LINC00978 promotes bladder cancer cell proliferation, migration and invasion by sponging miR‑4288

Abstract

long noncoding rnas regulate the occurrence and progression of numerous types of cancer, including bladder cancer (Bca). However, the role of long intergenic non-protein coding rna 978 (linc00978) in Bca remains unknown. In the present study, it was identified that the LINC00978 expression level was significantly increased in BCa tissues compared with adjacent normal tissues. By performing cell proliferation, colony formation, Transwell and Matrigel assays, it was demonstrated that LINC00978 knockdown significantly inhibited the proliferation, migration and invasion of Bca cells. conversely, overexpression of linc00978 resulted in opposite effects. Mechanistically, it was demonstrated that linc00978 served as a competing endogenous rna to sponge microrna-4288 (mir-4288), and linc00978 knockdown significantly increased the expression level of miR-4288 in BCa cells. Furthermore, it was demonstrated that the expression of linc00978 was inversely correlated with the mir-4288 expression level in Bca tissues. Furthermore, by performing a rescue assay, it was identified that inhibition of miR-4288 was able to reverse the effects of linc00978 knockdown on proliferation, migration and invasion of Bca cells. collectively, the present study demonstrated that linc00978 promoted Bca progression by sponging mir-4288, suggesting that linc00978 may represent a potential therapeutic target for Bca treatment.

MIR4435-2HG Is a Potential Pan-Cancer Biomarker for Diagnosis and Prognosis

“…In tumors of the urinary system, MIR4435-2HG is abnormally upregulated in cancer tissues and cancer cell lines of clear cell renal cell carcinoma and bladder cancer ( 3 , 40 , 41 ). In nervous system tumors, MIR4435-2HG is highly expressed in plasma, cancer tissues and cancer cell lines of glioma ( 42 , 43 ), and in cancer tissues and cancer cell lines of glioblastoma ( Figure 3 ) ( 44 ).…”

“…Here, this article outlines the ceRNA network centered on MIR4435-2HG and its biological significance ( Figure 6 ). MIR4435-2HG can be used as the ceRNA of 20 miRNAs, 19 of which are found in 14 cancers, including miR-22-3p ( 18 ), miR-206-3 ( 1 ), miR-296-5p ( 1 ), miR-497-5p ( 11 ), miR-138-5p ( 53 ), miR-203a-3p ( 13 ), miR-6754-5p ( 25 ), miR-204-5p ( 26 ), miR-528 ( 30 ), miR-202 ( 30 ), miR-125a-5p ( 52 ), miR125b-5p ( 52 ), miR-1224-5p ( 44 ), miR-4288-3p ( 41 ), miR-513a-5p ( 3 ), miR-128- 3p ( 33 ), miR-93-3p ( 45 ), miR-802-5p ( 48 ), and miR-383-5p ( 47 ). In addition, in osteoarthritis, MIR4435-2HG was found to sponge miR-510-3p ( 63 ).…”

“…In glioblastoma, MIR4435-2HG competitively binds miR-1224-5p, thereby up-regulating TGFBR2 and promoting tumor growth ( 44 ). In bladder cancer, MIR4435-2HG can sponge miR-4288-3p and promote tumor growth and invasion ( 41 ). In clear cell renal cell carcinoma, MIR4435-2HG/miR-513a-5p promotes tumorigenesis and development by promoting the expression of KLF6 ( 3 ).…”

LncRNA00978 contributes to growth and metastasis of hepatocellular carcinoma cells via mediating microRNA-125b-5p/SOX12 pathway

“…Recently, accumulating biomarkers are identified for early detection and survival prediction in HCC [ 22–24 ]. Previous studies have suggested that LINC00978 is an oncogenic LncRNA in diverse cancers, like breast cancer [ 25 ], melanoma [ 26 ] and bladder cancer [ 27 ]. Our data presented that LINC00978 was upregulated in HCC.…”

“…Apart from mediating DNA, RNA, or proteins, LncRNAs also served as a ceRNA of miRNA [ 27 ]. For instance, LINC00978 could facilitate the progression of breast carcinoma via working as a sponger for microRNA-4288.…”