Matrix metalloproteinases, their physiological inhibitors and osteoclast factors are differentially regulated by the cytokine profile in human periodontal disease

Garlet, Gustavo; Martins, Wilson; Fonseca, Benedito Antônio Lopes da; Ferreira, Beatriz Rossetti; Silva, João

doi:10.1111/j.1600-051x.2004.00545.x

Cited by 191 publications

(275 citation statements)

References 59 publications

(73 reference statements)

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“…Furthermore, the increased ePD severity correlates with the significant increase in the levels of RANKL and MMP-13, and with the increase of RANKL/OPG and MMP-13/TIMPs ratios, characteristically associated with periodontal destruction. 13,19,45 Looking for the reason of the altered adaptive response because of RA/ PD interaction, we found that the submandibular LNs (the major draining LNs of oral cavity), which are significantly enlarged by the ePD induction, presented an even higher enlargement because of the pristane injection (data not shown), being an indicative of systemic immune reactivity to the PIA. Furthermore, the pattern of the transcription factors responsible for T-cell polarization expressed in submandibular LN was modulated by the PIA, the levels of Tbet and RORg being significantly increased in AIRmax mice submitted to both PIA and ePD protocols.…”

Section: Experimental Periodontitis and Arthritis Interaction Ap Trommentioning

confidence: 90%

Periodontitis and arthritis interaction in mice involves a shared hyper-inflammatory genotype and functional immunological interferences

Trombone¹,

Claudino²,

Colavite³

et al. 2010

Genes Immun

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Periodontitis (PD) and rheumatoid arthritis (RA) have been found to be clinically associated and to share the chronic nature of the inflammatory reaction associated with bone resorption activity. However, the mechanisms underlying such association are unknown. Therefore, we examined the basis of Actinobacillus actinomycetemcomitans-and Porphyromonas gingivalis-induced PD and pristane-induced arthritis (PIA) interaction in mice. Higher severity PD in the genetically inflammation prone acute inflammatory reactivity maximum (AIRmax) mice strain was associated with higher levels of TNF-a, IL-1b, IL-17, matrix metalloproteinase (MMP)-13, and RANKL, whereas PD/PIA co-induction resulted in even higher levels of IL-1b, IFN-g, IL-17, RANKL, and MMP-13 levels. Conversely, PD/PIA co-induction in AIRmin strain did not alter the course of both pathologies. PIA/PD co-induction resulted in altered expression of T-cell subsets transcription factors expression, with T-bet and RORg levels being upregulated, whereas GATA-3 levels were unaltered. Interestingly, PIA induction resulted in alveolar bone loss, such response being highly dependent on the presence of commensal oral bacteria. No differences were found in PIA severity parameters by PD co-induction. Our results show that the interaction between experimental PD and arthritis in mice involves a shared hyper-inflammatory genotype and functional interferences in innate and adaptive immune responses.

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Section: Experimental Periodontitis and Arthritis Interaction Ap Trommentioning

confidence: 90%

Periodontitis and arthritis interaction in mice involves a shared hyper-inflammatory genotype and functional immunological interferences

Trombone¹,

Claudino²,

Colavite³

et al. 2010

Genes Immun

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“…ECM turnover is clearly involved during bone remodeling and matrix metalloproteinases (MMPs) govern these events (7). MMPs are zinc-dependent endopeptidases.…”

Section: Fluoride Modulates Preosteoblasts Viability Andmentioning

confidence: 99%

Fluoride modulates preosteoblasts viability and matrix metalloproteinases-2 and -9 activities

et al. 2012

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This study evaluated the influence of fluoride on cell viability and activity of matrix metalloproteinases (MMP) -2 and -9 secreted by preosteoblasts. Preosteoblasts (MC3T3-E1 murine cell line) were cultured in MEM medium supplement with 10% Fetal Bovine Serum (FBS) and nucleosides/ribonucleosides without ascorbic acid. Adherent cells were treated with different concentrations of F (as sodium fluoride-NaF) in medium (5 x 10 -6 M, 10 -5 M, 10 -4 M and 10 -3 M) for 24, 48, 72 and 96 h at 37 o C, 5% CO 2 . Control cells were cultivated in MEM only. After each period, preosteoblast viability was assessed by MTT assay. MMP-2 and -9 activities were performed by gel zymography. Also, alkaline phosphatase (ALP) activity was quantified by colorimetry in all experimental groups. It was shown that cultured cells with the highest dose of F (10 -3 M) for 96 h decreased preosteoblast viability while lower doses of F did not alter it, when compared to untreated cells. No differences were observed in ALP activity among groups. Moreover, compared to control, the treatment of cells with F at low dose slightly increased MMP-2 and -9 activities after 24 h. It was concluded that F modulates preosteoblast viability in a dose-dependent manner and also may regulate extracellular matrix remodeling.

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“…Cytokines such as interleukin-1, tumor necrosis factor-, prostaglandin E2, and more recently adipokines, leptin, have been shown to orchestrate the host response to infectious and inflammatory stimuli. 1 Hence, the present study was designed to assess the concentration of human leptin in gingival crevicular fluid (GCF) and serum and to find out their link, if any, in periodontal health and disease; further, to get deeper insight into its possible role in commencement and development of periodontal disease.…”

Section: 5005/jp-journals-10029-1023mentioning

confidence: 99%

An Evaluation and Correlation of Leptin in Gingival Crevicular Fluid and Serum in Health, Gingivitis and Periodontitis

Vadvadgi¹,

Padmawar²

2012

International Journal of Experimental Dental Science

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Background and objective: Plasma leptin is associated in patients with inflammatory diseases. A high concentration of leptin is associated with healthy gingival tissue. The purpose of this study was to assess the concentration of human leptin in gingival crevicular fluid (GCF) and serum within healthy and diseased gingiva, further to explore the possibility of using the levels of leptin in GCF and serum as a biochemical marker of periodontal disease progression.

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Matrix metalloproteinases, their physiological inhibitors and osteoclast factors are differentially regulated by the cytokine profile in human periodontal disease

Cited by 191 publications

References 59 publications

Periodontitis and arthritis interaction in mice involves a shared hyper-inflammatory genotype and functional immunological interferences

Periodontitis and arthritis interaction in mice involves a shared hyper-inflammatory genotype and functional immunological interferences

Fluoride modulates preosteoblasts viability and matrix metalloproteinases-2 and -9 activities

An Evaluation and Correlation of Leptin in Gingival Crevicular Fluid and Serum in Health, Gingivitis and Periodontitis

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