Molecular cloning and functional characterization of the NFIL3/E4BP4 transcription factor of grass carp, Ctenopharyngodon idella

Yu, Hongyan; Shen, Yubang; Sun, Jun; Xu, Xiaoyan; Wang, Rong-quan; Xuan, Yue; Lu, Liqun; Li, Jiale

doi:10.1016/j.dci.2014.07.019

Cited by 20 publications

(6 citation statements)

References 30 publications

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“…The factor that there was more expression of TRAF6 in survivors of breeding strain compared with those of non-breeding strain, is in line with the resistant related SNP on TRAF6 ( 62 ) and suggests the possible repression on the production of type I IFN ( 63 ). In addition, nuclear factor, interleukin 3 regulated (NFIL3) can control Treg cell function via directly binding to and negatively regulates the expression of Foxp3 ( 64 ), and has been revealed stimulating both proinflammatory (e.g., NF-kappa B [NF-κB]) and anti-inflammatory factors (e.g., IL10) in carp ( 65 ). Thus, the upregulated NFIL3 in the survivors of the breeding strain for group SvC-B may suggest the extensive activation of immune cells, with diminished immune regulation.…”

Section: Discussionmentioning

confidence: 99%

Integrative Transcriptomic Analysis Reveals the Immune Mechanism for a CyHV-3-Resistant Common Carp Strain

Jia

Jiang

et al. 2021

Front. Immunol.

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Anti-disease breeding is becoming the most promising solution to cyprinid herpesvirus-3 (CyHV-3) infection, the major threat to common carp aquaculture. Virus challenging studies suggested that a breeding strain of common carp developed resistance to CyHV-3 infection. This study illustrates the immune mechanisms involved in both sensitivity and anti-virus ability for CyHV3 infection in fish. An integrative analysis of the protein-coding genes and long non-coding RNAs (lncRNAs) using transcriptomic data was performed. Tissues from the head kidney of common carp were extracted at days 0 (the healthy control) and 7 after CyHV-3 infection (the survivors) and used to analyze the transcriptome through both Illumina and PacBio sequencing. Following analysis of the GO terms and KEGG pathways involved, the immune-related terms and pathways were merged. To dig out details on the immune aspect, the DEGs were filtered using the current common carp immune gene library. Immune gene categories and their corresponding genes in different comparison groups were revealed. Also, the immunological Gene Ontology terms for lncRNA modulation were retained. The weighted gene co-expression network analysis was used to reveal the regulation of immune genes by lncRNA. The results demonstrated that the breeding carp strain develops a marked resistance to CyHV-3 infection through a specific innate immune mechanism. The featured biological processes were autophagy, phagocytosis, cytotoxicity, and virus blockage by lectins and MUC3. Moreover, the immune-suppressive signals, such as suppression of IL21R on STAT3, PI3K mediated inhibition of inflammation by dopamine upon infection, as well as the inhibition of NLRC3 on STING during a steady state. Possible susceptible factors for CyHV-3, such as ITGB1, TLR18, and CCL4, were also revealed from the non-breeding strain. The results of this study also suggested that Nramp and PAI regulated by LncRNA could facilitate virus infection and proliferation for infected cells respectively, while T cell leukemia homeobox 3 (TLX3), as well as galectin 3 function by lncRNA, may play a role in the resistance mechanism. Therefore, immune factors that are immunogenetically insensitive or susceptible to CyHV-3 infection have been revealed.

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Section: Discussionmentioning

confidence: 99%

Integrative Transcriptomic Analysis Reveals the Immune Mechanism for a CyHV-3-Resistant Common Carp Strain

Jia

Jiang

et al. 2021

Front. Immunol.

