Laser‐induced fluorescence emission: I. The spectroscopic identification of fibrotic endocardium and myocardium

Perk, Masis; Flynn, Gregory J.; Smith, Carolyn J.; Bathgate, Ben; Tulip, J.; Wen, Yurong; Lucas, Alexandra

doi:10.1002/lsm.1900110606

Cited by 14 publications

(2 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The diagnosis of arterial plaque is important for the application of laser angioplasty, and LIFS has been tested for guidance of laser ablation in vitro and in vivo (Papazoglou 1995, Deckelbaum et al 1989. Related to the detection of atherosclerotic plaque are investigations of LIFS for the identification of fibrotic endocardium and myocardium, and sinoatrial and atrioventricular nodal conduction tissue for the treatment of arrhythmia (Perk et al 1991(Perk et al , 1993. Also, work in the area of monitoring heart electrical activity using voltage sensitive dyes began in the early 1980s (Nassif et al 1985, Dillon andMorad 1981).…”

Section: Fluorescence Spectroscopymentioning

confidence: 99%

Ultraviolet and visible spectroscopies for tissue diagnostics: fluorescence spectroscopy and elastic-scattering spectroscopy

Bigio

Mourant²

1997

Phys. Med. Biol.

336

193

View full text Add to dashboard Cite

show abstract

Section: Fluorescence Spectroscopymentioning

confidence: 99%

Ultraviolet and visible spectroscopies for tissue diagnostics: fluorescence spectroscopy and elastic-scattering spectroscopy

Bigio

Mourant²

1997

Phys. Med. Biol.

336

193

View full text Add to dashboard Cite

show abstract

“…The various imaging techniques for extracting diagnostic information suggested in the literature, from visual examination following UV excitation to advanced multispectral imaging, are included. Primary fields of application of in vivo fluorometry include tissue metabolic studies (Tamura et al 1989, Horvath et al 1994, Cordeiro et al 1995, cardiovascular diagnosis (Perk et al 1991, Deckelbaum 1994, Deckelbaum et al 1995, Papazoglou 1995, Warren et al 1995, ophthalmology (Docchio 1989) and oncology. Most of the published in vivo fluorescence imaging studies deal with oncological applications; to identify early malignant lesions, for defining tumour extent and spread to adjacent tissues and as a guide for optimizing localized treatments of solid tumours.…”

Section: Introduction and Principlesmentioning

confidence: 99%

In vivofluorescence imaging for tissue diagnostics

et al. 1997

View full text Add to dashboard Cite

In vivo fluorescence imaging for tissue diagnosticsAndersson-Engels, Stefan; af Klinteberg, C; Svanberg, Katarina; Svanberg, Sune General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research.• You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal Take down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Abstract. Non-invasive fluorescence imaging has the potential to provide in vivo diagnostic information for many clinical specialities. Techniques have been developed over the years for simple ocular observations following UV excitation to sophisticated spectroscopic imaging using advanced equipment. Much of the impetus for research on fluorescence imaging for tissue diagnostics has come from parallel developments in photodynamic therapy of malignant lesions with fluorescent photosensitizers. However, the fluorescence of endogenous molecules (tissue autofluorescence) also plays an important role in most applications. In this paper, the possibilities of imaging tissues using fluorescence spectroscopy as a mean of tissue characterization are discussed. The various imaging techniques for extracting diagnostic information suggested in the literature are reviewed. The development of exogenous fluorophores for this purpose is also presented. Finally, the present status of clinical evaluation and future directions are discussed. In vivo fluorescence imaging for tissue diagnostics

show abstract

Fluorospectral study of the rat brain and glioma in vivo

Tsai¹,

Kao²,

Hsiao³

1993

Lasers Surg Med

View full text Add to dashboard Cite

An animal model of cerebral glioma was utilized by implanting C6 glioma cells into the brains of adult Wistar rats. Once tumors developed to 7-12 mm in diameter, we conducted continuous fluorimetry monitoring of glioma up to 24 hours using a fibre-optic system connected to an intensified multichannel photodetector after an intravenous injection of hematoporphyrin derivative (HPD) into the rats. The intensity of the fluorescence in normal brain reached a plateau 6 hours after intravenous injection of HPD while that in glioma reached a plateau 80 minutes after injection. These fluorescence intensities of glioma, brain adjacent to tumor (BAT), and surrounding normal brain were measured in vivo 24 hours after intravenous administration of 5 mg/kg of HPD. The ratio of fluorescence intensities between glioma and brain was 6.1 while the ratio between BAT and brain was 3.9. There were no obvious differences in shapes between the spectra of the natural fluorescence (autofluorescence) of rat glioma and brain but the intensity of autofluorescence was much weaker in glioma. There are many problems in spectroscopic studies of biological tissues in vivo. It cannot be overemphasized that very strict criteria must be applied in order to get accurate data. Fluorescence from HPD administration may be used to discriminate tumor tissue from surrounding normal brain tissue during operation if the measuring conditions could be kept constant. It is important to understand the photospectral properties of glioma and brain tissue in order to get the most benefits in clinical application of light-induced fluorescence or photoradiation therapy.

show abstract

Laser‐induced fluorescence emission: I. The spectroscopic identification of fibrotic endocardium and myocardium

Abstract: Fluorescence emission spectroscopy can differentiate normal and fibrotic endocardium and myocardium, in vitro. This technique may be useful for guidance during laser ablation of arrhythmogenic ventricular scar.

Cited by 14 publications

References 23 publications

Ultraviolet and visible spectroscopies for tissue diagnostics: fluorescence spectroscopy and elastic-scattering spectroscopy

Ultraviolet and visible spectroscopies for tissue diagnostics: fluorescence spectroscopy and elastic-scattering spectroscopy

In vivofluorescence imaging for tissue diagnostics

Fluorospectral study of the rat brain and glioma in vivo

Contact Info

Product

Resources

About