LC Determination of Lidocaine and Prilocaine Containing Potential Risky Impurities and Application to Pharmaceuticals

Mohammad, Mohammad Abdul‐Azim

doi:10.1365/s10337-009-1274-x

Cited by 6 publications

(9 citation statements)

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“…Of note is the value of resolution between the analytes, which was 8.5‐fold greater than the level considered suitable for chromatographic methods. In addition, this value was greater than the values described in a previous publication (Mohammad, ). The results for the other parameters related to the resolution, tailing factor and number of plates, were more adequate than those presented in other studies (Padula et al ., ; Mohammad, ).…”

Section: Resultscontrasting

confidence: 73%

“…In addition, this value was greater than the values described in a previous publication (Mohammad, ). The results for the other parameters related to the resolution, tailing factor and number of plates, were more adequate than those presented in other studies (Padula et al ., ; Mohammad, ). These findings proved that the column and the set of chromatographic conditions were successfully selected.…”

Section: Resultscontrasting

confidence: 73%

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A simple and high‐resolution HPLC‐PDA method for simultaneous quantification of local anesthetics in in vitro buccal permeation enhancement studies

Couto

Cubayachi

Lopez

et al. 2015

Biomedical Chromatography

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A simple, isocratic, high-resolution and prompt HPLC-PDA method was developed and validated for the simultaneous quantification of prilocaine (PCL) and lidocaine (LCL) hydrochlorides in in vitro buccal iontophoresis-driven permeation studies. A reversed-phase C18 column (250 mm x 4.6 mm, 3μm, 110Å) was used for the chromatographic separation. The mobile phase contained acetonitrile: 0.1M sodium phosphate buffer, pH 7.0 (1:1, v/v), plus 0.05% (v/v) diethylamine. The isocratic flow rate was set at 1 mL/min and the detection wavelength was 203 nm. PCL and LCL eluted in 8.9 min and 13 min, respectively, and the system suitability parameters varied within an acceptable range. The method was selective, sensitive, precise, accurate and robust, producing a linear plot at the concentration range of 0.25 to 10 µg/mL. The application of this method was demonstrated by a significant enhancement of the permeation of PCL and LCL with the application of iontophoresis (1 mA/cm(2) per 1 h) through isolated porcine esophageal epithelium. The amount of the drug retained in the epithelium also increased with the application of an electrical current. Copyright © 2015 John Wiley & Sons, Ltd.

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Section: Resultscontrasting

confidence: 73%

Section: Resultscontrasting

confidence: 73%

A simple and high‐resolution HPLC‐PDA method for simultaneous quantification of local anesthetics in in vitro buccal permeation enhancement studies

Couto

Cubayachi

Lopez

et al. 2015

Biomedical Chromatography

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“…The proposed method demonstrated to be very sensitive, as indicated by the low values (below 1 mg L −1 ) of the LOQ obtained. These LOQs were lower or comparable to those reported in previous works using CE , HPLC , and electrochemical detection . The intra‐day precision was assessed by analyzing in triplicate (in the same day) six different samples of pharmaceutical formulations (three samples containing LD and other three with BZ).…”

Section: Resultssupporting

confidence: 60%

“…In addition to HPLC methods of analysis for determination of LD and BZ in pharmaceutical formulations , analytical methods based on electrochemical and spectrophotometric detections have also been reported in the literature. Beyond quantification of LD in pharmaceutical formulations , CE has also been applied for the determination of this anesthetic in urine , human plasma , human serum , vitreous humor , and seized cocaine .…”

Section: Introductionmentioning

confidence: 99%

A simple and fast method for determination of benzocaine and lidocaine in pharmaceutical formulations by capillary electrophoresis with spectrophotometric detection

Junger

Jesus

Silva

et al. 2019

Separation Science Plus

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A novel, simple, and rapid method for the determination of the anesthetics benzocaine and lidocaine in pharmaceutical formulations using capillary electrophoresis with spectrophotometric detection (210 nm) is proposed. In this method, the sample preparation was fast and simple, using only aqueous dilution or extraction with sonication aid. A background electrolyte composed by 10 mmol L ‐1 of monosodium phosphate with pH 2.1 allowed the separation of the anesthetics with good peak resolution in less than 5 min. The limits of quantification were 0.31 and 0.68 mg L−1 for lidocaine and benzocaine, respectively. The intra‐ and inter‐day precisions (RSD%) ranged from 1.1 to 4.8% and the accuracies (as recovery percentage) varied from 84 to 109.4%. The proposed method was successfully applied for analyses of commercial samples of pharmaceutical formulations, such as otologic solution, ointment, and spray solution and lozenges for sore throat.

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“…No method for simultaneous analysis of these compounds in raw materials and veterinary pharmaceutical formulations has been reported. In addition, many HPLC procedures for analysis of LID in pharmaceutical preparations and biological fluids have been published [42] , [43] , [44] , as have the use of electrochemical methods for the direct quantification of LID and its impurities in pharmaceutical samples [42] , [43] , [44] , [45] , [46] .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of physicochemical properties as supporting information on quality control of raw materials and veterinary pharmaceutical formulations

Anacleto

Borges

Oliveira

et al. 2018

Journal of Pharmaceutical Analysis

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This study aimed to show that the physicochemical proprieties obtained by Fourier transform infrared spectroscopy (FTIR), thermogravimetry (TG), and scanning electronic microscopy (SEM) can be useful tools for evaluating the quality of active pharmaceutical ingredients (APIs) and pharmaceutical products. In addition, a simple, sensitive, and efficient method employing HPLC-DAD was developed for simultaneous determination of lidocaine (LID), ciprofloxacin (CFX) and enrofloxacin (EFX) in raw materials and in veterinary pharmaceutical formulations. Compounds were separated using a Gemini C18 (250 mm × 4.6 mm, 5 µm) Phenomenex® column, at a temperature of 25 °C, with a mobile phase containing 10 mM of phosphoric acid (pH 3.29): acetonitrile (85.7:14.3, v/v) and a flow rate of 1.5 mL/min. Physicochemical characterization by TG, FTIR, and SEM of raw materials of LID, CFX, and EFX provided information useful for the evaluation, differentiation, and qualification of raw materials. Finally, the HPLC method was proved to be useful for evaluation of raw material and finished products, besides satisfying the need for an analytical method that allows simultaneous determination of EFX, CFX, and LID, which can also be extended to other matrices and applications.

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LC Determination of Lidocaine and Prilocaine Containing Potential Risky Impurities and Application to Pharmaceuticals

Abstract: Unfortunately, in Fig. 1 of the original article the assignments of peaks 4 and 5 should be exchanged. The correct figure is given below. The online version of the original article can be found under

Cited by 6 publications

References 0 publications

A simple and high‐resolution HPLC‐PDA method for simultaneous quantification of local anesthetics in in vitro buccal permeation enhancement studies

A simple and high‐resolution HPLC‐PDA method for simultaneous quantification of local anesthetics in in vitro buccal permeation enhancement studies

A simple and fast method for determination of benzocaine and lidocaine in pharmaceutical formulations by capillary electrophoresis with spectrophotometric detection

Evaluation of physicochemical properties as supporting information on quality control of raw materials and veterinary pharmaceutical formulations

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