Method transfer for fast liquid chromatography in pharmaceutical analysis: Application to short columns packed with small particle. Part I: Isocratic separation

Guillarme, Davy; Nguyen, Dao T.-T.; Rudaz, Serge; Veuthey, Jean‐Luc

doi:10.1016/j.ejpb.2006.11.027

Cited by 116 publications

(49 citation statements)

References 18 publications

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“…The column dimensions and particle diameters differed; the HPLC column was 100 mm × 4.6 mm, 3.5 m and the UHPLC column was 50 mm × 2.1 mm, 1.7 m. The flow rate and injection volume for HPLC was 2.0 mL/min and 10 L and for UHPLC they were scaled according to Ref. [31] which yielded 0.86 mL/min and 1 L. The scaling was done to keep the reduced linear velocity of the mobile phase constant. At these flow rates the pressure drop over the HPLC column was 165 bar and 570 bar over the UHPLC column.…”

Section: Practical Implicationsmentioning

confidence: 99%

Method transfer from high-pressure liquid chromatography to ultra-high-pressure liquid chromatography. II. Temperature and pressure effects

Åsberg

Samuelsson

Leśko

et al. 2015

Journal of Chromatography A

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Section: Practical Implicationsmentioning

confidence: 99%

Method transfer from high-pressure liquid chromatography to ultra-high-pressure liquid chromatography. II. Temperature and pressure effects

Åsberg

Samuelsson

Leśko

et al. 2015

Journal of Chromatography A

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“…There is, however, only one previous report presenting the use of UHPLC for separation and quantification of OA and UA [28]. Despite the separation of both analytes has been achieved in a relatively short time of 7.2 min, the basic rules of HPLC-to-UPLC method transfer suggest that further improvement might be expected [29]. Optimisation of the (U)HPLC separation is a complex process influenced by numerous factors, such as mobile phase composition, flow rate, column temperature, detector wavelength, etc.…”

Section: Introductionmentioning

confidence: 99%

Application of Response Surface Methodology for Optimisation of Simultaneous UHPLC-PDA Determination of Oleanolic and Ursolic Acids and Standardisation of Ericaceae Medicinal Plants

et al. 2016

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Abstract:A fast and sensitive ultra-high performance liquid chromatography-photodiode array (UHPLC-PDA) method for simultaneous quantification of oleanolic acid (OA) and ursolic acid (UA) in plant materials was developed. A central composite design combined with a response surface methodology was utilized to establish optimal separation conditions. The final separation was accomplished on a Zorbax Eclipse XDB-C18 column (1.8 µm, 100 mm × 3 mm I.D., Agilent, Santa Clara, CA, USA) using a mixture 90:10 (v/v) of methanol and 1% (w/v) aqueous orthophosporic acid as a mobile phase at a flow rate of 0.44 mL/min and temperature of 18 • C. The analysis was completed in 6.2 min with satisfactory resolution of 1.5 between the target analytes. The developed method proved to be precise (relative standard deviations below 3.2%), accurate (recoveries in the range of 95.27%-98.60%), and sensitive (limits of detection (LODs) in the range of 0.047-0.051 mg/mL). The method was then successfully applied to evaluate OA and UA content in real samples of selected Ericaceae plant materials (leaves of Arctostaphylos uva ursi, Vaccinium myrtillus, Vaccinium vitis idaea, Gaultheria procumbens). The content of OA and UA in investigated samples varied in the range of 0.74-4.47 mg/g dry weight (dw) and 1.30-18.61 mg/g dw, respectively.

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“…[34][35][36][37] It was found that minimization of extra-column broadening and adjustments in injection volume and flow rate are necessary for the conversion of conventional HPLC isocratic methods to UHPLC isocratic methods. [34] Adjustments in injection volume, flow rate, and gradient with minimization of the dwell volume are required for the conversion of conventional HPLC gradient methods to UHPLC gradient methods. [35] Pharmaceutical Applications UHPLC with highly efficient sub-2 mm particulate columns is evolving to become a high-speed replacement for conventional HPLC in the analysis of pharmaceuticals.…”

Section: Ultra-high Performance Liquid Chromatography With Sub-2 Lm Cmentioning

confidence: 99%

Developments in Fast Liquid Chromatographic Analysis of Pharmaceuticals

Natishan

2011

Journal of Liquid Chromatography & Related Technologies

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& The need for pharmaceutical analytical laboratories to increase sample throughput and speed of analysis to meet accelerated drug development timelines has led to considerable interest in the development of fast liquid chromatographic technology. Fast liquid chromatographic pharmaceutical analysis is accomplished by developments in ultra-fast high performance liquid chromatographic instrumentation, emerging technologies in column stationary phases and high temperature liquid chromatographic analysis. This review provides an overview and gives recent developments and applications in the different strategies used in fast liquid chromatographic pharmaceutical analysis.

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Method transfer for fast liquid chromatography in pharmaceutical analysis: Application to short columns packed with small particle. Part I: Isocratic separation

Cited by 116 publications

References 18 publications

Method transfer from high-pressure liquid chromatography to ultra-high-pressure liquid chromatography. II. Temperature and pressure effects

Method transfer from high-pressure liquid chromatography to ultra-high-pressure liquid chromatography. II. Temperature and pressure effects

Application of Response Surface Methodology for Optimisation of Simultaneous UHPLC-PDA Determination of Oleanolic and Ursolic Acids and Standardisation of Ericaceae Medicinal Plants

Developments in Fast Liquid Chromatographic Analysis of Pharmaceuticals

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