Localization of avian LHRH-immunoreactive neurons in the hypothalamus of the domestic fowl, Gallus domesticus, and the Japanese quail, Coturnix coturnix

Abstract

The localization of LHRH-containing perikarya and nerve fibers in the hypothalami of the domestic fowl and Japanese quail was investigated by means of the specific immunoperoxidase ABC method, using antisera against chicken LHRH-I ([Gln8]-LHRH), chicken GnRH-II ([His5-Trp7-Tyr8]-LHRH[2-10]) and mammalian LHRH ([Arg8]-LHRH). Chicken LHRH-I-immunoreactive perikarya were sparsely scattered in the nucleus preopticus periventricularis (POP), nucleus filiformis (FIL) and nucleus septalis medialis (SM), and in bilateral bands extending from these nuclei into the septal area in both species. A few reactive perikarya were also observed in the nucleus accumbens (Ac) and lobus parolfactorius (LPO). Numerous cLHRH-I-immunoreactive fibers were widely scattered in the preoptic, septal and tuberal areas, and were densely concentrated in the external layer of the median eminence and in organum vasculosum of the lamina terminalis (OVLT) in both species. Anti-mammalian LHRH serum cross-reacted weakly with perikarya and fibers immunoreactive to anti-cLHRH-I serum in normal chicken and quail. Anti-cGnRH-II[2-10] serum immunoreacted with magnocellular neurons distributed in the rostral end of the mesencephalon along the midline close to the nervus oculomotorius (N III). These perikarya were apparently different from cLHRH-I immunoreactive neurons. No immunoreactive cells and fibers against anti-cGnRH-II[2-10] were observed in the hypothalamus and median eminence of the chicken or quail. Anti-cGnRH-II[2-10] bound specifically with cGnRH-II.(ABSTRACT TRUNCATED AT 250 WORDS)

Changes in Feeding Activity, Plasma Luteinizing Hormone, and Testes Weight in Ring Doves Following Hypothalamic Injections of Prolactin

“…The finding that testes weights and LH levels in PRL-injected birds did not differ from those of vehicle-injected birds in either cannulation group suggests that other PRL-sensitive loci, such as the GnRH-rich tuberoinfundibular region (36)(37)(38)(39), may play a more important role than the POA or the VMN in mediating PRL-induced suppression of gonadotropin secretion. However, this result does not preclude a modest effect of the 25 ng PRL dose on gonadotropin secretion or gonadal weight following POA or VMN injection.…”

“…These sites were selected for analysis based on extensive evidence from lesion studies and neurochemical studies for VMN involvement in food intake regulation in birds (34, 35) and for gonadotropin-releasing hormone (GnRH)-containing cell bodies in the avian POA that are presumed to regulate pituitary gonadotropin secretion Brain sites of PRL action (36)(37)(38)(39).…”

Aspects of spermatogenesis and microscopic testicular morphology in Greater Rhea, Rhea americana (Linnaeus, 1758)

“…Some authors mention difficulties in the fixation of sections of the Quail, the Rooster and the Helmeted Guineafowl males (Nagano 1962, Lake 1971, Budras & Sauer 1975, Hess et al 1976, Gunawardana & Scott 1977, Hess & Thurston 1977, Aire 1979, 1982a, 1982b, Bakst 1980, Mikami et al 1988.…”

A second gene for gonadotropin-releasing hormone: cDNAand expression pattern in the brain.

“…1 (23)(24)(25)(26) and is the most effective form in stimulating ovulation when administered in vivo (7). This potency is surprising, since [His5,Trp7,Tyr8]GnRH is not the predominant GnRH isoform expressed in cells that project to the pituitary but, rather, is typically found in midbrain neuronal populations (27)(28)(29)(30)(31)(32)(33)(34). Moreover, neither expression levels (35) nor inhibition by specific antibodies of [His5,Trp7,Tyr8]GnRH correlates with the reproductive cycle (36).…”