Long-term high fructose and saturated fat diet affects plasma fatty acid profile in rats

Tranchida, Fabrice; Tchiakpe, Léopold; Rakotoniaina, Zo; Deyris, Valérie; Ravion, Olivier; Hiol, Abel

doi:10.1631/jzus.b1100090

Cited by 30 publications

(21 citation statements)

References 61 publications

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“…After 10 weeks of diet the body weight of HFS, HFS+C and control rats was similar in agreement with previous researches [ 22 , 23 ] while a significant increase of relative liver weight was observed in both HFS groups. Compared to control, high fructose and saturated fatty acids diets were associated with a significant increase of glucose, triglycerides, cholesterol and insulin and HOMA-IR levels ( Table 2 ).…”

Section: Resultssupporting

confidence: 92%

“…In all experimental groups, the major plasma FA were: palmitic (16:0), palmitoleic (16:1n-7), stearic (18:0), oleic (18:1n-9), cis-vaccenic (18:1n-7), linoleic acid (18:2n-6), arachidonic acid (20:4n-6) and docosahexaenoic acid (22:6n-3 or DHA) ( Table 4 ). The change in FA composition between the controls and the HFS group was fully described in a previous study [ 22 ]. Basically, it was observed in the HFS group an increased activity of the delta-9 (Δ9D) and delta-6-desaturase (Δ6D), and a decrease of the delta-5-desaturase (Δ5D), as seen by the 20:4n-6/20:3n-6 ratio, in addition to the depletion of n-6 polyunsaturated fatty acids (n-6 PUFA), 18:2n-6 and its elongation and desaturation product, 20:4n-6.…”

Section: Resultsmentioning

confidence: 99%

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Metabolomic and Lipidomic Analysis of Serum Samples following Curcuma longa Extract Supplementation in High-Fructose and Saturated Fat Fed Rats

et al. 2015

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We explored, using nuclear magnetic resonance (NMR) metabolomics and fatty acids profiling, the effects of a common nutritional complement, Curcuma longa, at a nutritionally relevant dose with human use, administered in conjunction with an unbalanced diet. Indeed, traditional food supplements have been long used to counter metabolic impairments induced by unbalanced diets. Here, rats were fed either a standard diet, a high level of fructose and saturated fatty acid (HFS) diet, a diet common to western countries and that certainly contributes to the epidemic of insulin resistance (IR) syndrome, or a HFS diet with a Curcuma longa extract (1% of curcuminoids in the extract) for ten weeks. Orthogonal projections to latent structures discriminant analysis (OPLS-DA) on the serum NMR profiles and fatty acid composition (determined by GC/MS) showed a clear discrimination between HFS groups and controls. This discrimination involved metabolites such as glucose, amino acids, pyruvate, creatine, phosphocholine/glycerophosphocholine, ketone bodies and glycoproteins as well as an increase of monounsaturated fatty acids (MUFAs) and a decrease of n-6 and n-3 polyunsaturated fatty acids (PUFAs). Although the administration of Curcuma longa did not prevent the observed increase of glucose, triglycerides, cholesterol and insulin levels, discriminating metabolites were observed between groups fed HFS alone or with addition of a Curcuma longa extract, namely some MUFA and n-3 PUFA, glycoproteins, glutamine, and methanol, suggesting that curcuminoids may act respectively on the fatty acid metabolism, the hexosamine biosynthesis pathway and alcohol oxidation. Curcuma longa extract supplementation appears to be beneficial in these metabolic pathways in rats. This metabolomic approach highlights important serum metabolites that could help in understanding further the metabolic mechanisms leading to IR.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Metabolomic and Lipidomic Analysis of Serum Samples following Curcuma longa Extract Supplementation in High-Fructose and Saturated Fat Fed Rats

et al. 2015

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“…In general, the change in liver FA composition between the controls and the HFS group was similar to that previously described 7, 21 for serum. In short, a significant decrease of polyunsaturated fatty acids (PUFA, −27%) was observed in the HFS group while monounsatured fatty acids (MUFA) were increased (2 fold).…”

