Mammalian peptidoglycan recognition protein TagL inhibitsListeria monocytogenesinvasion into epithelial cells

Abstract

Peptidoglycan recognition proteins are a family of evolutionary conserved proteins that play a basic role in the innate immunity of insects, but their role in the immunity of mammals remains unclear. To elucidate its functions, a mouse member of the peptidoglycan recognition proteins family, TagL, was stably expressed in colon adenocarcinoma HT29 cells, and its effect on the invasion and intracellular growth of the enteroinvasive pathogenic bacterium Listeria monocytogenes was assessed. The expression of TagL substantially impaired bacterial invasion and early intracellular growth. The observed effects were partly caused by a loss of viability by intraphagosomal bacteria. Efficient phagosome escaping but not efficient invasion helped bacteria to overplay TagL.

Molecular cloning and functional characterization of peptidoglycan recognition protein 6 in grass carp Ctenopharyngodon idella

“…Invasion and proliferation assay was performed as described in Kibardin et al (2006) with minor modification. 1 Â 10 6 CIK cells were electroporated with 2 lg ptGFP1 or ptGFP1-gcPGRP6 using an AMAXA Nucleofector II transfection system under programme T20.…”

Listeria monocytogenes Infection of Bat Pipistrellus nathusii Epithelial cells Depends on the Invasion Factors InlA and InlB

“…The strains with inlA and inlB deletions were generously provided by Prof. J. Vazquez-Boland, Univ. Edinburgh, UK, and have been used in previous studies [ 50 , 88 ]. Complementation of the inlB deletion with the InlB-expressing plasmid was described earlier [ 50 ].…”

Molecular cloning and functional characterization of the NFIL3/E4BP4 transcription factor of grass carp, Ctenopharyngodon idella

“…Invasion and proliferation assays were performed as previously described (Kibardin et al, 2006) and (Li et al, 2014). Using the LipofectAMINE2000 transfection reagent, 2 × 10 5 CIK cells were transfected with 1 μg pEGFP-N1-gcNFIL3 or pEGFP-N1 plasmids.…”