Luteolin-rich chrysanthemum flower extract suppresses baseline serum uric acid in Japanese subjects with mild hyperuricemia

Hirano, Marina; Takeda, Shogo; Hitoe, Shoketsu; Shimoda, Hiroshi

doi:10.15761/imm.1000275

Cited by 9 publications

(10 citation statements)

References 19 publications

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“…The single oral dosing study showed no significant changes in serum uric acid levels between the CFE and placebo groups (Figure 2). This result was consistent with our previous findings [15]. Serum uric acid after 12-week ingestion of CFE, which was set as the primary outcome, was significantly lower in the CFE group than in the placebo group (Table 2).…”

Section: Discussionsupporting

confidence: 92%

“…Ltd. [15]. Collectively, these findings indicate that the consecutive ingestion of CFE decreases serum uric acid levels.…”

Section: Discussionmentioning

confidence: 70%

“…Luteolin has been shown to inhibit the activity of xanthine oxidase, which is a uric acid synthase [14]. In our previous clinical trial, we demonstrated that the consumption of luteolin-rich chrysanthemum flower extract (CFE) for 4 weeks decreased serum uric acid levels in Japanese men [15]. Since CFE did not suppress purine base absorption from the intestines [15], its effects appeared to depend on xanthine oxidase.…”

Section: Introductionmentioning

confidence: 89%

“…In our previous clinical trial, we demonstrated that the consumption of luteolin-rich chrysanthemum flower extract (CFE) for 4 weeks decreased serum uric acid levels in Japanese men [15]. Since CFE did not suppress purine base absorption from the intestines [15], its effects appeared to depend on xanthine oxidase. However, we only enrolled male subjects; therefore, the effects of CFE on females remain unknown.…”

Section: Introductionmentioning

confidence: 97%

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Effects of luteolin-rich chrysanthemum flower extract on purine base absorption and blood uric acid in Japanese subjects

Takara

Yamamoto²,

Suzuki³

et al. 2022

FFHD

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Background and objective: Chrysanthemum flowers are consumed as fresh condiments, herbal teas, and processed foods in Japan and Taiwan. They contain luteolin as a major polyphenol and are traditionally used for eye care. We previously demonstrated that the ingestion of Chrysanthemum flower extract (CFE) for 1 month reduced serum uric acid levels. However, the findings obtained were considered to be biased because the study was performed by a CFE manufacturer. Therefore, we herein conducted a clinical trial on CFE on a larger scale and examined its effects on purine base absorption from the intestines, which represents an effective approach for reducing serum uric acid levels. Methods: Both studies were performed as randomized, double-blind, placebo-controlled trials and CFE (100 mg) containing 1 mg of luteolin was used as the active sample. We enrolled 44 healthy Japanese men and women with 6.0 to 7.9 mg/dL serum uric acid. All subjects were randomly allocated to an active group (n=22) or placebo group (n=22) using a computerized random number generator. In the purine base absorption study, CFE was ingested with a purine base-rich diet and serum uric acid levels were measured chronologically. In the 12-week consecutive ingestion study, CFE or placebo was administered between January and April 2021. Serum uric acid levels after 12 weeks were assessed as the primary outcome, and uric acid were measured before and after 4 weeks of the intervention as secondary outcomes. Blood, urine and body parameters were examined to evaluate the safety of CFE. Results: Thirty-nine subjects completed the trial, and the per protocol set comprised 18 and 21 subjects in the active and placebo groups, respectively. In the single dosing study of CFE on subjects loaded by the purine base-rich diet, no significant changes were observed between the CFE and placebo groups. On the other hand, in the 12-week ingestion study, serum uric acid levels were significantly lower in the CFE group than in the placebo group. Laboratory tests revealed no abnormalities to suggest any side effects of CFE.Conclusions: CFE (100 mg/day) containing 1 mg of luteolin reduced serum uric acid levels. CFE may be beneficial for improving hyperurichemia. Trial Registration: UMIN-CTR: UMIN000042327Foundation: The present study was funded by Oryza Oil & Fat Chemical Co., Ltd. Keywords: Chrysanthemum, luteolin, uric acid, purine base

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Section: Discussionsupporting

confidence: 92%

“…Ltd. [15]. Collectively, these findings indicate that the consecutive ingestion of CFE decreases serum uric acid levels.…”

Section: Discussionmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 97%

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Effects of luteolin-rich chrysanthemum flower extract on purine base absorption and blood uric acid in Japanese subjects

Takara

Yamamoto²,

Suzuki³

et al. 2022

FFHD

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“…Many in vitro studies have been performed to investigate the XO inhibitory mechanism of phenolic acids and bioactive flavonoids, such as chrysin, kaempferol and quercetin [5,6,7]. Numerous in vivo studies on the anti-hyperuricemic activity of other plant extracts have also been conducted [5,8], but investigation on C. molifolium has not been well performed in a greater depth. Therefore, the present study is the first in vitro and in vivo investigation on the potential of C. morifolium flower sub-fractions in XO inhibition and the determination of its antihyperuricemic activity followed by XO mRNA gene expression study.…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo inhibition of xanthine oxidase by the flowers of Chrysanthemum morifolium Ramat.

