Method Development and Validation for estimation of Moxifloxacin HCl in tablet dosage form by RP-HPLC method

Abstract

is chemically 1-cyclopropyl-7-((s,s)-2,8diazabicyclo(4.3.0)non-8-yl)-6-fluoro-8-methoxy-1,4-dihydro-4-oxo-3 quinoline carboxylic acid. Moxifloxacin is an antibiotic used to treat respiratory infections, including acute sinusitis, acute exacerbations of chronic bronchitis, and community-acquired pneumonia, as well as dermatological infections, as a second-line agent in tuberculosis. The literature survey [1-7] reveals that there is some HPLC methods have been reported. In this paper we describe a simple, inexpensive, sensitive and validated HPLC [8-12] method for the determination of Moxifloxacin HCl in bulk and pharmaceutical formulation.Working standard of Moxifloxacin HCl was obtained from well reputed research laboratories. HPLC grade Methanol, Merck grade Orthophosphoric acid and Triethylamine and Milli-Q water were procured from the market. The separation was carried out on isocratic HPLC system with pre-packed Luna C-18, 5µ (250 x 4.6) mm column using filtered and degassed mixture of Buffer: Methanol (55:45) as mobile phase. Buffer PreparationTransferred 2 ml of orthophosphoric acid into 1000 ml of water and the P H were adjusted to 2.5 with triethylamine, filtered through 0.45µm nylon membrane filter and degassed. Mobile phaseBuffer and methanol were mixed in the ratio of 55:45 and sonicated to degas.

A validated stability-indicating high performance liquid chromatographic method for moxifloxacin hydrochloride and ketorolac tromethamine eye drops and its application in pH dependent degradation kinetics

“…[23] Linearity Aliquots of 0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4, 1.6, 1.8, and 2.0 mL of the stock solution of moxifloxacin hydrochloride and ketorolac tromethamine were transferred into a series of 10 mL volumetric flasks and diluted to the mark with water to yield 2, 4, 6,8,10,12,14,16,18, and 20 μg/mL each of moxifloxacin hydrochloride and ketorolac tromethamine. The calibration curve was constructed by plotting peak areas versus concentrations, and the regression equation was calculated.…”

“…Prostaglandins have been shown to be mediators of certain kinds of intraocular inflammation and also produce disruption of the blood-aqueous humour barrier, vasodilation, increased vascular permeability, leukocytosis, and increased intraocular pressure. [5,6] A detailed survey of analytical literature for moxifloxacin hydrochloride revealed several methods based on varied techniques, viz, HPLC, [7][8][9][10] Spectrophotometry, [11,12] High-Performance Thin-Layer Chromatography (HPTLC). [13,14] Similarly, a survey of the analytical literature for ketorolac tromethamine revealed several methods like HPLC, [15,16] HPTLC, [17,18] LC/MS/MS (Liquid Chromatography-Mass Spectrometry-Tandem Mass Spectrometry), [19] capillary electro chromatography.…”

Recent Trends in Analytical Techniques for the Development of Pharmaceutical Drugs

“…Statistical analysis proved the method was precise, reproducible, selective, specific, and accurate for analysis of GFC and AMB. The wide linearity range, sensitivity, accuracy, short retention time, and simple mobile phase imply the method is suitable for routine quality control of formulation products [27] recovery values for Atorvastatin, Ezetimibe and Fenofibrate ranged from 99.7-101.1%, 99.8-101.3% and 99.7-101.7%, respectively. The relative standard deviation for six replicates is always less than 2%.…”

Development and Validation of First Order Derivative Spectrophotometric Method for Simultaneous Estimation of Moxifloxacin and Prednisolone Acetate in Pharmaceutical Preparation

“…Literature review reveals RP-HPLC [7] , HPTLC [8] and spectrophotometry [9] methods for determination of MOX in alone. Prednisolone Acetate (PRD) (Figure 2) is chemically Pregna-1, 4-diene-3, 20-dione,21-(acetyloxy)-11,17-dihydroxy-,(11β).…”