Lack of synergistic effects in tomato plants co‐infected with tomato severe rugose virus and tomato chlorosis virus

Abstract

The begomovirus Tomato severe rugose virus (ToSRV) and the crinivirus Tomato chlorosis virus (ToCV), in single and co‐infections, are very common in tomato crops in Brazil. Both viruses are transmitted by the whitefly Bemisia tabaciMEAM1 (biotype B). The objective of this study was to analyse the interaction between ToSRV and ToCV in tomato plants of cultivars Santa Clara and Kada. Plants at 15, 30 and 45 days after emergence were inoculated with 30 viruliferous B. tabaci per plant. The following treatments were compared: plants inoculated with ToSRV, ToCV, ToSRV + ToCV, and healthy (control). The interaction between these viruses was analysed by measuring the virus titre by qPCR and the fresh and dry weights of the aerial parts of the tomato plants. Based on two independent assays, no significant effects for co‐infection of ToSRV and ToCV on virus titres and plant development were observed compared to single infections. The dry weight of tomato plants of both cultivars infected with ToSRV, ToCV, or co‐infected did not differ significantly. However, the dry weight of Santa Clara tomato plants infected with ToSRV, ToCV and ToSRV + ToCV showed mean reductions of 21.5%, 25.5% and 32%, respectively, compared to healthy plants, and mean reductions for Kada were 31.7%, 37.5% and 38%, respectively.

Amplifier Hosts May Play an Essential Role in Tomato Begomovirus Epidemics in Brazil

“…Some amplicons were randomly selected, purified, and sent for nucleotide sequencing at Macrogen Inc. (Seoul, South Korea) to confirm the identity of the virus. In the first experiment, ToSRV was also detected by qPCR with the specific primers ToSRV-F 5 -GCAACCGCCTCTAGCACTTC-3 and ToSRV-R 5 -GACCTGGTCTCCCCAACAAGG-3 and protocols described by Bampi et al (2019).…”

Infecção natural de algumas plantas hospedeiras do tomato chlorosis virus, quantificação viral e importância como fontes de inóculo para >i<Bemisia tabaci>/i< MEAM1

“…Foram utilizados os oligonucleotídeos iniciadores (senso) TGGGACCGAGTACATTCCAAC-3), nas concentrações de μM. Esses primers amplificam um fragmento de 140 pb correspondente a parte do gene da proteína Heat-shock protein 70 (Hsp70h)(BAMPI et al, 2019). As condições de amplificação utilizadas incluíram um ciclo a 37°C por 15 min, seguido de um ciclo a 95°C por 10 min, 35 ciclos a 95°C por 10 s, 60°C por 30 s e 72°C por 30 s. A reações ocorreram no termociclador 7500 Fast (Applied Biosystems).…”