Materials from Mussel-Inspired Chemistry for Cell and Tissue Engineering Applications

Abstract

Current advances in biomaterial fabrication techniques have broadened their application in different realms of biomedical engineering, spanning from drug delivery to tissue engineering. The success of biomaterials depends highly on the ability to modulate cell and tissue responses, including cell adhesion, as well as induction of repair and immune processes. Thus, most recent approaches in the field have concentrated on functionalizing biomaterials with different biomolecules intended to evoke cell- and tissue-specific reactions. Marine mussels produce mussel adhesive proteins (MAPs), which help them strongly attach to different surfaces, even under wet conditions in the ocean. Inspired by mussel adhesiveness, scientists discovered that dopamine undergoes self-polymerization at alkaline conditions. This reaction provides a universal coating for metals, polymers, and ceramics, regardless of their chemical and physical properties. Furthermore, this polymerized layer is enriched with catechol groups that enable immobilization of primary amine or thiol-based biomolecules via a simple dipping process. Herein, this review explores the versatile surface modification techniques that have recently been exploited in tissue engineering and summarizes polydopamine polymerization mechanisms, coating process parameters, and effects on substrate properties. A brief discussion of polydopamine-based reactions in the context of engineering various tissue types, including bone, blood vessels, cartilage, nerves, and muscle, is also provided.

Nano-silver-decorated microfibrous eggshell membrane: processing, cytotoxicity assessment and optimization, antibacterial activity and wound healing

“…3G); (3) DA is biocompatible and relatively nontoxic to the human body (Fig. 2J); and (4) the assembly process of AgNPs is simple and mild at room temperature 33, 40 .…”

“…Inspired by mussel adhesiveness, Messersmith and his colleagues found that dopamine (DA) can undergo oxidative self-polymerization under alkaline conditions to form polydopamine (PD), enabling biomolecular or cellular attachment, and the catechol group in PD has the ability for chemical reduction 33, 34 . The mussel-inspired DA has super adhesive activity and can create a polymerized layer on various surfaces of material 31, 35 .…”

Facile Cell Sheet Harvest and Translocation Mediated by a Thermally Expandable Hydrogel with Controlled Cell Adhesion

“…[13][14][15] Deposition of PD onto biomaterials (pH = 8.5) is irrespective of their surface chemistry, which accelerated its application onto various substrates from metal to synthetic polymer used in tissue engineering and biochip approaches. [16][17][18] However, majority of these works involved functionalization of rigid and solid materials, and a limited number of studies have been available on soft materials such as hydrogels. [19] In addition, tuning dopamine coating on the hydrogels for modulating attachment and detachment of multiple cell types as a cell sheet has yet to be done.…”

“…Generally, PD coating has been conducted by a single step on solid biomaterials such as TiO 2 and poly(lactic-co-glycolic acid) (PLGA). [17] Prolonged incubation of biomaterials within PD leads to the deposition of greater amounts of PD, and enhanced cell adhesion likely in a PD-concentration-dependent manner. [23,24] However, the harvest and translocation of the cell sheet requires a balance between cell attachment and detachment.…”

Imitation‐mussel‐based high‐performance conductive coating on hydrophobic fabric for thermochromic application

“…At the same time, PDA can be used as a platform for grafting various functional molecules to obtain surface‐functionalized materials . It also can be used alone as a matrix material to obtain new materials with special functions . Until now, the applications of PDA have mainly been focused on the preparation of the adhesive, wettability regulation, metallization–inorganic mineralization, bioactivity, and antifouling antibacterial surfaces .…”