Lysine decarboxylase transgenic tobacco root cultures biosynthesize novel hydroxycinnamoylcadaverines

Berlin, Jochen; Mollenschott, C.; Herminghaus, S.; Fecker, Lothar F.

doi:10.1016/s0031-9422(97)01098-4

Cited by 17 publications

(7 citation statements)

References 13 publications

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“…LDC activity could be regarded as one limiting factor in the synthesis of cadaverine‐derived secondary metabolites (Herminghaus et al 1991). Berlin et al (1998) reported that the biosynthesis of phenylpropanoid‐polyamine conjugates can be stimulated by overexpression of a heterologous bacterial LDC . Detection of the activity of HsLDC will facilitate the elucidation of the biosynthesis processes of lycopodium alkaloids in H. serrata .…”

Section: Resultsmentioning

confidence: 99%

Analysis of expressed sequence tags from theHuperzia serrataleaf for gene discovery in the areas of secondary metabolite biosynthesis and development regulation

Luo

Sun

Liu

et al. 2010

Physiologia Plantarum

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Huperzia serrata produces various types of lycopodium alkaloids, especially the huperzine A (HupA) that is a promising drug candidate for Alzheimer's disease. Despite the medicinal importance of H. serrata, little genomic or transcriptomic data are available from the public databases. A cDNA library was thus generated from RNA isolated from the leaves of H. serrata. A total of 4012 clones were randomly selected from the library, and 3451 high-quality expressed sequence tags (ESTs) were assembled to yield 1510 unique sequences with an average length of 712 bp. The majority (79.4%) of the unique sequences were assigned to the putative functions based on the BLAST searches against the public databases. The functions of these unique sequences covered a broad set of molecular functions, biological processes and biochemical pathways according to GO and KEGG assignments. The transcripts involved in the secondary metabolite biosynthesis of alkaloids, terpenoids and flavone/flavonoids, such as cytochrome P450, lysine decarboxylase (LDC), flavanone 3-hydroxylase, squalene synthetase and 2-oxoglutarate 3-dioxygenase, were well represented by 34 unique sequences in this EST dataset. The corresponding peptide sequence of the LDC contained the Pfam 03641 domain and was annotated as a putative LDC. The unique sequences encoding transcription factors, phytohormone biosynthetic enzymes and signaling components were also found in this EST collection. In addition, a total of 501 potential SSR-motif microsatellite loci were identified from the 393 H. serrata leaf unique sequences. This set of non-redundant ESTs and the molecular markers obtained in this study will establish valuable resources for a wide range of applications including gene discovery and identification, genetic mapping and analysis of genetic diversity, cultivar identification and marker-assisted selections in this important medicinal plant.

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Section: Resultsmentioning

confidence: 99%

Analysis of expressed sequence tags from theHuperzia serrataleaf for gene discovery in the areas of secondary metabolite biosynthesis and development regulation

Luo

Sun

Liu

et al. 2010

Physiologia Plantarum

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“…This offers new tools to study metabolic regulation and may create valuable opportunities to synthesise new products. Examples of the modification of plant metabolism by the expression of bacterial genes include the introduction of the capacity for polyhydroxybutyrate synthesis into Arabidopsis thaliana and Gossypium hirsutum (Poirier et al 1992;John and Keller 1996); the formation of uncharacteristic cadaverine-derived alkaloids and hydroxycinnamate conjugates in plants and hairy-root cultures of Nicotiana species expressing a bacterial lysine decarboxylase (Herminghaus et al 1996;Berlin et al 1998); enhancement of the production of the anthraquinone, alizarin, in hairy roots of Rubia peregrina by the introduction of isochorismate synthase (Lodhi et al 1996); and finally, the formation of 4-hydroxybenzoate glucoside conjugates in plants and cell cultures of Nicotiana tabacum as a result of expressing chorismate: pyruvate lyase (CPL) from Escherichia coli (Siebert et al 1996;Li et al 1997).…”

Section: Introductionmentioning

confidence: 99%

4-Hydroxycinnamoyl-CoA hydratase/lyase, an enzyme of phenylpropanoid cleavage from Pseudomonas , causes formation of C 6 -C 1 acid and alcohol glucose conjugates when expressed in hairy roots of Datura stramonium L.

Mitra

Mayer

Mellon

et al. 2002

Planta

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4-Hydroxycinnamoyl-CoA hydratase/lyase (HCHL), a crotonase homologue of phenylpropanoid catabolism from Pseudomonas fluorescens strain AN103, led to the formation of 4-hydroxybenzaldehyde metabolites when expressed in hairy root cultures of Datura stramonium L. established by transformation with Agrobacterium rhizogenes. The principal new compounds observed were the glucoside and glucose ester of 4-hydroxybenzoic acid, together with 4-hydroxybenzyl alcohol- O-beta- D-glucoside. In lines actively expressing HCHL, these together amounted to around 0.5% of tissue fresh mass. No protocatechuic derivatives were found, although a trace of vanillic acid-beta- D-glucoside was detected. There was no accumulation of 4-hydroxybenzaldehydes, whether free or in the form of their glucose conjugates. There was some evidence suggesting a diminished availability of feruloyl-CoA for the production of feruloyl putrescine and coniferyl alcohol. The findings are discussed in the context of a diversion of phenylpropanoid metabolism, and the ability of plants and plant cultures to conjugate phenolic compounds.

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“…The root culture of tobacco contained Cad is being metabolized in secondary metabolites like hydoxy cinnamoyl cadaverine. 69 Barley contained Cad and aminopropyl Cad APC as well as N, N bis 3-amino propyl Cad. 70 The micropropagated carnation plants also exhibit high level of Cad conjugates.…”

Section: In Cultured Tissuesmentioning

confidence: 99%

“…The transgenic Nicotiana glauca hairy root cultures containing the gene coding for a bacterial LDC showed enhanced levels of lysine decarboxylase, resulting into Cad and derived in to anabasine alkaloid and hydroxylcinnamoyl. 68,69 Cad is considered as precursor for piperidine alkaloids in Nicotiana and the quinolizidine alkaloids in Lupinus. 96 Cadaverine pyruvate transaminase is principal step in quinolizidine alkaloid biosynthesis in Lupinus cell culturing, 96 but not in the synthesis of tropane alkaloid in case of datura, whereas in tobacco, almost all alkaloid synthesis is induced after feeding Cad to cotyledons.…”

Section: Cadaverine Catabolismmentioning

confidence: 99%

Cadaverine

Tomar

Lakra

Mishra

2013

Plant Signaling & Behavior

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Lysine decarboxylase transgenic tobacco root cultures biosynthesize novel hydroxycinnamoylcadaverines

Cited by 17 publications

References 13 publications

Analysis of expressed sequence tags from theHuperzia serrataleaf for gene discovery in the areas of secondary metabolite biosynthesis and development regulation

Analysis of expressed sequence tags from theHuperzia serrataleaf for gene discovery in the areas of secondary metabolite biosynthesis and development regulation

4-Hydroxycinnamoyl-CoA hydratase/lyase, an enzyme of phenylpropanoid cleavage from Pseudomonas , causes formation of C 6 -C 1 acid and alcohol glucose conjugates when expressed in hairy roots of Datura stramonium L.

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