Long-Term Recovery of the Fecal Microbiome and Metabolome of Dogs with Steroid-Responsive Enteropathy

Pilla, Rachel; Guard, Blake C.; Blake, Amanda; Ackermann, Mark R.; Webb, Craig B.; Hill, Steve; Lidbury, Jonathan A.; Steiner, Jörg M.; Jergens, Albert E.; Suchodolski, Jan S.

doi:10.3390/ani11092498

Cited by 16 publications

(16 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In dogs, preliminary studies would suggest that the microbiota may normalize after dietary (eg, normalization of C hiranonis ) 39 or immunosuppressive therapy. 40 Whether the feline DI could act as a monitoring biomarker, a prognostic biomarker or a predictive biomarker requires further investigations in prospective clinical studies.…”

Section: Discussionmentioning

confidence: 99%

Dysbiosis index to evaluate the fecal microbiota in healthy cats and cats with chronic enteropathies

Sung

Marsilio

Chow

et al. 2022

Journal of Feline Medicine and Surgery

Self Cite

View full text Add to dashboard Cite

Objectives Previous studies have identified various bacterial taxa that are altered in cats with chronic enteropathies (CE) vs healthy cats. Therefore, the aim of this study was to develop a targeted quantitative molecular method to evaluate the fecal microbiota of cats. Methods Fecal samples from 80 client-owned healthy cats and 68 cats with CE were retrospectively evaluated. A panel of quantitative PCR (qPCR) assays was used to measure the fecal abundance of total bacteria and seven bacterial taxa: Bacteroides, Bifidobacterium, Clostridium hiranonis, Escherichia coli, Faecalibacterium, Streptococcus and Turicibacter. The nearest centroid classifier algorithm was used to calculate a dysbiosis index (DI) based on these qPCR abundances. Results The abundances of total bacteria, Bacteroides, Bifidobacterium, C hiranonis, Faecalibacterium and Turicibacter were significantly decreased, while those of E coli and Streptococcus were significantly increased in cats with CE ( P <0.027 for all). The DI in cats with CE was significantly higher compared with healthy cats ( P <0.001). When the cut-off value of the DI was set at 0, it provided 77% (95% confidence interval [CI] 66–85) sensitivity and 96% (95% CI 89–99) specificity to differentiate the microbiota of cats with CE from those of healthy cats. Fifty-two of 68 cats with CE had a DI >0. Conclusions and relevance A qPCR-based DI for assessing the fecal microbiota of cats was established. The results showed that a large proportion of cats with CE had an altered fecal microbiota as evidenced by an increased DI. Prospective studies are warranted to evaluate the utility of this assay for clinical assessment of feline CE.

show abstract

Section: Discussionmentioning

confidence: 99%

Dysbiosis index to evaluate the fecal microbiota in healthy cats and cats with chronic enteropathies

Sung

Marsilio

Chow

et al. 2022

Journal of Feline Medicine and Surgery

Self Cite

View full text Add to dashboard Cite

show abstract

“…30 Enterobacteriaceae are Gram-facultative aerobic bacteria that can exacerbate inflammation. 31 In contrast, Fusobacteria are more representative of healthy microbiome in dogs and their decrement is associated with chronic enteropathies. 31 The inconsistency of this result with a clear clinical improvement still needs clarification and further studies.…”

Section: Discussionmentioning

confidence: 99%

“…In our study, there was also a significant increase in Enterococcaceae and Enterobacteriaceae , while Fusobacteriaceae were more abundant in the group of healthy dogs 30 . Enterobacteriaceae are Gram‐facultative aerobic bacteria that can exacerbate inflammation 31 …”

Section: Discussionmentioning

confidence: 99%

Clinical evaluation and microbiota analysis in 9 dogs with antibiotic‐responsive enteropathy: A prospective comparison study