Section: Resultssupporting

confidence: 87%

Hepatic metabolic effects of Curcuma longa extract supplement in high-fructose and saturated fat fed rats

Tranchida

Rakotoniaina

Shintu

et al. 2017

Sci Rep

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The metabolic effects of an oral supplementation with a Curcuma longa extract, at a dose nutritionally relevant with common human use, on hepatic metabolism in rats fed a high fructose and saturated fatty acid (HFS) diet was evaluated. High-resolution magic-angle spinning NMR and GC/MS in combination with multivariate analysis have been employed to characterize the NMR metabolite profiles and fatty acid composition of liver tissue respectively. The results showed a clear discrimination between HFS groups and controls involving metabolites such as glucose, glycogen, amino acids, acetate, choline, lysophosphatidylcholine, phosphatidylethanolamine, and β-hydroxybutyrate as well as an increase of MUFAs and a decrease of n-6 and n-3 PUFAs. Although the administration of CL did not counteract deleterious effects of the HFS diet, some metabolites, namely some n-6 PUFA and n-3 PUFA, and betaine were found to increase significantly in liver samples from rats having received extract of curcuma compared to those fed the HFS diet alone. This result suggests that curcuminoids may affect the transmethylation pathway and/or osmotic regulation. CL extract supplementation in rats appears to increase some of the natural defences preventing the development of fatty liver by acting on the choline metabolism to increase fat export from the liver.

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“…Saturated fats are known to be hypercholesterolemic in men and laboratory animals but display lower oxidability of LDL . The Zucker Fa/Fa rat has been widely used as an animal model for obesity and cardiovascular disease studies .…”

Section: Introductionmentioning

confidence: 99%

Lipoproteinemia and arylesterase activity in Zucker Fa/Fa rats fed glucomannan/spirulina‐enriched squid‐surimi

Vázquez‐Velasco¹,

González‐Torres²,

Olivero‐David³

et al. 2013

Euro J Lipid Sci & Tech

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Surimi is considered a healthy food. Hypocholesterolemic and antioxidant properties of soluble fiber and microalgae may reduce cardiovascular disease risk, making them potential functional ingredients. This study aims to determine whether glucomannan-enriched or glucomannan plus spirulina-enriched squidsurimi improve plasma lipid/lipoprotein levels and arylesterase (AE) activity in Zucker Fa/Fa rats. Six groups of eight growing rats were given modified AIN-93M diets for 7 wk. Experimental diets included 30% squid-surimi control diet (C), 30% glucomannan-enriched squid-surimi diet (G), 30% glucomannan plus spirulina-enriched squid-surimi diet (GS), and the same diets enriched with hypercholesterolemic agent (2% cholesterol and 0.4% cholic acid; HC, HG, and HGS diets). G and GS diets induced significantly lower (p < 0.001) body weight gain and growth rate than the C diet and partially normalized the lipoprotein lipid contribution to plasma lipids. HC rats presented marked hyperlipemia, elevated cholesterol/triglyceride ratio in all lipoprotein fractions, and a high percentage of cholesterol transported by VLDL and IDL þ LDL fractions. HG and HGS diets noticeably normalized lipemia and the lipid composition of the various lipoprotein fractions versus HC. In conclusion, glucomannan added to surimi-diets decreased bodyweight gain. HG or HGS exhibited strong lipid normalizing properties versus HC. Spirulina did not add to the effects of glucomannan.

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Long-term high fructose and saturated fat diet affects plasma fatty acid profile in rats

Cited by 30 publications

References 61 publications

Metabolomic and Lipidomic Analysis of Serum Samples following Curcuma longa Extract Supplementation in High-Fructose and Saturated Fat Fed Rats

Metabolomic and Lipidomic Analysis of Serum Samples following Curcuma longa Extract Supplementation in High-Fructose and Saturated Fat Fed Rats

Hepatic metabolic effects of Curcuma longa extract supplement in high-fructose and saturated fat fed rats

Lipoproteinemia and arylesterase activity in Zucker Fa/Fa rats fed glucomannan/spirulina‐enriched squid‐surimi

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