Lipid Peroxidation and Protein Oxidation in Azotobacter Vinelandii Exposed to Mercury, Silver, Crude Oil, and Fenton Reagent

Abstract

Pollu t ion Con t r ol & Biot ech n ology Un it , Depa rt m en t of Bioch em ist ry, U n iver sit y of Niger ia , Nsu kk a , E n u gu St a t e, Niger iaReceived 11 Novem ber 1998; a ccep ted 1 J u ly 1999. The a u t h or wish es t o t h a n k Ch ief Tech n ologist E n gr. J. U. Or a egbu n a a n d J. C. N wok edi for in st r u m en t a t ion , Mr s. C. O. Sibeu du of t h e Depa r t m en t of Pet r oleu m Resou r ces, Niger ia n N a t ion a l Pet r oleu m Cor por a t ion , Mosco Roa d, Por t H a r cou r t, for a ssist a n ce in secu r ing t h e Bon n y light cr u de oil a n d r eleva n t liter a t u r e, a n d Ok or on k wo Ka n u (MNI A), t h e pr incipa l a r ch it ect , Design Modu les, E n u gu , Niger ia, for e-m a il fa cility. Th is wor k wa s su p por t ed in pa r t by t h e Un iver sit y of Niger ia Sen a t e Resea r ch Gra n t No 93/101.Addr ess cor r es pon den ce t o I. N. E. On wu r a h , Pollu t ion Con t r ol & Biot ech n ology Un it , Dept . of Bioch em istr y, Un iver sit y of Niger ia , Nsu k ka , E n u gu St a t e, Niger ia.

Effects of ingested crude oil contaminated diets on antioxidant enzyme and lipid profile in Wistar albino rat

“…There was significant increase observed in the 1 st and 2 nd week of exposure. The result agrees with the work of Ledig et al, (1988), who reported an increase in the activity of SOD in the erythrocyte of alcoholic patients and microorganisms exposed to crude oil and fenton reagent (Onwurah, 1999). But, a prolonged exposure to the 3 rd week decreased the activity of the enzyme.…”

“…This was also observed in group 2 (1%) except in week 3 when the increase was substantially high (P<0.05). The pattern of increase also observed (Reid et al, 1994), fenton reagent and crude oil (Onwurah, 1999). It has been reported that high lipid peroxidation products observed in liver and brain homogenates may be as a result of damage to the cell membrane caused by free radical attack (Onwurah, 1999).…”

“…The pattern of increase also observed (Reid et al, 1994), fenton reagent and crude oil (Onwurah, 1999). It has been reported that high lipid peroxidation products observed in liver and brain homogenates may be as a result of damage to the cell membrane caused by free radical attack (Onwurah, 1999). Similarly, Achuba (2005), also reported increase in the peroxidation product in his work on petroleum contaminated diet in rabbit.…”

Evaluation of haematological, hepatic and renal functions of petroleum tanker drivers in Lagos, Nigeria

“…The tanker drivers investigated in this study showed a significant increase (p ≤ 0.05) in their lipid peroxidation level compared with the control subjects. It may be the petrochemical activated metabolites that react with some cellular components such as membrane lipids to produce lipid peroxidation products 22 which may lead to membrane change and tissue damage. The increase in lipid peroxidation may also be responsible for the renal and hepatic damage observed in the petroleum tanker drivers as seen in this study.…”

Sub-chronic effect of Rambo insect powder contaminated diet on superoxide dismutase (SOD) activity in Wister albino rats

“…Autooxidation of epinephrine was initiated by adding 1ml of Fenton reagent prepared as described by Onwurah 13 . To a mixture of epinephrine (3 x10 -4 M), Na 2 CO 3 (10 -3 M), EDTA (10 -4 M), and 1.0ml of deionised water at a final volume of 6 ml.…”