Magnetic Force-Driven Graphene Patterns to Direct Synaptogenesis of Human Neuronal Cells

Min, Kyung-Joon; Kim, Tae-Hyung; Choi, Jeong‐Woo

doi:10.3390/ma10101151

Cited by 12 publications

(12 citation statements)

References 58 publications

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“…In line with these results, recent findings have proven the ability of magnetic-force-driven GO hybrid patterns containing magnetic nanoparticles to control the accumulation and expression of synaptophysin in human neural cell cultures. 43 As some recent findings have revealed certain degree of biodegradability for GO, 44 we also focused on looking for ultrastructural cues of microfiber degradation. Interestingly, neither signs of microfiber structure disassembly nor degradation of the rGO sheets composing it was observed during the 21-day culture.…”

Section: Results and Discussionmentioning

confidence: 99%

Favorable Biological Responses of Neural Cells and Tissue Interacting with Graphene Oxide Microfibers

et al. 2017

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Neural tissue engineering approaches show increasing promise for the treatment of neural diseases including spinal cord injury, for which an efficient therapy is still missing. Encouraged by both positive findings on the interaction of carbon nanomaterials such as graphene with neural components and the necessity of more efficient guidance structures for neural repair, we herein study the potential of reduced graphene oxide (rGO) microfibers as substrates for neural growth in the injured central neural tissue. Compact, bendable, and conductive fibers are obtained. When coated with neural adhesive molecules (poly-l-lysine and N-cadherin), these microfibers behave as supportive substrates of highly interconnected cultures composed of neurons and glial cells for up to 21 days. Synaptic contacts close to rGO are identified. Interestingly, the colonization by meningeal fibroblasts is dramatically hindered by N-cadherin coating. Finally, in vivo studies reveal the feasible implantation of these rGO microfibers as a guidance platform in the injured rat spinal cord, without evident signs of subacute local toxicity. These positive findings boost further investigation at longer implantation times to prove the utility of these substrates as components of advanced therapies for enhancing repair in the damaged central neural tissue including the injured spinal cord.

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Section: Results and Discussionmentioning

confidence: 99%

Favorable Biological Responses of Neural Cells and Tissue Interacting with Graphene Oxide Microfibers

et al. 2017

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“…In primary mouse experiments, drug-entrapped IONPs demonstrated good biocompatibility and effective therapy for spinal cord injury [499]. Finally, Min et al developed a magnetic field-driven graphene oxide and IONP-based hybrid pattern that enabled stable and controlled neuronal cell growth and specific cell patterning [500]. Some strategies also rely on biocomposites that have been pre-loaded with cells.…”

Section: Spinal Cord Regeneration By Ionp-containing Biocompositesmentioning

confidence: 99%

Iron Oxide Nanoparticles in Regenerative Medicine and Tissue Engineering

2021

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In recent years, many promising nanotechnological approaches to biomedical research have been developed in order to increase implementation of regenerative medicine and tissue engineering in clinical practice. In the meantime, the use of nanomaterials for the regeneration of diseased or injured tissues is considered advantageous in most areas of medicine. In particular, for the treatment of cardiovascular, osteochondral and neurological defects, but also for the recovery of functions of other organs such as kidney, liver, pancreas, bladder, urethra and for wound healing, nanomaterials are increasingly being developed that serve as scaffolds, mimic the extracellular matrix and promote adhesion or differentiation of cells. This review focuses on the latest developments in regenerative medicine, in which iron oxide nanoparticles (IONPs) play a crucial role for tissue engineering and cell therapy. IONPs are not only enabling the use of non-invasive observation methods to monitor the therapy, but can also accelerate and enhance regeneration, either thanks to their inherent magnetic properties or by functionalization with bioactive or therapeutic compounds, such as drugs, enzymes and growth factors. In addition, the presence of magnetic fields can direct IONP-labeled cells specifically to the site of action or induce cell differentiation into a specific cell type through mechanotransduction.

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“…For example, aligned structures were quite vital in neural and musculoskeletal tissue since these cell types preferred an ordered orientation (Li, Mauck, Cooper, Yuan, & Tuan, 2007; Wang et al, 2009). Furthermore, the aligned surfaces pattern could control neural cell adhesion and guide axonal alignment by offering contact guidance, which was ultimately beneficial to guide synaptogenesis and synaptic junction formation of different neurons (Min, Kim, & Choi, 2017). The nerve stem cells on aligned nanofibrous scaffolds were evaluated to possess the superior capacity to direct nerve cell elongation and neurite outgrowth (Ghasemi‐Mobarakeh, Prabhakaran, Morshed, Nasr‐Esfahani, & Ramakrishna, 2008).…”

Section: Introductionmentioning

confidence: 99%

Aligned graphene/silk fibroin conductive fibrous scaffolds for guiding neurite outgrowth in rat spinal cord neurons

Liu

Wang

Yang

et al. 2020

J Biomedical Materials Res

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Graphene, as a highly conducting material, incorporated into silk fibroin (SF) substrates is promising to fabricate an electroactive flexible scaffold toward neural tissue engineering. It is well known that aligned morphology could promote cell adhesion and directional growth. The purpose of this study was to develop aligned conductive scaffolds made of graphene and SF (G/SF) by electrospinning technique for neural tissue engineering applications. The physicochemical characterization of scaffolds revealed that the mechanical and electrochemical property of aligned G/SF scaffolds continually raised with the increasing contents of graphene (A0% G/SF, A1% G/SF, A2% G/SF, and A3% G/SF), but the mechanical property descended when the graphene concentration reached to 4% (the A4% G/SF group). The results of the cell experiment in vitro indicated that all the aligned G/SF scaffolds were no neurotoxic to primary cultured spinal cord neurons. In addition, the neurite elongation in all aligned groups was significantly enhanced by the upregulation of Netrin‐1 expression compared to them in the control group. Thus, A3% G/SF scaffolds not only possessed the optimal property based on the mechanical and electrochemical performances but also displayed the beneficial capability to neurite outgrowth, which might perform a suitable candidate to successfully scaffold electrically active tissues during neural regeneration or engineering.

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Magnetic Force-Driven Graphene Patterns to Direct Synaptogenesis of Human Neuronal Cells

Cited by 12 publications

References 58 publications

Favorable Biological Responses of Neural Cells and Tissue Interacting with Graphene Oxide Microfibers

Favorable Biological Responses of Neural Cells and Tissue Interacting with Graphene Oxide Microfibers

Iron Oxide Nanoparticles in Regenerative Medicine and Tissue Engineering

Aligned graphene/silk fibroin conductive fibrous scaffolds for guiding neurite outgrowth in rat spinal cord neurons

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