The purpose of this study was to describe the physiological requirements of a 2000 m simulated rowing performance, and to examine the relationship between this performance and various physical and physiological parameters. The measurements made on 22 female and 10 male rowers included a 2000 m simulated rowing performance, height, body mass, ventilatory threshold (VT), power output at VT (PO at VT), heart rate at VT, absolute maximal oxygen consumption (VO 2 max), power output at VO 2 max (PO at VO 2 max), and leg and bench press one repetition maximum (1 RM). A 2000 m simulated rowing test was performed at a mean intensity of 90.2% of VO 2 max, 77.0% of the PO at VO 2 max, and at 95.8% of HRmax. Significant correlation was found between the 2000 m rowing time and a competitor's height (r= -0.83), body mass (r = -0.69), VT (r = -0.81), PO at VT (r=-0.74), VO 2 max (r = -0.96), PO at VO 2 max (r = -0.83) and all strength variables (r = -0.56 to -0.79), respectively. Stepwise multiple regression indicated that the absolute VO 2 max was the best predictor of 2000 m simulated rowing performance (p 0.05). It was also found that males utilize strength to a greater degree than female to perform the 2000 m simulated rowing performance trial.