Male age is associated with sperm DNA/chromatin integrity

Mettler, Anupama Deenadayal; Govindarajan, Mirudhubashini; Srinivas, Sapna; Mithraprabhu, Sridurga; Evenson, Donald P.; Mahendran, Tara

doi:10.1080/13685538.2019.1600496

Cited by 34 publications

(21 citation statements)

References 36 publications

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“…A positive correlation was detected between male age and the sperm DNA fragmentation level in all subjects. This result is consistent with those of previous studies demonstrating that sperm DNA fragmentation is linked to paternal age in men with normozoospermia (Belloc, Benkhalifa, Cohen‐Bacrie, Dalleac, Amar, et al, ; Das et al, ) and infertile males (Deenadayal Mettler et al, ). In contrast, Nijs et al () reported that sperm DNA fragmentation is not correlative with paternal age by SCSA, and Brahem, Mehdi, Elghezal, and Saad () concluded that male age has no effect on sperm DNA fragmentation by TUNEL assay in unclassified infertile men.…”

Section: Discussionsupporting

confidence: 93%

Male age is more critical to sperm DNA integrity than routine semen parameters in Chinese infertile males

et al. 2019

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Section: Discussionsupporting

confidence: 93%

Male age is more critical to sperm DNA integrity than routine semen parameters in Chinese infertile males

et al. 2019

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“…It should be noted that a recent report in which the SCSA® test was used on a rather large cohort of 6881 men classified by age group came to an interesting conclusion [ 71 ]. The authors reported that, as expected and as shown elsewhere with smaller cohorts, DFI increased with age but HDS decreased with age.…”

Section: Discussionmentioning

confidence: 99%

Could high DNA stainability (HDS) be a valuable indicator of sperm nuclear integrity?

Mohammadi

Tavalaee

Gharagozloo

et al. 2020

Basic Clin. Androl.

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Background: The Sperm Chromatin Structure Assay (SCSA®), in addition to identifying the DNA Fragmentation Index (DFI) also identifies High DNA satiability (HDS), supposed to reflect the nuclear compaction of spermatozoa. However, data on what exactly this parameter reveals, its relevance and usefulness are contradictory. In order to shed light on this situation, spermatozoa of a cohort (N = 397) of infertile men were subjected to the SCSA®, TUNEL (terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labeling) and CMA3 (Chromomycin A3) tests. In a smaller subcohort (N = 100), aniline blue (AB) and toluidine blue (TB) staining were performed in addition. The objective of this study was thus to answer the question of whether HDS is a relevant and reliable parameter to be taken into account? Results: HDS does not appear to be a reliable indicator of nuclear immaturity because it shows a weak correlation with the CMA3, AB and TB stains. The low correlation of HDS with sperm DNA fragmentation (TUNEL and SCSA®) and DNA condensation (CMA3, AB and TB) tests suggests that these two parameters could be decoupled. Unlike DFI and TUNEL, HDS has not been shown to correlate with classic clinical situations of male infertility (asthenozoospermia, teratozoospermia or astheno-teratozoospermia). Conclusion: HDS correlates poorly with most tests that focus specifically on the level of maturity of the sperm nucleus. To our knowledge, this study is the first to compare SCSA®, TUNEL, AB, TB and CMA3 assays on identical samples. It shows the potency, consistency and limitations of each test and the care that must be taken in their interpretation.

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“…Deenadayal Mettler et al, (2019) In 6,881 men, significantly higher DFI as measured by SCSA and significantly lower high DNA stainability were observed in men more than 45 years of age compared to younger population (<35, 35-40 and 40-45 years). -Gill, Gill, Jakubik, Fraczek, and Patorski, (2019) In the analysis of ejaculated semen samples from 1,124 men, those older than 40 years had a higher incidence of sperm DNA damage compared to men younger than 40 years.…”

Section: Lifestyle Risk Factors Evidencesmentioning

confidence: 93%

Best laboratory practices and therapeutic interventions to reduce sperm DNA damage

Martínez

Majzoub

2020

Andrologia

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Infertility, defined as the failure to establish a clinical pregnancy after 1 year of regular unprotected sexual intercourse, is considered a worldwide health problem (Zegers-Hochschild, Adamson, Dyer, Racowsky, & de Mouzon, 2017). Globally, 1 out of 6 couples is suffering from failure to achieve clinical pregnancy after 1 year of regular and unprotected sexual intercourse (Sharlip et al., 2002). Poor reproductive potential due to male factor is responsible for 20%-70% of all cases (Agarwal, Mulgund, Hamada, & Chyatte, 2015). Conventional semen analysis is routinely requested to determine the fertility status of men (Barratt, 2007). However, one of the drawbacks of this test is its inability to evaluate advanced sperm functions like sperm deoxyribonucleic acid (DNA) fragmentation (SDF)

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Male age is associated with sperm DNA/chromatin integrity

Cited by 34 publications

References 36 publications

Male age is more critical to sperm DNA integrity than routine semen parameters in Chinese infertile males

Male age is more critical to sperm DNA integrity than routine semen parameters in Chinese infertile males

Could high DNA stainability (HDS) be a valuable indicator of sperm nuclear integrity?

Best laboratory practices and therapeutic interventions to reduce sperm DNA damage

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