<i>In-vitro </i>Prebiotic Activity of Grape Seed Flour Highly Rich in Flavonoid and Dietary Fiber

Abstract

Interactions between lactic acid bacteria (LAB) and dietary polyphenols are not fully understood. This study determined the prebiotic effects of grape seed flour (GSF, a byproduct of wine making) and their polyphenol extracts (GSE) on the growth of 10 strains of probiotic LAB recently isolated from the kefir and pathogenic Clostridium perfringens. Growth rate of the LAB, Leuconostoc mesenteroides DH 1608, was the highest among 10 strains of LAB when cultivated with GSF. GSE, containing an equivalent amount of polyphenol in GSF, also promoted the growth of L. mesenteroides DH 1608 but at a lower rate compared to GSF. Growth of C. perfringens decreased significantly at 10 mg/mL of GSF, but GSE did not affect the growth. In conclusion, GSF may be a useful prebiotic dietary supplement due to its selective antimicrobial capacity via stimulation of probiotic bacteria and inhibition of pathogenic bacteria. A symbiotic combination of selected lactic acid bacteria and GSF may improve gut health.

Synbiotic Effect of Whole Grape Seed Flour and Newly Isolated Kefir Lactic Acid Bacteria on Intestinal Microbiota of Diet-Induced Obese Mice

“…Our study and other previous studies have shown that WGF or grape seed extract stimulates the growth of Lactobacillus spp. and inhibits Clostridium histolyticum. − Body and adipose tissue weights were closely related to modulation of the intestinal microbiota in diet-induced obese (DIO) mice fed diets containing WGF . These results suggest that the prebiotic action of WGF can improve obesity and obesity-related diseases.…”

“…Furthermore, we isolated Lactobacillus kefiri DH5 that, when fed to mice, reduced obesity and fatty liver disease by upregulating genes for fatty acid oxidation in adipose tissue, supporting a probiotic role and specific mechanism for this bacterium. Recently, we have shown that WGF stimulates the in vitro growth of L. kefiri DH5, 14 suggesting that the combination of WGF and LAB has potential synbiotic benefits as pre-and probiotics, respectively. Indeed, we reported that the synergistic amelioration of highfat (HF)-induced obesity and metabolic dysfunctions in a study focused on the relationship between physiological biomarkers and gene expression of liver and adipose tissues using a microarray analysis.…”

“…Recently, we have shown that WGF stimulates the in vitro growth of L. kefiri DH5, suggesting that the combination of WGF and LAB has potential synbiotic benefits as pre- and probiotics, respectively. Indeed, we reported that the synergistic amelioration of high-fat (HF)-induced obesity and metabolic dysfunctions in a study focused on the relationship between physiological biomarkers and gene expression of liver and adipose tissues using a microarray analysis. , Others showed synbiotic effects of prebiotics using a fermentable oligosaccharide or polysaccharide. − …”

Potential of Persimmon Dietary Fiber Obtained from Byproducts as Antioxidant, Prebiotic and Modulating Agent of the Intestinal Epithelial Barrier Function

“…To test the biological potential of extracted fiber and the effects of the digestion process, fermentation was performed before and after the in vitro gastrointestinal digestion on selected beneficial host microorganisms according to the methodologies established by [ 27 , 28 , 29 , 30 ]. A 10 mL volume of each homogenized fraction and control were centrifuged at 948× g , 10 min at 4 °C, and the supernatants and pellets were separated.…”