Mass Propagation of New Gladiolus Hybrids

Lilien-Kipnis, H.; Kochba, M.

doi:10.17660/actahortic.1987.212.105

Cited by 18 publications

(17 citation statements)

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“…No reference could however be found in the literature on the effect of temperature on in vitro corm formation in Gladiolus. Other investigators kept the cultures at 23 ° to 25 °C (Ziv et al 1970;Bajaj et al 1983;Dantu & Bhojwani 1987;Lilien-Kipnis & Kochba 1987;Ziv 1989) or even 26 ° to 29°C (Simonsen & Hildebrandt 1971). Taeb & Alderson (1990) however illustrated that a lowtemperature incubation of shoot cultures of tulips enhanced bulblet development and that it was associated with a high level of sucrose in the shoots.…”

Section: Discussionmentioning

confidence: 99%

“…The explants were cultured at 24---2 °C and a 16h light/8h dark photoperiod. The experiments were conducted at 24 °C as this temperature was recommended by most of the previous authors (Ziv et al 1970;Bajaj et al 1983;Dantu & Bhojwani 1987;Lilien-Kipnis & Kochba 1987;Ziv 1989;Steinitz et al 1991). Each treatment consisted of 20 repetitions.…”

Section: Methodsmentioning

confidence: 99%

“…Rapid in vitro propagation techniques have been reported for several Gladiolus hybrids (Ziv et al 1970;Simonsen & Hildebrandt 1971;Wilfret 1971;Hussey 1977;Ziv 1979;Bajaj et al 1983;Logan & Zettler 1985;Sutter 1986;Dantu & Bhojwani 1987;Lilien-Kipnis & Kochba 1987;Ziv 1989;Steinitz et al 1991). Although the first shoots were generated from inflorescence stalks (Ziv et al 1970) the best results are now achieved with axillary buds on solid and liquid media (Dantu & Bhojwani 1987;Lilien-Kipnis & Kochba 1987;Ziv 1989;Steinitz et al 1991).…”

Section: Introductionmentioning

confidence: 99%

“…Although the first shoots were generated from inflorescence stalks (Ziv et al 1970) the best results are now achieved with axillary buds on solid and liquid media (Dantu & Bhojwani 1987;Lilien-Kipnis & Kochba 1987;Ziv 1989;Steinitz et al 1991). As transplanting and establishing of in vitro produced Gladiolus plantlets were not always easy, attempts were made to produce in vitro corms from shoots developing from the axillary buds (Dantu & Bhojwani 1987).…”

Section: Introductionmentioning

confidence: 99%

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In vitro corm production of Gladiolus dalenii and G. tristis

Bruyn¹,

Ferreira²

1992

Plant Cell Tiss Organ Cult

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The ability of the summer flowering Gladiolus dalenii Van Geel and the winter flowering G. tristis L. to form corms in vitro was investigated. G. dalenii spontaneously formed corms on a shoot induction medium consisting of the basal medium of Murashige & Skoog (1962) with up to 2.0mg1-1 benzyladenine (BA), 3% sucrose and solidified with 2 g l 1 Gelrite ®. The effect of different BA and sucrose concentrations as well as different temperatures on in vitro corm production of G. tristis was further investigated. The best production of shoots per explant was achieved on a medium containing 0.5 to 1.0mg1-1 BA, sucrose concentrations of 6 to 9% and cultured at 15°C. The best corm production was achieved at the same temperature and with the same medium with the exception that BA was omitted from the medium. To test the effect of the osmotic potential on the formation of shoots and corms, sucrose was substituted by mannitol at various concentrations. Sucrose proved to be essential for both shoot and corm production and the use of mannitol had no beneficial effect.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In vitro corm production of Gladiolus dalenii and G. tristis

Bruyn¹,

Ferreira²

1992

Plant Cell Tiss Organ Cult

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“…After adjusting the pH of media to 5.8, gelrite was added at 2.5 g/l prior to autoclaving. Culture conditions were adjusted for 16 hr photoperiod at 23±2EC in accordance with the recommendations (Ziv et al, 1970;Bajaj, 1983;Lilien-Kipnis and Kochba 1987;Steinitz et al, 1991). Each treatment consisted of 25 replications.…”

Section: Methodsmentioning

confidence: 99%

In vitro Production of Cormlets in Gladiolus

Ahmad¹,

Ahmad²,

Nasir³

et al. 2000

Pakistan J. of Biological Sciences

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Abstract:The ability of explants of gladiolus cv White Prosperity to form plantlets and cormlets was investigated in vitro studies. Maximum production of shoots per explant was obtained on basal medium of Murashige and Skoog (1962) containing 18 :M 6-Benzylaminopurin (BAP) and 36 :M Indole-3-butyric Acid (IBA). Callus formation was observed when in vitro cultures were maintained for 16-18 weeks. When different explants were used for their response to regenerate, maximum number of shoots per culture was achieved from nodal buds. In order to allow initiation of root formation, the shoots were transferred to one half strength Murashige and Skoog medium containing 2.5 :M, Naphthalenacetic Acid (NAA) or 19 :M Indole -3-butyric Acid (IBA). Roots developed readily within 4-5 weeks on either of the two media. However, all plantlets grew with better survival in medium containing NAA as harmonal supplement than in media containing IBA. Further MS medium containing 6 percent sucrose facilitated maximum growth of cormlets to allow their use as propagation material in vivo.

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