Liquid platelet‐rich fibrin promotes the regenerative potential of human periodontal ligament cells

Abstract

Periodontitis is one of the principal causes for tooth loss characterized by the progressive degradation of alveolar bone and connective tissue attachment (Graves, Jiang, & Genco, 2000; Tonetti, Greenwell, & Kornman, 2018). At present, alveolar bone loss is considered irreversible, and various proposed therapies including guided tissue regeneration (GTR), subgingival scaling, and various regenerative approaches with growth factors/lasers remained suboptimal (Chen, Sun, Lu, & Yu, 2012; Pihlstrom, Michalowicz, & Johnson, 2005). Therefore, it is of great essence to figure out novel approaches. What is important for this task shall be attributed to the use of a regenerative and fully biocompatible scaffold aimed at inducing stem cell migration and differentiation and releasing growth factors to attenuate the inflammation environment so as to rebuild a favorable regenerative microenviron

Evaluation of the Addition of Injectable Platelet Rich Fibrin to Xenograft in Management of Periodontal Intraosseous Defects. “Randomized Controlled Trial”

“…This enhancement in clinical parameters might be attributed to the impact of i-PRF on soft tissue healing which was confirmed by Zheng et al when they investigated the impact of i-PRF on human periodontal ligament cells (PDLC). They found that i-PRF significantly enhanced human PDLC activity, differentiation and diminished notably the inflammation triggered by lipopolysaccharides (33) . Furthermore, Liu et al noticed an accelerated angiogenesis with an increased number of microvessels in the gingival tissue via upregulation of vascular endothelial growth factor after application of PRF (34) .…”

Ten years of injectable platelet‐rich fibrin

“…In summary, these findings indicate that the anti-inflammatory effect and regenerative potential of liquid PRF can counterbalance the negative inflammatory effect induced by LPSs. 20 These findings are specific to the periodontal field since PRF has been shown to improve the regeneration of intrabony and furcation defects, 21 and this is hypothesized to be caused not only by an improvement in local growth factor concentrations but also by counterbalancing the inflammatory response induced by LPSs.…”

“…The results of all assays were compared to those for PRP. 20 Osteogenic differentiation demonstrated that liquid PRF induced significantly greater ALP activity and more mineralized nodules when compared to PRP and controls (Figure 10). According to the experimental timeline, cells were then pretreated with or without LPSs for 7 days to induce an inflammatory condition, and then liquid PRF was added to the culture medium for an additional incubation period of 7 days (Figure 10A).…”

Clinical Application of Platelet-Rich Fibrin in Dermatology

“…3 Given its simple preparation, autologous nature, low cost, and biological properties. [4][5][6] In the field of dermatology, PRF has shown unique advantages in the treatment of various skin diseases and is expected to become a widely used and effective treatment. With the searching terms of "PRF," "platelet-rich fibrin," "PRP," "platelet-rich plasma" and "dermatology," we searched in PubMed for the literature from January, 2006 to January, 2021.…”

“…3 Given its simple preparation, autologous nature, low cost, and biological properties. 4-6 In the field of dermatology, PRF has shown unique advantages in the treatment of various skin diseases and is expected to become a widely used and effective treatment.…”