Members of 14-3-3 protein isoforms interacting with the resistance gene product N and the elicitor of Tobacco mosaic virus

Konagaya, Ken Ichi; Matsushita, Yasuhiko; Kasahara, Masahiro; Nyunoya, Hiroshi

doi:10.1007/s10327-003-0113-4

Cited by 63 publications

(56 citation statements)

References 46 publications

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“…S5). Previous studies have highlighted the role of 14-3-3 proteins during ETI (Konagaya et al, 2004;Yang et al, 2009;Oh et al, 2010;Oh and Martin, 2011). It is possible that HopQ1's virulence-promoting effect during ETI may be due to interfering with 14-3-3 proteins necessary for robust ETI signaling.…”

Section: Discussionmentioning

confidence: 99%

The Pseudomonas syringae Effector HopQ1 Promotes Bacterial Virulence and Interacts with Tomato 14-3-3 Proteins in a Phosphorylation-Dependent Manner

Yadeta

Elmore

et al. 2013

Plant Physiology

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A key virulence strategy of bacterial pathogens is the delivery of multiple pathogen effector proteins into host cells during infection. The Hrp outer protein Q (HopQ1) effector from Pseudomonas syringae pv tomato (Pto) strain DC3000 is conserved across multiple bacterial plant pathogens. Here, we investigated the virulence function and host targets of HopQ1 in tomato (Solanum lycopersicum). Transgenic tomato lines expressing dexamethasone-inducible HopQ1 exhibited enhanced disease susceptibility to virulent Pto DC3000, the Pto ƊhrcC mutant, and decreased expression of a pathogen-associated molecular pattern-triggered marker gene after bacterial inoculation. HopQ1-interacting proteins were coimmunoprecipitated and identified by mass spectrometry. HopQ1 can associate with multiple tomato 14-3-3 proteins, including TFT1 and TFT5. HopQ1 is phosphorylated in tomato, and four phosphorylated peptides were identified by mass spectrometry. HopQ1 possesses a conserved mode I 14-3-3 binding motif whose serine-51 residue is phosphorylated in tomato and regulates its association with TFT1 and TFT5. Confocal microscopy and fractionation reveal that HopQ1 exhibits nucleocytoplasmic localization, while HopQ1 dephosphorylation mimics exhibit more pronounced nuclear localization. HopQ1 delivered from Pto DC3000 was found to promote bacterial virulence in the tomato genotype Rio Grande 76R. However, the HopQ1(S51A) mutant delivered from Pto DC3000 was unable to promote pathogen virulence. Taken together, our data demonstrate that HopQ1 enhances bacterial virulence and associates with tomato 14-3-3 proteins in a phosphorylation-dependent manner that influences HopQ1’s subcellular localization and virulence-promoting activities in planta.

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Section: Discussionmentioning

confidence: 99%

The Pseudomonas syringae Effector HopQ1 Promotes Bacterial Virulence and Interacts with Tomato 14-3-3 Proteins in a Phosphorylation-Dependent Manner

Yadeta

Elmore

et al. 2013

Plant Physiology

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“…Furthermore, it has been shown that GF14 λ interacts with APX3 [70] and its over-expression in Arabidopsis increases drought stress tolerance [71]. Different 14-3-3 interacting proteins involved in response to stress or defense have been identified, which confer resistance to virus and fungal pathogens [72][73][74]. Recently, GF14 λ has been identified as a responsive phosphoprotein in a study on Arabidopsis proteome alterations upon P. syringae challenge [46].…”

Section: Methyl Viologen Treatmentmentioning

confidence: 99%

Response to biotic and oxidative stress in Arabidopsis thaliana: Analysis of variably phosphorylated proteins

Huang

Verrillo

Renzone

et al. 2011

Journal of Proteomics

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“…7 When BY2 cell lines are transformed with antisense constructs of Nt14-3-3h, the expression of the transgene is correlated with a strong inhibition of ROS accumulation following elicitation with cryptogein. This demonstrated the role of a 14-3-3 protein in the direct regulation of an oxidase during an incompatible interaction.…”

Section: Identification Of Regulators Of Plant Nadph Oxidasementioning

confidence: 99%

Regulation of Plant NADPH Oxidase

Elmayan

Simon-Plas

2007

Plant Signaling & Behavior

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The production of Reactive Oxygen Species (ROS) is one of the key events occurring during the response of plants to environmental changes, and contributing to establish adaptive signaling pathways. A plasma membrane bound NADPH oxidase enzyme has been evidenced as the ROS producing system in various plant-microorganisms interactions. We very recently reported, that a protein of the 14-3-3 family was able to interact directly with the C-terminus part of this NADPH oxidase, and that modification of its expression in tobacco cells led to reduced amount of ROS production upon elicitation. In this addendum, we summarize this work, present additional results, and propose an hypothetic model of regulation of this oxidase in a plant defense context.

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Members of 14-3-3 protein isoforms interacting with the resistance gene product N and the elicitor of Tobacco mosaic virus

Abstract: A functional cDNA for the resistance gene N

Cited by 63 publications

References 46 publications

The Pseudomonas syringae Effector HopQ1 Promotes Bacterial Virulence and Interacts with Tomato 14-3-3 Proteins in a Phosphorylation-Dependent Manner

The Pseudomonas syringae Effector HopQ1 Promotes Bacterial Virulence and Interacts with Tomato 14-3-3 Proteins in a Phosphorylation-Dependent Manner

Response to biotic and oxidative stress in Arabidopsis thaliana: Analysis of variably phosphorylated proteins

Regulation of Plant NADPH Oxidase

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