Langmuir-Blodgett films and black lipid membranes in biospecific surface-selective sensors

Thermodynamic characteristics and Langmuir–Blodgett deposition behavior of mixed DPPA/DPPC monolayers at air/liquid interfaces

“…However, previous researchers have found a difficulty in transferring a phospholipid monolayer from the air/liquid interface onto a solid support to build bilayer or multilayer LB films [12][13][14][15][16]. This difficulty is very likely caused by the stronger affinity of phospholipid head groups to the water subphase than that to the substrate surface.…”

The Diverse Range of Possible Cell Membrane Interactions with Substrates: Drug Delivery, Interfaces and Mobility

“…Diffusion coefficients (D) are determined from the transit time ( ) of fluorescent tagged molecules in a very small observation volume (less than 10 −15 L) using a confocal setup. Many studies have measured the physical properties of the domains in the biological membranes by focusing on the development of artificial bio-membranes using either supported lipid bilayers (SLB) with Langmuir-Blodgett films or a vesicle disruption approach [ 112 , 113 ]. These protocols are mostly based on bottom-up techniques that frequently require multiple steps for each process, such as solvent evaporation, the optimization of the molar ratio between lipids and proteins for the formation of the domains, and the optimization of ionic strength and/or size; they also required an extremely clean environment.…”

Thin Films, Monomolecular Layers

“…The search for microbiosensors has brought the need for highly selective and highly sensitive organic layers, with tailored biological properties that can be incorporated into electronic, optical, or electrochemical devices (97). From the materials point of view, LB films are an excellent choice because of the high control on their chemical structure at the molecular level, and the ability to incorporate into them large biomolecules molecules.…”