MAP-kinase activity in etiolated Cucumis sativus cotyledons: The effect of red and far-red light irradiation

Alvarez-Flórez, Fagua; Vidal, D.; Simón, Esther

doi:10.1016/j.plaphy.2012.11.008

Cited by 12 publications

(5 citation statements)

References 35 publications

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“…A total of 65 MAPK cascade genes were identified in Tartary buckwheat and classified into three families: MAPK, MAPKK, and MAPKKK, with 8 in the MAPK family, the fewest MAPKK members (n = 1), and 56 MAPKKK genes. This distribution is consistent with that found in other plants; for example, there are 20, 10, and 80 MAPK, MAPKK, and MAPKKK genes, respectively, in A. thaliana ( Colcombet and Hirt, 2008 ) ( Alvarez-Flórez et al, 2013 ), and 12, 7, and 73 in F. vesca ( Alvarez-Flórez et al, 2013 ) ( Heying et al, 2017 ).…”

Section: Discussionsupporting

confidence: 88%

Genome-Wide Identification of MAPK, MAPKK, and MAPKKK Gene Families in Fagopyrum tataricum and Analysis of Their Expression Patterns Under Abiotic Stress

et al. 2022

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The mitogen-activated protein kinase (MAPK) cascade is a highly conserved signal transduction pathway, ubiquitous in eukaryotes, such as animals and plants. The MAPK cascade has a dominant role in regulating plant adaptation to the environment, such as through stress responses, osmotic adjustment, and processes that modulate pathogenicity. In the present study, the MAPK cascade gene family was identified in Fagopyrum tataricum (Tartary buckwheat), based on complete genome sequence data. Using phylogenetic tree, conservative motif, and chromosome location analyses, a total of 65 FtMAPK cascade genes, distributed on five chromosomes, were classified into three families: MAPK (n = 8), MAPKK (n = 1), and MAPKKK (n = 56). Transcriptome data from Tartary buckwheat seedlings grown under different light conditions demonstrated that, under blue and red light, the expression levels of 18 and 36 FtMAPK cascade genes were up-regulated and down-regulated, respectively. Through qRT-PCR experiments, it was observed that FtMAPK5, FtMAPKK1, FtMAPKKK8, FtMAPKKK10, and FtMAPKKK24 gene expression levels in the Tartary buckwheat seedlings increased under three types of abiotic stress: drought, salt, and high temperature. A co-expression network of FtMAPK cascade genes was constructed, based on gene expression levels under different light conditions, and co-expressed genes annotated by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses, which identified numerous transcription factors related to plant abiotic stress. The authors conclude that FtMAPK cascade genes have important roles in the growth and development of Tartary buckwheat, as well as its responses to abiotic stress.

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Section: Discussionsupporting

confidence: 88%

Genome-Wide Identification of MAPK, MAPKK, and MAPKKK Gene Families in Fagopyrum tataricum and Analysis of Their Expression Patterns Under Abiotic Stress

et al. 2022

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“…An increasing number of studies have highlighted the involvement of MAPK cascades in regulating plant light responses. In cucumber ( Cucumis sativus ), two MAPK proteins are activated and involved in far‐red‐ and red‐light‐induced etiolation (Alvarez‐Flórez et al , 2013). Light can activate MPK3, MPK4 and MPK6 in A. thaliana (Lee, 2015); the A. thaliana MKK3–MPK6 cascade responds to blue light treatment and participates in hypocotyl growth by phosphorylating MYC2 (Sethi et al , 2014), and phosphorylation of phytochrome‐interacting factor 3 (PIF3) by MKK10–MPK6 modulates red‐light‐induced cotyledon opening of seedlings (Xin et al , 2018).…”

Section: Discussionmentioning

confidence: 99%

Apple MPK4 mediates phosphorylation of MYB1 to enhance light‐induced anthocyanin accumulation

Yang

et al. 2021

The Plant Journal

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Anthocyanins are plant pigments with diverse biological functions that contribute to fruit quality and are beneficial to human health. Anthocyanin accumulation can be influenced by environmental signals, such as light, and plants have developed sophisticated systems to receive and transduce these signals. However, the associated molecular mechanisms are not well understood. In this study, we investigated the potential function of mitogen-activated protein kinases, which are members of the light signaling pathway, during light-induced anthocyanin accumulation in apple (Malus domestica) fruit peels. An antibody array and yeast two-hybrid screen indicated that proteins encoded by two MdMPK4 genes are light-activated and interact with the transcription factor and anthocyanin biosynthesis regulator MdMYB1. A phosphorylation assay showed that the MdMPK4 proteins phosphorylate MdMYB1, thereby increasing its stability under light conditions. Transient MdMPK4 and MdMYB1 overexpression assays further revealed that light-induced anthocyanin accumulation relies on MdMPK4 kinase activity, which is required for maximum MdMYB1 activity. Based on the expression of the chromosome 6 allele MdMPK4-06G under light conditions and the presence of light response elements in the MdMPK4-06G promoter, we concluded that it is more responsive to light than the chromosome 14 allele MdMPK4-14G. These results suggest a potential biotechnological strategy for increasing fruit anthocyanin content via light induction.

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“…MAPK cascades in plants play important roles in regulating growth, development, and stress responses. Although the role of MAPK cascades in regulating plant light responses is far from clear, several lines of evidence suggest that they are involved in this process: two MAPKs in cucumber ( Cucumis sativus ) cotyledons are activated during FR- and R-light-induced seedling de-etiolation ( Alvarez-Flórez et al , 2013 ); the transcription of several MAPK cascade genes in Arabidopsis is regulated by light, and among these, MKKK14 is up-regulated by R light and its null mutant shows a short-hypocotyl phenotype in FR- but not R-light-induced seedling de-etiolation ( Khanna et al , 2006 ; López-Juez et al , 2008 ; Tepperman et al , 2006 ); and the Arabidopsis MKK3-MPK6 cascade is activated by blue (B) light and regulates hypocotyl growth through phosphorylation of MYC2 ( Sethi et al , 2014 ). Arabidopsis contains 60 MAPKKKs, 10 MAPKKs, and 20 MAPKs (nominated as MKKKs, MKKs, and MPKs, respectively) ( MAPK Group, 2002 ).…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis MKK10-MPK6 mediates red-light-regulated opening of seedling cotyledons through phosphorylation of PIF3

Xin

Chen

Wang

et al. 2017

Journal of Experimental Botany

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MAP-kinase activity in etiolated Cucumis sativus cotyledons: The effect of red and far-red light irradiation

Cited by 12 publications

References 35 publications

Genome-Wide Identification of MAPK, MAPKK, and MAPKKK Gene Families in Fagopyrum tataricum and Analysis of Their Expression Patterns Under Abiotic Stress

Genome-Wide Identification of MAPK, MAPKK, and MAPKKK Gene Families in Fagopyrum tataricum and Analysis of Their Expression Patterns Under Abiotic Stress

Apple MPK4 mediates phosphorylation of MYB1 to enhance light‐induced anthocyanin accumulation

Arabidopsis MKK10-MPK6 mediates red-light-regulated opening of seedling cotyledons through phosphorylation of PIF3

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