Ng¹,

Loh²,

Tan³

et al. 2020

Preprint

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Background: Xanthine oxidase (XO) plays an important role in human’s purine degradation. Excessive uric acid formation results in hyperuricemia and gout. The study aimed to determine the XO inhibitory potential of Chrysanthemum morifolium Ramat. dried flower and its effect on XO gene expression in animal models.Methods: In vitro XO inhibitory assay was employed to investigate the XO inhibitory potential of C. morifolium flowers. The bioactive sub-fraction was investigated further to give additional insight on its uric acid lowering potential via animal study and XO gene expression analysis. HPLC-Q-TOF-MS/MS was utilized to identify the putative compounds in the sub-fraction.Results: Among the fractions, EtOAc fraction exhibited the highest in vitro XO inhibitory potency (51.77 ± 0.98%; IC50 = 10.64 ± 0.51 µg/mL) and it was further fractionated into 15 sub-fractions through open column chromatography. EtOAc F7, F8, F9, F10, and F11 possessed >75% XO inhibition. F9 and F10 exhibited high in vitro XO inhibitory activity, cellular pro-proliferative effect and intracellular antioxidant activity among the sub-fractions tested. These two sub-fractions were also non-cytotoxic at the concentration range of 0.1 – 10 µg/mL. F10 was shown to be very effective in both serum and urine uric acid lowering properties in rats model upon oral consumption. It was subjected to further fractionation and a total of 11 sub-fractions were obtained. F10-4, F10-8, F10-9, and F10-10 possessed >90% XO inhibition. These sub-fractions were subjected to HPLC-Q-TOF-MS/MS analysis. A total of nine known compounds have been identified and 26 unknown compounds were detected. Conclusions: The possible mechanisms contributed to the anti-hyperuricemic effect of F10 were suggested to be non-competitive inhibition of XO enzyme, XO gene expression down regulation, and enhancement of uric acid excretion. Structure elucidation of the unknown compounds and the evaluation of the XO inhibitory activity of a single or a mixture of these compounds are recommended to identify possible synergism between them.

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Application of multivariate linear regression models for selection of deep eutectic solvent for extraction of apigenin and luteolin from Chrysanthemum indicum L.

Thu

Huyen

Phuong

2021

Phytochemical Analysis

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Introduction Among a variety of compounds presented in chrysanthemum, apigenin and luteolin are the two main components that play a major role in numerous biological activities of this herb. Objectives We aimed to obtain linear models showing the dependence of the yield of extraction of apigenin and luteolin on the composition of deep eutectic solvent and investigate the extraction of these two ingredients from Chrysanthemum indicum L. Methods Two models showing the dependence of luteolin and apigenin concentrations on the composition of the solvent were established using a multilinear regression algorithm and were applied to screen 119 different solvents. After that, the extraction process was optimized using response surface methodology and an artificial neural network. Apigenin and luteolin were recovered from the extract by the combination of distillation and addition of water. Results The screening results on 119 solvents revealed that choline chloride–acetic acid (1:4) was the most suitable deep eutectic solvent. It was showed that both response surface methodology and the artificial neural network could accurately determine the optimal conditions of extraction of apigenin and luteolin from C. indicum L., including time of extraction (65 minutes), temperature of extraction (90°C) and water content (20%). By the combination of distillation and addition of water, apigenin and luteolin could be effectively recovered from the deep eutectic solvent extract with a recovery rate of over 80%. Conclusions Deep eutectic solvent could be used as an effective green alternative to the conventional solvents for the extraction of bioactive compounds from plants.

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Luteolin-rich chrysanthemum flower extract suppresses baseline serum uric acid in Japanese subjects with mild hyperuricemia

Cited by 9 publications

References 19 publications

Effects of luteolin-rich chrysanthemum flower extract on purine base absorption and blood uric acid in Japanese subjects

Effects of luteolin-rich chrysanthemum flower extract on purine base absorption and blood uric acid in Japanese subjects

In vitro and in vivo inhibition of xanthine oxidase by the flowers of Chrysanthemum morifolium Ramat.

Application of multivariate linear regression models for selection of deep eutectic solvent for extraction of apigenin and luteolin from Chrysanthemum indicum L.

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