Metabolite profiling of Curcuma species grown in different regions using 1H NMR spectroscopy and multivariate analysis

Abstract

Curcuma is used to treat skin diseases and colic inflammatory disorders, and in insect repellants and antimicrobial and antidiabetic medications. Two Curcuma species (C. aromatica and C. longa) grown in Jeju-do and Jin-do were used in this study. Methanolic extracts were analyzed by (1)H NMR spectroscopy, and metabolite profiling coupled with multivariate analysis was applied to characterize the differences between species or origin. PCA analysis showed significantly greater differences between species than origins, and the metabolites responsible for the differences were identified. The concentrations of sugars (glucose, fructose, and sucrose) and essential oils (eucalyptol, curdione, and germacrone) were significantly different between the two species. However, the samples from Jeju-do and Jin-do were different mainly in their concentrations of organic acids (fumarate, succinate, acetate, and formate) and sugars. This study demonstrates that NMR-based metabolomics is an efficient method for fingerprinting and determining differences between Curcuma species or those grown in different regions.

Hepatic metabolic effects of Curcuma longa extract supplement in high-fructose and saturated fat fed rats

“…Curcuma can contain up to a few dozen micromolar concentrations of betaine 50 , which is insignificant compared to the levels of betaine found in this study, and can thus be neglected. The metabolism of betaine in the rat liver as a catabolic product of choline in and out of the mitochondria is represented in Fig.…”

Metabolic discrimination of sea buckthorn from different Hippophaë species by 1H NMR based metabolomics

“…Earlier researches also revealed that the concentrations of reducing sugars ( e . g ., glucose and fructose) and organic compounds in plant are related to temperature, low temperature will induce reducing sugars and organic compounds accumulation 39, 40 . However, this result was not completely repeated in the present study, such as malic acid level were higher in H .…”

Metabolic characterization of natural and cultured Ophicordyceps sinensis from different origins by 1H NMR spectroscopy

“…NMR has several advantages over other methods (LC/MS, GC/MS), including simple sample preparation, high reproducibility, non-selective, non-destructive and the capacity to elucidate structures [14]. Non-targeted metabolomic profiling based on 1 H NMR spectroscopy has been successfully applied to discriminate species or different origins of Curcuma [15], Radix Bupleuri [16], Danggui [17], and quality control of Polygonum [18], Pueraria [19], Angelica acutiloba roots [20] and Magnoliae officinalis cortex [21].…”