Long noncoding RNA LINC01391 restrained gastric cancer aerobic glycolysis and tumorigenesis via targeting miR-12116/CMTM2 axis

Abstract

Increasing studies indicate that long noncoding RNA (lncRNA) plays a critical role in aerobic glycolysis of various tumors. However, the contribution of lncRNA in gastric cancer (GC) cell glycolysis is still poorly understood. The objective of this research was to investigate the functional role and mechanism of lncRNA long intergenic non-protein coding RNA 1391 (LINC01391) in the aerobic glycolysis and tumorigenesis of GC. Here, we report that LINC01391 was low expressed in GC tissues and cell lines. LINC01391 overexpression hampered GC cell proliferation, migration, invasion and aerobic glycolysis, while LINC01391 knockdown demonstrated the opposite effects. LINC01391 overexpression delayed the tumor growth in vivo. Furthermore, LINC01391 interacted with miR-12116, and miR-12116 interacted with CMTM2 in GC cells. And miR-12116 and CMTM2 participated in the inhibitory effects of LINC01391 on cell migration, invasion and aerobic glycolysis in GC cells. LINC01391 restrained aerobic glycolysis and tumorigenesis of GC via targeting miR-12116/CMTM2 axis, which provides new insights into mechanism of GC progression.

N6-methyladenosine Reader YTHDF1-induced lncRNA DLGAP1-AS2 Promotes the c-Myc Mediated Gastric Cancer Aerobic Glycolysis

“…For instance, the overexpressed LINC00242 promotes the GC proliferation and aerobic glycolysis in vitro and relieves the tumorigenesis in vivo through directly targeting G6PD 19 . LINC01391 overexpression hampers the proliferation, invasion and aerobic glycolysis in vitro and tumor growth in vivo of GC, while LINC01391 knockdown shows the opposite results 20 . Overexpressed DLX6-AS1 in GC signi cantly promotes cell viability and colony formation, and then regulates aerobic glycolysis by targeting phosphoinositidedependent protein kinase 1 (PDK1) 21 .…”

Long noncoding RNA DLGAP1-AS1 promotes the progression of glioma by regulating the miR-1297/EZH2 axis

“…Several studies have reported the close association of aberrant lncRNAs and miRNAs expressions to tumor development, including glioma [ 24 , 25 ]. lncRNA HAS2-AS1 promoted tumour progression in glioma by acting as a ceRNA [ 26 ].…”

“…HCG11 modulated glioma progression through its action on the miR-496/CPEB3 axis [ 27 ]. Moreover, DLGAP1-AS1 has also been implicated in several processes involved in tumor progression [ 24 , 25 ]. However, the expression and functional role of DLGAP1-AS1 in glioma has yet to be completely characterized.…”

Curcumin Antagonizes Glucose Fluctuation-Induced Renal Injury by Inhibiting Aerobic Glycolysis via the miR-489/LDHA Pathway

“…The mechanism of the Warburg effect is still unclear. We built a panel of 17 miRNAs [ 26 – 29 , 31 – 43 ] and 8 lncRNAs [ 29 , 30 , 32 , 35 , 36 , 43 – 45 ] that were previously reported to be regulators of the Warburg effect. The results indicated that GF reduced the miR-489 content in the HEK-293 cell model and a moderate dose of Cur (40 μ M) could prevent this downregulation.…”

“…In this study, we first measured the levels of aerobic glycolysis and evaluated Cur's protective effects in HEK-293 cells under the condition of oscillating high glucose. After that, we built a panel of 17 miRNAs [ 26 – 29 , 31 – 43 ] and 8 lncRNAs [ 29 , 30 , 32 , 35 , 36 , 43 – 45 ] that were previously reported to be regulators of the Warburg effect. After a range of experiments, we found miR-489/LDHA as vital modulator axis in kidney protection of Cur administration.…”