Mass spectrometry of pertrimethylsilyl aldosyl oligosaccharides

Abstract

Abatraet-The mass spectra of 18 trimethylsilyl disaccharides containing only aldohexoses, connected via all possible linkages (I d 1) to (1 -+ 6), were compared. These spectra could be divided into three main groups: (1 + 1) disaccharides, (1 + 2), (1 -+ 3), (1 +'4) disaccharides and (1 -+ 5), (I + 6) disaccharides.(t + Zf, (I -+ 3) and (1 -+ 4) disaccharides could be distinguished on the basis of two ratios of peak intensities. (i + 5) and (1 -+ 6) disaccharides could not be distinguished. Also, the mass spectra of 4 trimethylsilyl trisaccharides built up from aldohexose-s only were recorded. The spectra of three aldopentose containing disaccharides showed that the monosaccharide sequence as well as the position of the glycosidic link could be determined.

Mass Spectrometric Fragmentation of Trimethylsilyl and Related Alkylsilyl Derivatives

“…However, Kamerling et al () have found that the ion at m/z 683 was also present in the spectra of disaccharides with 1→5 (furanose form) and 1→6‐linkages and that the peak at m/z 668 was also detectable in the spectra of the 1→3 linked compounds, although its intensity was low. Peaks were not found to differentiate the 1→5 and 1→6‐linkages.…”

Mass spectral studies of some fructose containing oligosaccharide permethyl ethers

“…6 The known major contributions to the formation of an m/e 101 ion are shown below.1.10*11*12GLUCOPYRANOSE FR U CTO FUR ANOSE.Similarly the genesis of m/e 88 from the known major processes is indicated bel0~.1.…”

Characterization ofO-trimethylsilyl oximes of disaccharides by gas chromatography-mass spectrometry

“…The derivatization procedure described previously gives for these compounds single peaks of their octakis-TMS ethers, whose corresponding mass spectra have already been described [12,13]. They showed characteristic ions at m/z 435,437 and 451.…”