Lipase-catalyzed biosynthesis of cinnamoylated lipids in a selected organic solvent medium

Lipase-catalyzed Hydrolysis of Linseed Oil : Optimization Using Response Surface Methodology

“…With the increasing of enzyme load, the hydrolysis ratio linearly increased and then tend to be stable. Due to the accumulation of glycerol molecules in the reaction system, the hydrophilic hindrance layer was formed, which limited the substrate diffusion 29,30 . Figure 3 d incicates the mutual interaction between temperature and reaction time.…”

Synthesis and Characterization of Phenolic Lipids

“…Mellou et al [112] found that the conversion yield of rutin during esteriication reaction with oleic acid catalyzed by immobilized C. antarctica lipase B in diferent solvents was varied from 37 to 71% under the use of molecular sieves (100 mg/ ml). However, Karboune et al [113] observed 28 and 35% decrease in the maximum conversion yield upon the addition of 10 mg/ml of molecular sieves to the lipase-catalyzed biosynthesis of cinnamoylated lipids. This could be explained by the fact that molecular sieves promote the lipase-catalyzed synthesis reactions by dehydrating; however, excess of molecular sieves will capture the necessary water of enzyme, which may inhibit the enzyme activity.…”

Lipase-catalyzed acidolysis of fish liver oil with dihydroxyphenylacetic acid in organic solvent media

“…The enzymatic processes can be used in the production of fats and oils containing beneficial fatty acids and or/phenolic compounds Karboune et al, 2005;Sabally et al, 2006a,b and2007).…”

“…In agreement to Stamatis et al (1999) recent work in our laboratory resulted in a successful 100% esterification yield of cinnamic acid and oleyl alcohol using different solvent mixtures and substrate molar ratios . In addition to recent work in our laboratory, Karboune et al (2005) conducted work on the transesterification of cinnamic acid with triolein in organic solvent media. A combined yield of monoleyl-1(3)-cinnamate and dioleyl-2-cinnamate of 19% was obtained in an equal ratio of cinnamic and triolein ratio; however using a 4.5 fold excess triolein, the yield increased to 42% of combined monoleyl-1(3)-cinnamate and dioleyl-2-cinnamate.…”

“…Zaks and Kilbanov (1988) reported that the addition of molecular sieves increased the rate and bioconversion level; this is due to the effect of the molecular sieves to sequester the water layer from the enzyme molecule which is essential for the water-enzyme interaction (Halling, 2000). However, Karboune et al (2005) observed a 28 and 35% decrease in the maximum bioconversion yield upon the addition of 10 mg/mL of molecular sieves to the lipase-catalyzed biosynthesis of cinnamoylated lipids.…”