Methyl viologen responsive proteome dynamics ofAnabaenasp. strain PCC7120

Abstract

A proteomic approach was employed to elucidate the response of an agriculturally important microbe, Anabaena sp. strain PCC7120, to methyl viologen (MV). Exposure to 2 μM MV caused 50% lethality (LD50 ) within 6 h and modified the cellular levels of several proteins. About 31 proteins increased in abundance and 24 proteins decreased in abundance, while 55 proteins showed only a minor change in abundance. Of these, 103 proteins were identified by MS. Levels of proteins involved in ROS detoxification and chaperoning activities were enhanced but that of crucial proteins involved in light and dark reactions of photosynthesis declined or constitutive. The abundance of proteins involved in carbon and energy biogenesis were altered. The study elaborated the oxidative stress defense mechanism deployed by Anabaena, identified carbon metabolism and energy biogenesis as possible major targets of MV sensitivity, and suggested potential biotechnological interventions for improved stress tolerance in Anabaena 7120.

Involvement of phosphoesterases in tributyl phosphate degradation in Sphingobium sp. strain RSMS

“…The oligopeptide samples were analysed by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (UltraFlex III MALDI ToF/ToF mass spectrometer, Bruker Daltonics, Germany) in MS mode, as per the manufacturer's instructions and exactly as per the calibration and spectral acquisition protocol described earlier (Panda et al 2014). Similarity searches were carried out using MASCOT search engine (Matrix Science, UK) using the NCBI non-redundant database.…”

“…The proteins were applied to 3-10 NL IPG strips, 17 cm (BioRad, India), by cup-loading method, as per the manufacturer's instructions and as detailed earlier (Anaganti et al 2014;Panda et al 2014). Second dimensional resolution was achieved by 14 % SDS-PAGE.…”

Exploring the membrane proteome of the diazotropic cyanobacterium Anabaena PCC7120 through gel-based proteomics and in silico approaches

“…Due to this complex subcellular membrane organization in cyanobacteria very little is known about the process of extra-cytoplasmic protein sorting. Although cytosolic proteome of this organism has been explored to a large extent [19][20][21][22][23][24][25] there are only few reports [26,27] dealing with membrane proteins and those have employed primarily gel less approach. Employing gel less approach a total of 55 and 18 proteins were identified in the outer membrane fraction of vegetative cells and hetrocysts of Anabaena [26,27].…”

“…Of these, thirteen proteins namely Alr3608 (spot no 1), Alr0525 (spot no 4, 23, 24), All1743 (spot no 25), All2453 (spot no 42), Alr2877 (spot no18,19,26,27,50,55), Alr2887 (spot no15), All0070 (spot no 46, 49), All3854 (spot no 53), Alr1524 (spot no 31), All4337 (spot no 17), All0258 (spot no37), All0259(spot no 44) and Alr4550 (spot no 45, 47, 48) contain 1-TM segment while the remaining three proteins All4499 (spot no 16), All0109 (spot no 32) and Alr2264 (spot no2) contain 2, 3 and 9-TM helices respectively.A group of 20 different TM helix containing proteins were identified in the third experiment. Of these 12 proteins namely All1355 (spot no 1), All3854 (spot no 32, 33), Alr2877 (spot no19), Alr2887 (spot no 37, 38), Alr4550 (spot no5,7,10,11,12,13,20,21,22,23,25,26,30), All7614 (spot no 36), All4337 (spot no 47), Alr2055 (spot no 29), Alr2903 (spot no 43), Alr2751 (spot no 42), Alr1819 (spot no 34) and Alr1524 (spot no 35) contained 1-TM helix; 3 proteins, All4499 (spot no14,15,16,18), All2452 (spot no33) and Alr2269 (spot no45) contained 2-TM helix each; the other two i.e. All4575 (spot no 3) and…”

A Salt-Inducible Mn-Catalase (KatB) Protects Cyanobacterium from Oxidative Stress

“…Thus, studying the detailed basis of oxidative stress resistance in Anabaena is essential for development of novel biofertilizers, which may be used under adverse conditions. The role played by various proteins in overcoming oxidative stress in the filamentous, heterocystous, nitrogen-fixing, cyanobacterium Anabaena PCC 7120 has garnered remarkable interest in the recent past (Cha et al, 2007;Agrawal et al, 2014;Panda et al, 2014;Banerjee et al, 2015;Tailor and Ballal, 2015).…”