Maintaining Integrity Under Stress: Envelope Stress Response Regulation of Pathogenesis in Gram-Negative Bacteria

Cho, Timothy; Rowley, Gary; Raivio, Tracy

doi:10.3389/fcimb.2019.00313

Cited by 118 publications

(135 citation statements)

References 273 publications

(419 reference statements)

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“…Additionally, the higher number of up-regulated genes involved in energy metabolism suggests stress responses [24]. Further, nine up-regulated genes involved in cell envelope synthesis indicate possible envelope stress responses [25].…”

Section: Discussionmentioning

confidence: 99%

The Anti-Campylobacter Activity and Mechanisms of Pinocembrin Action

et al. 2019

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We investigated the anti-Campylobacter activity of pinocembrin and its mechanism of action, as well as Campylobacter responses to pinocembrin treatment at the genetic and phenotypic levels, using C. jejuni NCTC 11168 and a multidrug efflux system repressor mutant (11168ΔcmeR). At its minimal inhibitory concentration, pinocembrin significantly increased cell membrane permeability of Campylobacter. Interestingly, at sub-inhibitory concentrations, pinocembrin did not significantly alter membrane functionality and it increased bacterial fitness. Treatment with pinocembrin evoked decreased expression of ribosomal proteins and down-regulation of several NADH dehydrogenase I chain subunits and proteins involved in iron uptake. This suggests altered protein production and redox cycle and iron metabolism. Interestingly, the chelation of Fe ions during the treatment with pinocembrin increased C. jejuni survival, although there was no increase in the formation of reactive oxygen species. Pre-treatment of C. jejuni with sub-inhibitory concentrations of pinocembrin for 2 h resulted in a 1 log decrease in C. jejuni colony forming units in mice liver at 8 days post-infection, compared to untreated C. jejuni. These findings suggest that pinocembrin modulates the metabolic activity of C. jejuni and that pre-treatment of C. jejuni with pinocembrin influences its virulence potential in mice. This anti-Campylobacter potential of pinocembrin warrants further investigation.

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Section: Discussionmentioning

confidence: 99%

The Anti-Campylobacter Activity and Mechanisms of Pinocembrin Action

et al. 2019

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“…We also observed the constitutive upregulation of loci RPPX_14680-14875 which encode homologs of sigma factor E (RpoE), anti-sigma factor RseAB, and DegP protein respectively. This cluster is known to orchestrate the expression of chaperone proteins as a stress response to the elevated amount of misfolded proteins in E.coli [30]. Additionally, these sigma factors are known to negatively regulate alginate biosynthesis in P. aeruginosa [31].…”

Section: Chaperonesmentioning

confidence: 99%

Adaptive laboratory evolution restores solvent tolerance in plasmid-curedPseudomonas putidaS12; a molecular analysis

Kusumawardhani

Furtwängler

Blommestijn

et al. 2020

Preprint

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Pseudomonas putida S12 is intrinsically solvent-tolerant and constitutes a promising platform for biobased production of aromatic compounds and biopolymers. The genome of P. putida S12 consists of a 5.8 Mbp chromosome, and a 580 kbp megaplasmid pTTS12 that carries several gene clusters involved in solvent tolerance. Removal of pTTS12 caused a significant reduction in solvent tolerance. In this study, we succeeded in restoring solvent tolerance in plasmid-cured P. putida S12 using adaptive laboratory evolution (ALE), underscoring the innate solvent-tolerance of this strain. Whole genome sequencing revealed several single nucleotide polymorphisms (SNPs) and a mobile element insertion, enabling ALE-derived strains to survive and sustain growth in the presence of a high toluene concentration (10% v/v). Mutations were identified in an RND efflux pump regulator arpR, resulting in constitutive upregulation of the multifunctional efflux pump ArpABC. SNPs were also found in the intergenic region and subunits of ATP synthase, RNA polymerase subunit B, global two-component regulatory system (GacA/GacS) and a putative AraC-family transcriptional regulator Afr. RNA-seq analysis further revealed a constitutive down-regulation of energy consuming activities in ALE-derived strains, including flagellar assembly, F0F1 ATP synthase, and membrane transport proteins. Out results indicate that constitutive expression of an alternative solvent extrusion pump in combination with high metabolic flexibility ensures restoration of solvent-tolerance in P. putida S12 lacking its megaplasmid.

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“…Crucial steps in the pathogen infection cycle include the capacity to limit and repair damages during host-related stress conditions, to escape the immunity system and to develop antibiotic resistance [ 17 , 18 , 19 ]. The regulation of all these steps during host colonization and infection is tightly regulated in pathogens, and sRNAs are important actors in these regulatory processes [ 16 ].…”

Section: Srnas Regulating Host-pathogen Interactionsmentioning

confidence: 99%

“…The envelope is a physical barrier that protects against environmental stresses and constitutes a crucial interface for host–pathogen interactions. To adapt to the host environment, bacteria have developed general stress responses and Envelope Stress Responses (ESR) [ 17 , 99 ]. The alternative sigma factors σ S or σ B orchestrate the general stress reponse in Gram-negative bacteria and Gram-positive bacteria respectively [ 99 ], while ERSs are mediated by the alternative sigma factor σ E and several TCSs including PhoPQ and EnvZ/OmpR.…”

Section: Srnas Regulating Host-pathogen Interactionsmentioning

confidence: 99%

Interplay between Regulatory RNAs and Signal Transduction Systems during Bacterial Infection

Piattelli

Peltier

Soutourina

2020

Genes

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The ability of pathogenic bacteria to stably infect the host depends on their capacity to respond and adapt to the host environment and on the efficiency of their defensive mechanisms. Bacterial envelope provides a physical barrier protecting against environmental threats. It also constitutes an important sensory interface where numerous sensing systems are located. Signal transduction systems include Two-Component Systems (TCSs) and alternative sigma factors. These systems are able to sense and respond to the ever-changing environment inside the host, altering the bacterial transcriptome to mitigate the impact of the stress. The regulatory networks associated with signal transduction systems comprise small regulatory RNAs (sRNAs) that can be directly involved in the expression of virulence factors. The aim of this review is to describe the importance of TCS- and alternative sigma factor-associated sRNAs in human pathogens during infection. The currently available genome-wide approaches for studies of TCS-regulated sRNAs will be discussed. The differences in the signal transduction mediated by TCSs between bacteria and higher eukaryotes and the specificity of regulatory RNAs for their targets make them appealing targets for discovery of new strategies to fight against multi-resistant bacteria.

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Maintaining Integrity Under Stress: Envelope Stress Response Regulation of Pathogenesis in Gram-Negative Bacteria

Cited by 118 publications

References 273 publications

The Anti-Campylobacter Activity and Mechanisms of Pinocembrin Action

The Anti-Campylobacter Activity and Mechanisms of Pinocembrin Action

Adaptive laboratory evolution restores solvent tolerance in plasmid-curedPseudomonas putidaS12; a molecular analysis

Interplay between Regulatory RNAs and Signal Transduction Systems during Bacterial Infection

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