Lafoensia pacari A. St.-Hil.: Wound healing activity and mechanism of action of standardized hydroethanolic leaves extract

Pereira, Lucas Olivo Martins; Vilegas, Wagner; Tangerina, Marcelo Marucci Pereira; Arunachalam, Karuppusamy; Balogun, Sikiru Olaitan; Orlandi-Mattos, Paulo Eduardo; Colodel, Edson Moleta; Martins, Domingos Tabajara de Oliveira

doi:10.1016/j.jep.2018.02.038

Cited by 31 publications

(25 citation statements)

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“…SELp presented great antioxidant activity, with EC 50 of 7.08 ± 0.05 µg/mL, despite the EC 50 values found for the positive control (EC 50 of 0.7 ± 0.008 µg/mL or 4.11 µM for gallic acid). Also, Pereira et al [6] obtained L. pacari extract and demonstrated EC 50 value for the extract of 8.5 ± 0.1 µg/mL, corroborating with the present data.…”

Section: Resultssupporting

confidence: 92%

Enhanced Skin Permeation of Punicalagin after Topical Application of Pluronic Micelles or Vesicles Loaded with Lafoensia pacari Extract

et al. 2021

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Punicalagin, the principal ellagitannin of Lafoensia pacari leaves, has proven antioxidant activity, and standardized extracts of L. pacari can be topically used for skin aging management. We hypothesized that Pluronic nanomicelles or vesicles could solubilize sufficiently large amounts of the standardized extracts of L. pacari and provide chemical stability to punicalagin. The standardized extracts of L. pacari were obtained with an optimized extraction procedure, and the antioxidant activity was characterized. Formulations containing Pluronic at 25% and 35% were obtained with or without Span 80. They were characterized by average diameter, polydispersity index, punicalagin content, physicochemical stability, and rheology. A release and skin permeation study was carried out in vertical diffusion cells. The extraction procedure allowed quantifying high punicalagin content (i.e., 141.61 ± 3.87 mg/g). The standardized extracts of L. pacari showed antioxidant activity for all evaluated methods. Pluronic at 25 and Pluronic at 35 with standardized extracts of L. pacari showed an average diameter of about 25 nm. The addition of Span 80 significantly increased the mean diameter by 15-fold (p < 0.05), indicating the spontaneous formation of vesicles. Pluronic formulations significantly protected punicalagin from chemical degradation (p < 0.05). Pluronic at 25 formulations presented as free-flowing liquid-like systems, while Pluronic at 35 resulted in an increase of about 44-fold in |ƞ*|. The addition of Span 80 significantly reduced the Pluronic sol-gel transition temperature (p < 0.05), indicating the formation of vesicles. Formulations with Span 80 significantly enhanced punicalagin skin permeation compared to formulations without Span 80 (p < 0.05). Formulations with Span 80 were demonstrated to be the most promising formulations, as they allowed significant permeation of punicalagin (about 80 to 315 µg/cm2), which has been shown to have antioxidant activity.

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Section: Resultssupporting

confidence: 92%

Enhanced Skin Permeation of Punicalagin after Topical Application of Pluronic Micelles or Vesicles Loaded with Lafoensia pacari Extract

et al. 2021

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“…In the aloe genus, A. vera has shown higher wound healing activity than that achieved using other aloe species: Aloe ferox and Aloe marlothii (Fox et al, 2017). Apart from A. vera, other plants believed to possess woundhealing capability include the leaves of Lafoensia pacari A. St.-Hil, a native Brazilian tree (Pereira et al, 2018), Sanguisorba officinalis L. (Zhang et al, 2018), oat Avena sativa (Veerasubramanian et al, 2018), Artemisia khorassanica (Nowrozani and Ranjbary, 2018), and Roman chamomile Chamaemelum nobile L. (Kazemian et al, 2016).…”

Section: Wound Healingmentioning

confidence: 99%

Pharmaceutical applications of Aloe vera

Heng

Zulfakar

2018

Indonesian J. Pharm.

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Aloe vera has been used as folk medicine for a host of therapeutic indications of which the inner gel is the component extensively used and studied. Proponents of the use of this plant suggest that it is easily available, economical, and have fewer side effects compared to commercial drug compounds. However, the active constituents and their exact mechanisms have yet to be fully elucidated. This review focuses on the identification of the active constituents and their functional mechanism in the areas of anti-diabetic, anti-inflammatory, wound healing, and antibacterial. Preliminary evidence was found to support the antidiabetic effect which extended to the early stages of the disease with no adverse effects at the dosages used. Although topical application for its anti-inflammatory effect may be delayed and minute, oral administration has shown a significant response. However, too high a dose has been linked with an initial inflammatory reaction. Favorable response to the gel has also been demonstrated to support the use of A. vera in wound-healing and as an antibacterial agent, although limited to simple, uncomplicated wounds. Different active constituents such as acemannan and aloin have been suggested for the effects of the plant and this may be interpreted as synergism among different compounds rather than the action of a single compound. Until robust evidence is available, the plant should only be used as an adjunct to other wellestablished evidence-based treatment modalities.

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“…Ethnopharmacological studies describe L. pacari as wound-healer (Vila Verde et al 2003), being useful for treating ulcer, gastritis, healing and inflammation (Souza 2007;Cabral & Pasa 2009;Jesus et al 2009). Literature reports the presence of flavonoids (Santos et al 2000;Pereira et al 2018) and tannins (Sampaio et al 2011;Carneiro et al 2016;Pereira et al 2018) in L. pacari leaves. These polyphenols are highly relevant towards the biological activity of plants and have important medical and ecological purposes (Monteiro et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Investigation of wound healing activity of Lafoensia pacari (Lythraceae) leaves extract cultivated in Goiás state, Brazil

et al. 2020

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Lafoensia pacari has been used in traditional folk medicine in Brazilian Cerrado to treat wounds. It is important to develop studies that can clarify how the biological activity of L. pacari occurs. The aim of this study was to evaluate the healing activity of L. pacari leaves extract in an animal model. The extract was prepared from plants cultivated in Goiania-GO, Brazil. The healing activity was assayed using cutaneous wound model in rats, and macroscopic, morphometric and histological analysis of wounds were also conducted. The presence of hydrolysable tannins was detected in thin layer chromatography and in high performance liquid chromatography analysis, may be suggested the presence of ellagitannins. In the evaluation of cutaneous wounds in rats it was possible to observe that the treatment with 10% (w/v) L. pacari extract provided a reduction in the time of cutaneous wound healing, with a significant increase in variables involved in healing, such as the number of blood vessels and collagen production. Therefore, this study shows that L. pacari wound healing potential may be related to the presence of ellagitannins and corroborates to ethnopharmacological reports regarding this plant.

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Lafoensia pacari A. St.-Hil.: Wound healing activity and mechanism of action of standardized hydroethanolic leaves extract

Cited by 31 publications

References 50 publications

Enhanced Skin Permeation of Punicalagin after Topical Application of Pluronic Micelles or Vesicles Loaded with Lafoensia pacari Extract

Enhanced Skin Permeation of Punicalagin after Topical Application of Pluronic Micelles or Vesicles Loaded with Lafoensia pacari Extract

Pharmaceutical applications of Aloe vera

Investigation of wound healing activity of Lafoensia pacari (Lythraceae) leaves extract cultivated in Goiás state, Brazil

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