term administration of CAP may have a positive effect on ovarian folliculogenesis.

show abstract

Plant molecules and their influence on health and female reproduction

Sirotkin

Kolesárová

2022

Environmental Contaminants and Medicinal Plants Action on Female Reproduction

View full text Add to dashboard Cite

Lack of sensorial innervation in the newborn female rats affects the activity of hypothalamic monoaminergic system and steroid hormone secretion during puberty

Cited by 3 publications

References 55 publications

In the pubertal rat, the regulation of ovarian function involves the synergic participation of the sensory and sympathetic innervations that arrive at the gonad

In the pubertal rat, the regulation of ovarian function involves the synergic participation of the sensory and sympathetic innervations that arrive at the gonad

Effects of capsaicin on ovarian granulosa cell proliferation and apoptosis

Plant molecules and their influence on health and female reproduction

Contact Info

Product

Resources

About