Measuring Mass of Nanoparticles and Viruses in Liquids with Nanometer-Scale Pores

Arjmandi, Nima; Roy, W. Van; Lagae, Liesbet

doi:10.1021/ac500396t

Cited by 28 publications

(28 citation statements)

References 36 publications

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“…The characteristics of resistive pulse, the amplitude and duration, can therefore be used to describe the extent of viral particle deformation. While several groups have used nanopores for characterization of biological nanoparticles, such as exosomes and viruses , the possible deformation of these particles inside nanopores and the resultant effects on particle characterization have been widely neglected.…”

Section: Introductionmentioning

confidence: 99%

Mechanical characterization of HIV‐1 with a solid‐state nanopore sensor

et al. 2018

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Enveloped viruses fuse with cells to transfer their genetic materials and infect the host cell. Fusion requires deformation of both viral and cellular membranes. Since the rigidity of viral membrane is a key factor in their infectivity, studying the rigidity of viral particles is of great significance in understating viral infection. In this paper, a nanopore is used as a single molecule sensor to characterize the deformation of pseudo-type human immunodeficiency virus type 1 at sub-micron scale. Non-infective immature viruses were found to be more rigid than infective mature viruses. In addition, the effects of cholesterol and membrane proteins on the mechanical properties of mature viruses were investigated by chemically modifying the membranes. Furthermore, the deformability of single virus particles was analyzed through a recapturing technique, where the same virus was analyzed twice. The findings demonstrate the ability of nanopore resistive pulse sensing to characterize the deformation of a single virus as opposed to average ensemble measurements.

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Section: Introductionmentioning

confidence: 99%

Mechanical characterization of HIV‐1 with a solid‐state nanopore sensor

et al. 2018

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“…Because the platform ensures the detection of a single particle, it is possible to detect a variety of such particles in a mixed solution, one by one 48 . Conventional Raman spectroscopy of a solution consisting of different particles would result in an incomprehensible signal of peaks from all particles.…”

Section: Discussionmentioning

confidence: 99%

Raman fingerprinting of single dielectric nanoparticles in plasmonic nanopores

Kerman

Chang

et al. 2015

Nanoscale

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Plasmonic nano-apertures are commonly used for the detection of small particles such as nanoparticles and proteins by exploiting electrical and optical techniques. Plasmonic nanopores are metallic nano-apertures sitting on a thin membrane with a tiny hole. It has been shown that plasmonic nanopores with a given geometry identify internal molecules using Surface Enhanced Raman Spectroscopy (SERS). However, label-free identification of a single dielectric nanoparticle requires a highly localized field comparable to the size of the particle. Additionally, the particle's Brownian motion can jeopardize the amount of photons collected from a single particle. Here, we demonstrate that the combination of optical trapping and SERS can be used for the detection and identification of 20 nm polystyrene nanoparticles in plasmonic nanopores. This work is anticipated to contribute to the detection of small bioparticles, optical trapping and nanotribology studies.

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“…Notably, the surface of this nanopore was covalently modified with triethylene glycol to minimize capsid adsorption and suppress electroosmotic flow within the pore. Arjmandi and coworkers constructed pyramidal-shaped pores as shown in Figure 2B to detect human immunodeficiency virus and Epstein-Barr virus (Arjmandi et al, 2012 , 2014 ). They fabricated pores of 20–500 nm in size on a silicon membrane using electron beam lithography followed by anisotropic wet etching using potassium hydroxide.…”

Section: Applications Of Rps For Virus Detectionmentioning

confidence: 99%

Quantification of Virus Particles Using Nanopore-Based Resistive-Pulse Sensing Techniques

Yang

Yamamoto

2016

Front. Microbiol.

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Viruses have drawn much attention in recent years due to increased recognition of their important roles in virology, immunology, clinical diagnosis, and therapy. Because the biological and physical properties of viruses significantly impact their applications, quantitative detection of individual virus particles has become a critical issue. However, due to various inherent limitations of conventional enumeration techniques such as infectious titer assays, immunological assays, and electron microscopic observation, this issue remains challenging. Thanks to significant advances in nanotechnology, nanostructure-based electrical sensors have emerged as promising platforms for real-time, sensitive detection of numerous bioanalytes. In this paper, we review recent progress in nanopore-based electrical sensing, with particular emphasis on the application of this technique to the quantification of virus particles. Our aim is to provide insights into this novel nanosensor technology, and highlight its ability to enhance current understanding of a variety of viruses.

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Measuring Mass of Nanoparticles and Viruses in Liquids with Nanometer-Scale Pores

Cited by 28 publications

References 36 publications

Mechanical characterization of HIV‐1 with a solid‐state nanopore sensor

Mechanical characterization of HIV‐1 with a solid‐state nanopore sensor

Raman fingerprinting of single dielectric nanoparticles in plasmonic nanopores

Quantification of Virus Particles Using Nanopore-Based Resistive-Pulse Sensing Techniques

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