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“…Primers were designed according to the RNA-Seq results, using Primer Premier 5 (Premier, Canada) ( S1 Table ) [ 43 ]. The arithmetic mean value of the 18S rRNA [ 44 – 46 ] and β -actin [ 47 – 49 ] genes of grass carp served as internal control value, to normalize the expression levels. After total RNA from each sample at each time point was extracted, reverse transcription was used to synthesize cDNA using the PrimeScript ™ RT reagent Kit with gDNA Eraser (Takara, Shanghai, China), according to the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

Transcriptome Analysis of the Innate Immunity-Related Complement System in Spleen Tissue of Ctenopharyngodon idella Infected with Aeromonas hydrophila

Dang

Shen

et al. 2016

PLoS ONE

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The grass carp (Ctenopharyngodon idella) is an important commercial farmed herbivorous fish species in China, but is susceptible to Aeromonas hydrophila infections. In the present study, we performed de novo RNA-Seq sequencing of spleen tissue from specimens of a disease-resistant family, which were given intra-peritoneal injections containing PBS with or without a dose of A. hydrophila. The fish were sampled from the control group at 0 h, and from the experimental group at 4, 8, 12, 24, 48 and 72 h. 122.18 million clean reads were obtained from the normalized cDNA libraries; these were assembled into 425,260 contigs and then 191,795 transcripts. Of those, 52,668 transcripts were annotated with the NCBI Nr database, and 41,347 of the annotated transcripts were assigned into 90 functional groups. 20,569 unigenes were classified into six main categories, including 38 secondary KEGG pathways. 2,992 unigenes were used in the analysis of differentially expressed genes (DEGs). 89 of the putative DEGs were related to the immune system and 41 of them were involved in the complement and coagulation cascades pathway. This study provides insights into the complement and complement-related pathways involved in innate immunity, through expression profile analysis of the genomic resources in C. idella. We conclude that complement and complement-related genes play important roles during defense against A. hydrophila infection. The immune response is activated at 4 h after the bacterial injections, indicating that the complement pathways are activated at the early stage of bacterial infection. The study has improved our understanding of the immune response mechanisms in C. idella to bacterial pathogens.

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“…The ORF of Ctenopharyngodon idella tlr5 ( Citlr5 ) was amplified from grass carp cDNA and individually cloned into the pEGFP-N1 vector (Promega) by directional cloning. Invasion and proliferation assays were performed as previously described 17 . Using the Lipofectamin 2000 (Invitrogen) transfection reagent, 2 × 10 5 Ctenopharyngodon idella kidney (CIK) cells cellswere transfected with 1 μg pEGFP-N1-CiTLR5 plasmids or 50 nM cid-miRn-115 and miR-142a-3p agomir (GenePharm, China).…”

Section: Methodsmentioning

confidence: 99%

MicroRNA-induced negative regulation of TLR-5 in grass carp, Ctenopharyngodon idella

Shen

et al. 2016

Sci Rep

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MicroRNAs (miRNAs) are endogenous small non-coding RNAs that play crucial roles in numerous biological processes. However, the role of miRNAs in antibacterial defence in fish has not been fully determined. Here, we identified that nine miRNAs are differentially expressed in kidney between susceptible and resistant grass carp strains. Analysis of spatial and temporal miRNA expression patterns suggests that cid-miRn-115 and miR-142a-3p are potential regulators of anti-bacterial activity. Overexpressing of cid-miRn-115 and miR-142a-3p results in a visible change in Ctenopharyngodon idella kidney (CIK) cells immune effector activity. Bioinformatics analysis and overexpressing assay shows that cid-miRn-115 and miR-142a-3p directly regulate tlr5 expression. cid-miRn-115 and miR-142a-3p overexpressing leads to a significant decrease in tlr5 expression in CIK, thereby repressing its downstream genes, such as il-1β, il-8 and tnf-α. These findings provide a novel insight into the determination of anti-bacterial compounds in grass carp.

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Molecular cloning and functional characterization of the NFIL3/E4BP4 transcription factor of grass carp, Ctenopharyngodon idella

Cited by 20 publications

References 30 publications

Integrative Transcriptomic Analysis Reveals the Immune Mechanism for a CyHV-3-Resistant Common Carp Strain

Integrative Transcriptomic Analysis Reveals the Immune Mechanism for a CyHV-3-Resistant Common Carp Strain

Transcriptome Analysis of the Innate Immunity-Related Complement System in Spleen Tissue of Ctenopharyngodon idella Infected with Aeromonas hydrophila

MicroRNA-induced negative regulation of TLR-5 in grass carp, Ctenopharyngodon idella

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