Bottero

Ferriani

Benvenuti

et al. 2022

Veterinary Internal Medicne

View full text Add to dashboard Cite

Background: Antibiotic-responsive enteropathy (ARE) is diagnosed by excluding other causes of diarrhea and when there is a short-term response to administration of antibiotics.Objectives: To characterize the gut microbiota and clinical trend of dogs with suspected ARE and to evaluate the variation in microbiota before (T0), after 30 days (T30) of tylosin treatment, and 30 days after discontinuation of treatment (T60). A further objective was to evaluate whether changes in gut microbiota are related to relapses of diarrhea when the therapy is tapered.Animals: Study sample (group A) was composed of 15 dogs with chronic diarrhea, group B was composed of 15 healthy dogs. Group A was given tylosin for 30 days.Methods: A multicentric prospective study. Clinical Indexes, fecal score, and samples for microbiota analysis were collected at T0, T30, and T60 in group A and T0 and T30 in group B. The gut microbiota was analyzed via 16S ribosomal RNA gene. Qiime2 version 2020.2 was used to perform bioinformatic analyses, and Alpha-and Beta-diversity were computed.Results: Diarrhea recurred after T30 in 9 of 14 dogs, which were classified as affected by ARE. At T0, a difference was noted in the beta-diversity between groups (Bray Curtis metric P = .006). A T0-T30 difference in alpha-diversity was noted in group A (Shannon index P = .001, Faith PD P = .007).Conclusions and Clinical Importance: Although tylosin influences the microbiota of dogs with ARE, we failed to find any specific characteristic in the microbiota of dogs with ARE.

show abstract

“…One study in mice showed that cobalamin supplementation was associated with significantly altered beta diversity but not alpha diversity [ 20 ], whereas beta diversity was unaffected in three other studies in mice [ 17 , 21 , 22 ]. At the genus level, an abundance of Bacteroides has been reported to decrease with oral supplementation with cyanocobalamin [ 20 ], a concerning finding since Bacteroides is commonly already decreased in dogs with CE and expected to improve with treatment [ 23 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

The Intestinal Microbiome in Dogs with Chronic Enteropathies and Cobalamin Deficiency or Normocobalaminemia—A Comparative Study

Toresson

Suchodolski

Spillmann

et al. 2023

Animals

View full text Add to dashboard Cite

Cobalamin deficiency is a common sequela of chronic enteropathies (CE) in dogs. Studies comparing the intestinal microbiome of CE dogs with cobalamin deficiency to those that are normocobalaminemic are lacking. Therefore, our aim was to describe the fecal microbiome in a prospective, comparative study evaluating 29 dogs with CE and cobalamin deficiency, 18 dogs with CE and normocobalaminemia, and 10 healthy control dogs. Dogs with cobalamin deficiency were also analyzed after oral or parenteral cobalamin supplementation. Overall microbiome composition (beta diversity) at baseline was significantly different in CE dogs with cobalamin deficiency when compared to those with normocobalaminemia (p = 0.001, R = 0.257) and to healthy controls (p = 0.001, R = 0.363). Abundances of Firmicutes and Actinobacteria were significantly increased (q = 0.010 and 0.049), while those of Bacteroidetes and Fusobacteria were significantly decreased (q = 0.002 and 0.014) in CE dogs with cobalamin deficiency when compared to healthy controls. Overall microbiome composition in follow-up samples remained significantly different after 3 months in both dogs receiving parenteral (R = 0.420, p = 0.013) or oral cobalamin supplementation (R = 0.251, p = 0.007). Because cobalamin supplementation, in combination with appropriate therapy, failed to restore the microbiome composition in the dogs in our study, cobalamin is unlikely to be the cause of those microbiome changes but rather an indicator of differences in underlying pathophysiology that do not influence clinical severity but result in a significant aggravation of dysbiosis.

show abstract

Long-Term Recovery of the Fecal Microbiome and Metabolome of Dogs with Steroid-Responsive Enteropathy

Cited by 16 publications

References 58 publications

Dysbiosis index to evaluate the fecal microbiota in healthy cats and cats with chronic enteropathies

Dysbiosis index to evaluate the fecal microbiota in healthy cats and cats with chronic enteropathies

Clinical evaluation and microbiota analysis in 9 dogs with antibiotic‐responsive enteropathy: A prospective comparison study

The Intestinal Microbiome in Dogs with Chronic Enteropathies and Cobalamin Deficiency or Normocobalaminemia—A Comparative Study

Contact Info

Product

Resources

About